EC: 2.3.3.10

hydroxymethylglutaryl-CoA synthase (acetyl-CoA:acetoacetyl-CoA C-acetyltransferase (thioester-hydrolysing, carboxymethyl-forming))

enzyme lambda metabolic reaction experiment
- [hydroxymethylglutaryl-CoA synthase(Enzyme) wildtype]

S_(3_Oxobutyl)_CoA + Acetyl_CoA + H2O -> Coenzyme_A + S_(4_Carboxy_3_hydroxy_isoamyl)_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-Cl, 0.1 mM EDTA
PH: 8.2
Temperature: 30
uniprot:P54869 [hydroxymethylglutaryl-CoA synthase(Enzyme) wildtype FLAG-tag]

H2O + Acetoacetyl_CoA + Acetyl_CoA -> Coenzyme_A + 3_Hydroxy_3_methylglutaryl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 70 mM Tris-Cl, 130 µM DTNB
PH: 8
Temperature: 36

Pathways

pathway id name
BioCyc:HUMAN_PWY66-367 ketogenesis
BioCyc:META_PWY-922 mevalonate pathway I
BioCyc:MOUSE_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:MOUSE_PWY66-367 ketogenesis
BioCyc:MSMI420247_PWY-6174 mevalonate pathway II (archaea)
BioCyc:HUMAN_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:HUMAN_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
BioCyc:YEAST_ERGOSTEROL-SYN-PWY superpathway of ergosterol biosynthesis
BioCyc:YEAST_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
BioCyc:YEAST_PWY-922 mevalonate pathway
BioCyc:ARA_PWY-922 mevalonate pathway I
BioCyc:MSMI420247_PWY-922 mevalonate pathway I
BioCyc:META_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
BioCyc:META_PWY-7571 ferrichrome A biosynthesis
BioCyc:META_PWY-7391 isoprene biosynthesis II (engineered)
BioCyc:HUMAN_PWY-922 mevalonate pathway
BioCyc:META_PWY66-367 ketogenesis
BioCyc:META_ERGOSTEROL-SYN-PWY superpathway of ergosterol biosynthesis I
BioCyc:META_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:MOUSE_PWY3FA-941 ketogenesis
BioCyc:MOUSE_PWY-922 mevalonate pathway I
BioCyc:MSMI420247_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
PlantCyc:ARA_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
PlantCyc:PLANT_PWY-922 mevalonate pathway I
PlantCyc:PLANT_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
PlantCyc:ARA_PWY-922 mevalonate pathway I
Reactome:R-HSA-9969896 Lanosterol biosynthesis
Reactome:R-MMU-9969896 Lanosterol biosynthesis
Reactome:R-SPO-9969896 Lanosterol biosynthesis
Reactome:R-DDI-9969896 Lanosterol biosynthesis
Reactome:R-CEL-9969896 Lanosterol biosynthesis
Reactome:R-RNO-9969896 Lanosterol biosynthesis
Reactome:R-SCE-9969896 Lanosterol biosynthesis
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-CEL-191273 Cholesterol biosynthesis
Reactome:R-CEL-77111 Synthesis of Ketone Bodies
Reactome:R-DDI-191273 Cholesterol biosynthesis
Reactome:R-HSA-400206 Regulation of lipid metabolism by PPARalpha
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-MMU-77111 Synthesis of Ketone Bodies
Reactome:R-RNO-74182 Ketone body metabolism
Reactome:R-SCE-8957322 Metabolism of steroids
Reactome:R-SCE-74182 Ketone body metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-74182 Ketone body metabolism
Reactome:R-SSC-74182 Ketone body metabolism
Reactome:R-BTA-77111 Synthesis of Ketone Bodies
Reactome:R-CEL-556833 Metabolism of lipids
Reactome:R-CEL-8957322 Metabolism of steroids
Reactome:R-CEL-74182 Ketone body metabolism
Reactome:R-DDI-8957322 Metabolism of steroids
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-191273 Cholesterol biosynthesis
Reactome:R-HSA-2426168 Activation of gene expression by SREBF (SREBP)
Reactome:R-HSA-77111 Synthesis of Ketone Bodies
Reactome:R-MMU-74182 Ketone body metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SSC-1430728 Metabolism
Reactome:R-SSC-556833 Metabolism of lipids
Reactome:R-HSA-1989781 PPARA activates gene expression
Reactome:R-MMU-191273 Cholesterol biosynthesis
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-8957322 Metabolism of steroids
Reactome:R-RNO-77111 Synthesis of Ketone Bodies
Reactome:R-SCE-556833 Metabolism of lipids
Reactome:R-SCE-191273 Cholesterol biosynthesis
Reactome:R-SCE-77111 Synthesis of Ketone Bodies
Reactome:R-SPO-8957322 Metabolism of steroids
Reactome:R-SPO-77111 Synthesis of Ketone Bodies
Reactome:R-SSC-77111 Synthesis of Ketone Bodies
Reactome:R-BTA-74182 Ketone body metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-556833 Metabolism of lipids
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-1655829 Regulation of cholesterol biosynthesis by SREBP (SREBF)
Reactome:R-HSA-74182 Ketone body metabolism
Reactome:R-RNO-191273 Cholesterol biosynthesis
Reactome:R-SPO-556833 Metabolism of lipids
Reactome:R-SPO-191273 Cholesterol biosynthesis
WikiPathways:WP5175 Disorders in ketone body synthesis
WikiPathways:WP5195 Disorders in ketolysis
WikiPathways:WP5329 Cholesterol biosynthesis pathway in hepatocytes
WikiPathways:WP5220 Metabolic reprogramming in pancreatic cancer
WikiPathways:WP4190 Mevalonate arm of cholesterol biosynthesis pathway
PathBank:SMP0120662 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0120806 3-Hydroxyisobutyric Aciduria
PathBank:SMP0120440 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0120444 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0120464 CHILD Syndrome
PathBank:SMP0120472 Desmosterolosis
PathBank:SMP0120512 Lysosomal Acid Lipase Deficiency (Wolman Disease)
PathBank:SMP0120516 Maple Syrup Urine Disease
PathBank:SMP0120573 Cholesteryl Ester Storage Disease
PathBank:SMP0120589 Isovaleric Acidemia
PathBank:SMP0120633 Succinyl CoA: 3-Ketoacid CoA Transferase Deficiency
PathBank:SMP0000032 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0000173 beta-Ketothiolase Deficiency
PathBank:SMP0000199 Maple Syrup Urine Disease
PathBank:SMP0000384 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000141 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0000524 Isovaleric Acidemia
PathBank:SMP0002438 Cholesterol Biosynthesis and Metabolism CE(12:0)
PathBank:SMP0087185 Butyrate Metabolism
PathBank:SMP0087234 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0120661 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0120665 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0120745 Methylmalonic Aciduria
PathBank:SMP0120757 Propionic Acidemia
PathBank:SMP0120805 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0120439 2-Methyl-3-hydroxybutryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120443 3-Methylglutaconic Aciduria Type III
PathBank:SMP0120459 beta-Ketothiolase Deficiency
PathBank:SMP0120503 Isovaleric Aciduria
PathBank:SMP0120515 Smith-Lemli-Opitz Syndrome (SLOS)
PathBank:SMP0120523 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120576 Wolman Disease
PathBank:SMP0120588 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0121055 Mevalonate Pathway
PathBank:SMP0121174 Ketone Body Metabolism
PathBank:SMP0000138 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0000140 3-Methylglutaconic Aciduria Type III
PathBank:SMP0000238 Isovaleric Aciduria
PathBank:SMP0000523 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0002436 Cholesterol biosynthesis and metabolism CE(10:0)
PathBank:SMP0002441 Cholesterol biosynthesis and metabolism CE(18:0)
PathBank:SMP0063603 Butyrate Metabolism
PathBank:SMP0063689 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0120660 2-Methyl-3-hydroxybutryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120664 3-Methylglutaconic Aciduria Type III
PathBank:SMP0120736 Maple Syrup Urine Disease
PathBank:SMP0120808 Isovaleric Acidemia
PathBank:SMP0120852 Succinyl CoA: 3-Ketoacid CoA Transferase Deficiency
PathBank:SMP0070041 Cholesterol Biosynthesis and Metabolism
PathBank:SMP0120441 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0120465 Chondrodysplasia Punctata II, X-Linked Dominant (CDPX2)
PathBank:SMP0120525 Methylmalonic Aciduria
PathBank:SMP0120537 Propionic Acidemia
PathBank:SMP0120549 Hypercholesterolemia
PathBank:SMP0120574 Hyper-IgD Syndrome
PathBank:SMP0120586 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0000137 2-Methyl-3-hydroxybutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000237 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0000200 Methylmalonic Aciduria
PathBank:SMP0000521 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0002383 Ketone Body Metabolism
PathBank:SMP0120663 3-Methylglutaconic Aciduria Type I
PathBank:SMP0120679 beta-Ketothiolase Deficiency
PathBank:SMP0120723 Isovaleric Aciduria
PathBank:SMP0120743 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120807 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120442 3-Methylglutaconic Aciduria Type I
PathBank:SMP0120575 Mevalonic Aciduria
PathBank:SMP0120587 3-Hydroxyisobutyric Aciduria
PathBank:SMP0121209 Mevalonate Pathway
PathBank:SMP0000236 Propionic Acidemia
PathBank:SMP0000139 3-Methylglutaconic Aciduria Type I
PathBank:SMP0000522 3-Hydroxyisobutyric Aciduria
PathBank:SMP0002384 Terpenoid Backbone Biosynthesis
PathBank:SMP0002435 Cholesterol biosynthesis and metabolism CE(14:0)
PathBank:SMP0002440 Cholesterol Biosynthesis and Metabolism CE(16:0)
PathBank:SMP0087202 Ketone Body Metabolism
PathBank:SMP0087287 Butyrate Metabolism
PathBank:SMP0087299 Ketone Body Metabolism
PathBank:SMP0087327 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0012467 Butanoate Metabolism
PathBank:SMP0063631 Ketone Body Metabolism
PathBank:SMP0063670 Steroid Biosynthesis