EC: 4.2.1.93

ATP-dependent NAD(P)H-hydrate dehydratase ((6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine-dinucleotide hydro-lyase (ATP-hydrolysing; NADH-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q9CZ42 [ATP-dependent NAD(P)H-hydrate dehydratase(Enzyme) mutant Carkd (lacking mitochondrail propeptide), N-terminal His-tag]

(6S)_6_beta_Hydroxy_1,4,5,6_tetrahydronicotinamide_adenine_dinucleotide + ATP -> Phosphate + NADH + ADP + H+		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 2 mM MgCl2, 0.05-0.2 mg/ml BSA
PH: 8
Temperature: 30
uniprot:Q9CZ42 [ATP-dependent NAD(P)H-hydrate dehydratase(Enzyme) mutant Carkd (lacking mitochondrail propeptide), N-terminal His-tag]

ATP + (6S)_6_beta_Hydroxy_1,4,5,6_tetrahydronicotinamide_adenine_dinucleotide_2'_phosphate -> Phosphate + ADP + H+ + NADPH		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 2 mM MgCl2, 0.05-0.2 mg/ml BSA
PH: 8
Temperature: 30
uniprot:Q9CZ42 [ATP-dependent NAD(P)H-hydrate dehydratase(Enzyme) mutant Carkd (lacking mitochondrail propeptide), N-terminal His-tag]

(6S)_6_beta_Hydroxy_1,4,5,6_tetrahydronicotinamide_adenine_dinucleotide + ATP -> ADP + Phosphate + H+ + NADH		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 2 mM MgCl2, 0.05-0.2 mg/ml BSA
PH: 8
Temperature: 30
uniprot:Q8IW45 [Mg2+]

ATP + (6S)_6_beta_Hydroxy_1,4,5,6_tetrahydronicotinamide_adenine_dinucleotide -> Phosphate + NADH + ADP + H+		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM HEPES
PH: 7.1
Temperature: 37
uniprot:Q8IW45 [Mg2+]

(6S)_6_beta_Hydroxy_1,4,5,6_tetrahydronicotinamide_adenine_dinucleotide + ATP -> Phosphate + NADH + ADP + H+		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM HEPES
PH: 7.1
Temperature: 37
uniprot:Q8IW45 [Mg2+]

(6S)_6_beta_Hydroxy_1,4,5,6_tetrahydronicotinamide_adenine_dinucleotide + ATP -> H+ + ADP + Phosphate + NADH		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM HEPES
PH: 7.1
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-6938 NADH repair
BioCyc:META_PWY-6938 NADH repair
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-196807 Nicotinate metabolism
Reactome:R-CEL-196807 Nicotinate metabolism
Reactome:R-DRE-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-DDI-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-DME-196807 Nicotinate metabolism
Reactome:R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-RNO-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SCE-196807 Nicotinate metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-BTA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-CEL-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-DRE-196854 Metabolism of vitamins and cofactors
Reactome:R-DDI-196854 Metabolism of vitamins and cofactors
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-RNO-196854 Metabolism of vitamins and cofactors
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SPO-196807 Nicotinate metabolism
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-196807 Nicotinate metabolism
Reactome:R-DDI-196807 Nicotinate metabolism
Reactome:R-HSA-196807 Nicotinate metabolism
Reactome:R-MMU-196807 Nicotinate metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-196807 Nicotinate metabolism
Reactome:R-SPO-196854 Metabolism of vitamins and cofactors
Reactome:R-BTA-196854 Metabolism of vitamins and cofactors
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-196854 Metabolism of vitamins and cofactors
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DME-196854 Metabolism of vitamins and cofactors
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SCE-196854 Metabolism of vitamins and cofactors
Reactome:R-SPO-196849 Metabolism of water-soluble vitamins and cofactors