uniprot:P07902 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) mutant E220K]
UDP_D_glucose + alpha_D_Galactose_1_phosphate -> alpha_D_Glucose_1_phosphate + UDP_D_galactose |
(( (Vmax * S) ) / (S + Km)) |
|
buffer: 100 mM Glycine, 5 mM MgCl2 , 5 mM dithiothreitol, 0.8 mM NADP, 5 μM glucose-alpha-1,6-diphosphate, 0.5 U/ml Phosphoglucomutase, 0.5 U/ml Glucose-6-phosphate dehydrogenase
PH: 8.7
Temperature: 36 |
uniprot:P07902 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) mutant R201C]
alpha_D_Galactose_1_phosphate + UDP_D_glucose -> UDP_D_galactose + alpha_D_Glucose_1_phosphate |
(( (Vmax * S) ) / (S + Km)) |
|
buffer: 100 mM Glycine, 5 mM MgCl2 , 5 mM dithiothreitol, 0.8 mM NADP, 5 μM glucose-alpha-1,6-diphosphate, 0.5 U/ml Phosphoglucomutase, 0.5 U/ml Glucose-6-phosphate dehydrogenase
PH: 8.7
Temperature: 36 |
uniprot:P07902 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) wildtype]
alpha_D_Galactose_1_phosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UDP_D_galactose |
(( (Vmax * S) ) / (S + Km)) |
|
buffer: 100 mM Glycine, 5 mM MgCl2 , 5 mM dithiothreitol, 0.8 mM NADP, 5 μM glucose-alpha-1,6-diphosphate, 0.5 U/ml Phosphoglucomutase, 0.5 U/ml Glucose-6-phosphate dehydrogenase
PH: 8.7
Temperature: 36 |
uniprot:P07902 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) wildtype]
alpha_D_Galactose_1_phosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UDP_D_galactose |
(( (Vmax * S) ) / (S + Km)) |
|
buffer: 100 mM Glycine, 5 mM MgCl2 , 5 mM dithiothreitol, 0.8 mM NADP, 5 μM glucose-alpha-1,6-diphosphate, 0.5 U/ml Phosphoglucomutase, 0.5 U/ml Glucose-6-phosphate dehydrogenase
PH: 8.7
Temperature: 36 |
uniprot:P07902 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) mutant R201C]
UDP_D_glucose + alpha_D_Galactose_1_phosphate -> alpha_D_Glucose_1_phosphate + UDP_D_galactose |
(( (Vmax * S) ) / (S + Km)) |
|
buffer: 100 mM Glycine, 5 mM MgCl2 , 5 mM dithiothreitol, 0.8 mM NADP, 5 μM glucose-alpha-1,6-diphosphate, 0.5 U/ml Phosphoglucomutase, 0.5 U/ml Glucose-6-phosphate dehydrogenase
PH: 8.7
Temperature: 36 |
uniprot:P07902 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) mutant E220K]
alpha_D_Galactose_1_phosphate + UDP_D_glucose -> UDP_D_galactose + alpha_D_Glucose_1_phosphate |
(( (Vmax * S) ) / (S + Km)) |
|
buffer: 100 mM Glycine, 5 mM MgCl2 , 5 mM dithiothreitol, 0.8 mM NADP, 5 μM glucose-alpha-1,6-diphosphate, 0.5 U/ml Phosphoglucomutase, 0.5 U/ml Glucose-6-phosphate dehydrogenase
PH: 8.7
Temperature: 36 |
uniprot:P07902 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) mutant E291V]
UDP_D_glucose + alpha_D_Galactose_1_phosphate -> alpha_D_Glucose_1_phosphate + UDP_D_galactose |
(( (Vmax * S) ) / (S + Km)) |
|
buffer: 100 mM Glycine, 5 mM MgCl2 , 5 mM dithiothreitol, 0.8 mM NADP, 5 μM glucose-alpha-1,6-diphosphate, 0.5 U/ml Phosphoglucomutase, 0.5 U/ml Glucose-6-phosphate dehydrogenase
PH: 8.7
Temperature: 36 |
uniprot:P07902 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) mutant R223S]
UDP_D_glucose + alpha_D_Galactose_1_phosphate -> UDP_D_galactose + alpha_D_Glucose_1_phosphate |
(( (Vmax * S) ) / (S + Km)) |
|
buffer: 100 mM Glycine, 5 mM MgCl2 , 5 mM dithiothreitol, 0.8 mM NADP, 5 μM glucose-alpha-1,6-diphosphate, 0.5 U/ml Phosphoglucomutase, 0.5 U/ml Glucose-6-phosphate dehydrogenase
PH: 8.7
Temperature: 36 |
uniprot:P07902 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) mutant E291V]
alpha_D_Galactose_1_phosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UDP_D_galactose |
(( (Vmax * S) ) / (S + Km)) |
|
buffer: 100 mM Glycine, 5 mM MgCl2 , 5 mM dithiothreitol, 0.8 mM NADP, 5 μM glucose-alpha-1,6-diphosphate, 0.5 U/ml Phosphoglucomutase, 0.5 U/ml Glucose-6-phosphate dehydrogenase
PH: 8.7
Temperature: 36 |
uniprot:P07902 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) mutant I278N]
alpha_D_Galactose_1_phosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UDP_D_galactose |
(( (Vmax * S) ) / (S + Km)) |
|
buffer: 100 mM Glycine, 5 mM MgCl2 , 5 mM dithiothreitol, 0.8 mM NADP, 5 μM glucose-alpha-1,6-diphosphate, 0.5 U/ml Phosphoglucomutase, 0.5 U/ml Glucose-6-phosphate dehydrogenase
PH: 8.7
Temperature: 36 |
uniprot:P07902 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) mutant L289F]
alpha_D_Galactose_1_phosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UDP_D_galactose |
(( (Vmax * S) ) / (S + Km)) |
|
buffer: 100 mM Glycine, 5 mM MgCl2 , 5 mM dithiothreitol, 0.8 mM NADP, 5 μM glucose-alpha-1,6-diphosphate, 0.5 U/ml Phosphoglucomutase, 0.5 U/ml Glucose-6-phosphate dehydrogenase
PH: 8.7
Temperature: 36 |
uniprot:P07902 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) mutant I278N]
alpha_D_Galactose_1_phosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UDP_D_galactose |
(( (Vmax * S) ) / (S + Km)) |
|
buffer: 100 mM Glycine, 5 mM MgCl2 , 5 mM dithiothreitol, 0.8 mM NADP, 5 μM glucose-alpha-1,6-diphosphate, 0.5 U/ml Phosphoglucomutase, 0.5 U/ml Glucose-6-phosphate dehydrogenase
PH: 8.7
Temperature: 36 |
uniprot:P07902 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) mutant L289F]
UDP_D_glucose + alpha_D_Galactose_1_phosphate -> UDP_D_galactose + alpha_D_Glucose_1_phosphate |
(( (Vmax * S) ) / (S + Km)) |
|
buffer: 100 mM Glycine, 5 mM MgCl2 , 5 mM dithiothreitol, 0.8 mM NADP, 5 μM glucose-alpha-1,6-diphosphate, 0.5 U/ml Phosphoglucomutase, 0.5 U/ml Glucose-6-phosphate dehydrogenase
PH: 8.7
Temperature: 36 |
uniprot:P07902 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) mutant R223S]
alpha_D_Galactose_1_phosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UDP_D_galactose |
(( (Vmax * S) ) / (S + Km)) |
|
buffer: 100 mM Glycine, 5 mM MgCl2 , 5 mM dithiothreitol, 0.8 mM NADP, 5 μM glucose-alpha-1,6-diphosphate, 0.5 U/ml Phosphoglucomutase, 0.5 U/ml Glucose-6-phosphate dehydrogenase
PH: 8.7
Temperature: 36 |
uniprot:P09148 [Fe2+]
alpha_D_Galactose_1_phosphate + UDPglucose -> D_Glucose_1_phosphate + UDPgalactose |
(( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (Ka * B) ) + (Kb * A)) ) + (A * B))) |
|
buffer: 95 mM sodium bicinate, 8 mM cysteine, 0.326 mM TPN+, 3.3 µM glucose-1,6-diphosphate, phosphoglucomutase, glucose-6-phosphate dehydrogenase, 10 mM beta-mercaptoethanol
PH: 8.5
Temperature: 27 |
uniprot:P09148 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) wildtype]
alpha_D_Glucose_1_phosphate + UDP_D_galactose -> alpha_D_Galactose_1_phosphate + UDP_D_glucose |
(1 / (( (( (1 / V) ) * (( (1 + (Km_A / A)) ) + (( (( (Km_A * Q) ) / Ki_Q) ) * A))) ) + (( (Km_B / V) ) + (( (( (( (( (Km_A * Ki_B) ) * Q) ) / V) ) * Ki_Q) ) * A)))) |
|
buffer: 95 mM sodium bicinate, 8 mM cysteine
PH: 8.5
Temperature: 27 |
uniprot:P09148 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) wildtype]
alpha_D_Glucose_1_phosphate + UDP_D_galactose -> UDP_D_glucose + alpha_D_Galactose_1_phosphate |
(1 / (( (( (1 / V) ) * (1 + (Km_A / A))) ) + (( (( (( (Km_B / V) ) * (( (1 + (P / Ki_P)) ) + (( (( (Ki_A * P) ) / Ki_P) ) * A))) ) * 1) ) / B))) |
|
buffer: 95 mM sodium bicinate, 8 mM cysteine, 0.382 mM NAD+, excess UDP-glucose dehydrogenase
PH: 8.5
Temperature: 27 |
uniprot:P09148 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) wildtype]
UDP_D_glucose + alpha_D_Galactose_1_phosphate -> UDP_D_galactose + alpha_D_Glucose_1_phosphate |
(1 / (( (( (1 / V) ) + (( (Km_B / V) ) * B)) ) + (( (( (Km_A / V) ) * A) ) * (1 + (B / KI_B))))) |
|
buffer: 95 mM sodium bicinate, 8 mM cysteine
PH: 8.5
Temperature: 27 |
uniprot:P09148 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) wildtype]
alpha_D_Glucose_1_phosphate + UDP_D_galactose -> UDP_D_glucose + alpha_D_Galactose_1_phosphate |
(( (( (Vmax * A) ) * B) ) / (( (( (Km_A * B) ) + (Km_B * A)) ) + (A * B))) |
|
buffer: 95 mM sodium bicinate, 8 mM cysteine, 0.382 mM NAD+, excess UDP-glucose dehydrogenase
PH: 8.5
Temperature: 27 |
uniprot:P09148 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) wildtype]
UDP_D_glucose + alpha_D_Galactose_1_phosphate -> UDP_D_galactose + alpha_D_Glucose_1_phosphate |
(( (( (Vmax * A) ) * B) ) / (( (( (Km_A * B) ) + (Km_B * A)) ) + (A * B))) |
|
buffer: 95 mM sodium bicinate, 8 mM cysteine, 0.326 mM NADP+, 3.3 µM glucose-1,6-P2, excess phosphoglucomutase, excess glucose-6-P dehydrogenase
PH: 8.5
Temperature: 27 |
uniprot:P09148 [Fe2+]
UDPglucose + alpha_D_Galactose_1_phosphate -> UDPgalactose + D_Glucose_1_phosphate |
(( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (Ka * B) ) + (Kb * A)) ) + (A * B))) |
|
buffer: 95 mM sodium bicinate, 8 mM cysteine, 0.326 mM TPN+, 3.3 µM glucose-1,6-diphosphate, phosphoglucomutase, glucose-6-phosphate dehydrogenase, 10 mM beta-mercaptoethanol
PH: 8.5
Temperature: 27 |
uniprot:P09148 [Fe2+]
alpha_D_Galactose_1_phosphate + UDPglucose -> D_Glucose_1_phosphate + UDPgalactose |
(( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (Ka * B) ) + (Kb * A)) ) + (A * B))) |
|
buffer: 95 mM sodium bicinate, 8 mM cysteine, 0.326 mM TPN+, 3.3 µM glucose-1,6-diphosphate, phosphoglucomutase, glucose-6-phosphate dehydrogenase, 10 mM beta-mercaptoethanol
PH: 8.5
Temperature: 4 |
uniprot:P09148 [Fe2+]
D_Glucose_1_phosphate + UDPgalactose -> UDPglucose + alpha_D_Galactose_1_phosphate |
(( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (Ka * B) ) + (Kb * A)) ) + (A * B))) |
|
buffer: 95 mM sodium bicinate, 8 mM cysteine, 0.382 mM DPN+, UDPglucose dehydrogenase, 10 mM beta-mercaptoethanol
PH: 8.5
Temperature: 27 |
uniprot:P09148 [Fe2+]
D_Glucose_1_phosphate + UDPgalactose -> alpha_D_Galactose_1_phosphate + UDPglucose |
(( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (Ka * B) ) + (Kb * A)) ) + (A * B))) |
|
buffer: 95 mM sodium bicinate, 8 mM cysteine, 0.382 mM DPN+, UDPglucose dehydrogenase, 10 mM beta-mercaptoethanol
PH: 8.5
Temperature: 27 |
uniprot:P09148 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) wildtype]
UDPglucose + alpha_D_Galactose_1_phosphate -> D_Glucose_1_phosphate + UDPgalactose |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 83.33 mM Glycine-NaOH, 5 mM NaF, 14.5 mM Cysteine
PH: 8.5
Temperature: 25 |
uniprot:P09148 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) wildtype]
alpha_D_Galactose_1_phosphate + UDPglucose -> UDPgalactose + D_Glucose_1_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 83.33 mM Glycine-NaOH, 5 mM NaF, 14.5 mM Cysteine
PH: 8.5
Temperature: 25 |
uniprot:P09148 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) wildtype]
alpha_D_Galactose_1_phosphate + UDPglucose -> UDPgalactose + D_Glucose_1_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 83.33 mM Glycine-NaOH, 5 mM NaF, 14.5 mM Cysteine
PH: 8.5
Temperature: 25 |
uniprot:P09148 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) wildtype]
D_Glucose_1_phosphate + UDPgalactose -> alpha_D_Galactose_1_phosphate + UDPglucose |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 58 mM 2-Amino-2-methyl-1,3-propanediol, 0.48 mM NAD+, 25.8 mM Cysteine, 0.048 units/ml UDP-glucose dehydrogenase
PH: 8.5
Temperature: 27 |
uniprot:P09148 [UDP-glucose---hexose-1-phosphate uridylyltransferase(Enzyme) wildtype]
D_Glucose_1_phosphate + UDPgalactose -> UDPglucose + alpha_D_Galactose_1_phosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 58 mM 2-Amino-2-methyl-1,3-propanediol, 0.48 mM NAD+, 25.8 mM Cysteine, 0.048 units/ml UDP-glucose dehydrogenase
PH: 8.5
Temperature: 25 |