alkB
K03919 alkB; DNA oxidative demethylase [EC:1.14.11.33]
Protein Function: DNA oxidative demethylase [EC:1.14.11.33]
DBLinks
- KEGG: K03919
- EC Number: 1.14.11.33
Proteins
| protein id | name | function | taxonomy source |
|---|---|---|---|
| A0A0E0GVK0 | A0A0E0GVK0_ORYNI | Fe2OG dioxygenase domain-containing protein | Oryza nivara |
| A0A1Y3PAT0 | A0A1Y3PAT0_9PSED | Alpha-ketoglutarate-dependent dioxygenase AlkB | Pseudomonas caspiana |
| A0A4U0SSI8 | A0A4U0SSI8_9ACTN | Alpha-ketoglutarate-dependent dioxygenase AlkB | Actinacidiphila oryziradicis |
| A0A6B9FFR5 | A0A6B9FFR5_9HYPH | Alpha-ketoglutarate-dependent dioxygenase AlkB | Methylobacterium mesophilicum SR1.6/6 |
| A0A8J8XY26 | A0A8J8XY26_ORYSJ | Fe2OG dioxygenase domain-containing protein | Oryza sativa Japonica Group |
| A0A9R1H9E9 | A0A9R1H9E9_WHEAT | Fe2OG dioxygenase domain-containing protein | Triticum aestivum |
| A0A9R1HNL0 | A0A9R1HNL0_WHEAT | DNA N(6)-methyladenine demethylase | Triticum aestivum |
| A0A9R1L353 | A0A9R1L353_WHEAT | Fe2OG dioxygenase domain-containing protein | Triticum aestivum |
| A0AAE8HRL1 | A0AAE8HRL1_9HYPH | Alkylated DNA repair protein (DNA oxidative demethylase) | Methylobacterium phyllosphaerae |
| A0ABR4S8Z8 | A0ABR4S8Z8_9ACTN | DNA repair protein | Streptomyces wadayamensis |
Reactions
| reaction id | name | note |
|---|---|---|
| BioCyc:RXN0-7484 | CPD0-2726 + 2-KETOGLUTARATE + OXYGEN-MOLECULE --> DNA-Adenines + CPD-21542 + CARBON-DIOXIDE + SUC | |
| BioCyc:RXN0-986 | N1-ETHYLADENINE-IN-DNA + 2-KETOGLUTARATE + OXYGEN-MOLECULE --> DNA-Adenines + ACETALD + SUC + CARBON-DIOXIDE | AlkB catalyzes repair of DNA lesions caused by SN2 methylating compounds, rather than lesions caused by SN1 methylating compounds or ionizing radiation |CITS: [10950872]|. AlkB preferentially binds to and repairs methylation lesions in single-stranded DNA rather than double-stranded DNA, suggesting that AlkB repair activity may be specific for the single-stranded DNA present during transcription and DNA replication |CITS: [10950872]|. AlkB also catalyzes repair of methylated RNA |CITS: [12594517]|. |
| BioCyc:RXN-18739 | CPD-20035 + 2-KETOGLUTARATE + OXYGEN-MOLECULE --> DAMP + FORMALDEHYDE + SUC + CARBON-DIOXIDE | |
| BioCyc:RXN0-7280 | CPD-20036 + 2-KETOGLUTARATE + OXYGEN-MOLECULE --> DATP + FORMALDEHYDE + SUC + CARBON-DIOXIDE | |
| BioCyc:RXN-18737 | an-Nsup1sup-methyladenine-in-DNA + 2-KETOGLUTARATE + OXYGEN-MOLECULE --> DNA-Adenines + CARBON-DIOXIDE + FORMALDEHYDE + SUC | |
| BioCyc:RXN0-984 | N1-METHYLADENINE + OXYGEN-MOLECULE + 2-KETOGLUTARATE --> ADENINE + CARBON-DIOXIDE + FORMALDEHYDE + SUC | AlkB preferentially binds to and repairs methylation lesions in single-stranded DNA rather than double-stranded DNA, suggesting that AlkB repair activity may be specific for the single-stranded DNA present during transcription and DNA replication |CITS: [10950872]|. AlkB also catalyzes repair of methylated RNA |CITS: [12594517]|. AlkB-mediated repair involves hydroxylation of methylation lession coupled to decarboxylation of α-ketoglutarate, an unusual repair mechanism that regenerates the intact, unmethylated base |CITS: [12226667], [12226668]|. |
| BioCyc:RXN-18738 | an-Nsup3sup-methylcytosine-in-DNA + 2-KETOGLUTARATE + OXYGEN-MOLECULE --> DNA-Cytosines + CARBON-DIOXIDE + FORMALDEHYDE + SUC + PROTON | |
| BioCyc:RXN-12353 | Methylated-DNA-Bases + OXYGEN-MOLECULE + 2-KETOGLUTARATE --> Nucleobases-in-DNA + CARBON-DIOXIDE + FORMALDEHYDE + SUC | |
| Rhea:RHEA:30300 | a methylated nucleobase within DNA + 2-oxoglutarate + O2 => a nucleobase within DNA + formaldehyde + succinate + CO2 | |
| Rhea:RHEA:30301 | a nucleobase within DNA + formaldehyde + succinate + CO2 => a methylated nucleobase within DNA + 2-oxoglutarate + O2 |