EC: 2.7.2.11

glutamate 5-kinase (ATP:L-glutamate 5-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:P32296 [glutamate 5-kinase(Enzyme) wildtype]

ATP + L_Glutamate -> L_Glutamyl_5_phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 20 mM MgCl2, 100 mM hydroxamate-HCl
PH: 7
Temperature: 37
uniprot:P32296 [glutamate 5-kinase(Enzyme) mutant F129A]

L_Glutamate + ATP -> L_Glutamyl_5_phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 20 mM MgCl2, 100 mM hydroxamate-HCl
PH: 7
Temperature: 37
uniprot:P32296 [glutamate 5-kinase(Enzyme) wildtype]

ATP + L_Glutamate -> ADP + L_Glutamyl_5_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 20 mM MgCl2, 100 mM hydroxamate-HCl
PH: 7
Temperature: 37
uniprot:P32296 [glutamate 5-kinase(Enzyme) mutant F129A]

ATP + L_Glutamate -> L_Glutamyl_5_phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris, 20 mM MgCl2, 100 mM hydroxamate-HCl
PH: 7
Temperature: 37
uniprot:A0A0R6Y3I5 [Mg2+]

L_Glutamate + ATP -> ADP + L_Glutamyl_5_phosphate		 (( (E * kcat) ) / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 0.1 M imidazole, 1 mM DTT, 0.1 mg/ml BSA, 50 U/ml pyruvate kinase/lactate dehydrogenase, 1 mM phosphoenolpyruvate, 250 mM NADH
PH: 7.4
Temperature: 25
uniprot:A0A0R6Y3I5 [Mg2+]

L_Glutamate + ATP -> L_Glutamyl_5_phosphate + ADP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.1 M imidazole, 1 mM DTT, 0.1 mg/ml BSA, 50 U/ml pyruvate kinase/lactate dehydrogenase, 1 mM phosphoenolpyruvate, 250 mM NADH
PH: 7.4
Temperature: 25
uniprot:A0A0R6Y3I5 [Mg2+]

L_Glutamate + ATP -> L_Glutamyl_5_phosphate + ADP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.1 M imidazole, 1 mM DTT, 0.1 mg/ml BSA, 50 U/ml pyruvate kinase/lactate dehydrogenase, 1 mM phosphoenolpyruvate, 250 mM NADH
PH: 7.4
Temperature: 25
uniprot:P0A7B5 [glutamate 5-kinase(Enzyme) mutant ProB (3,4-dehydroproline resistant)]

ATP + L_Glutamate -> ADP + L_Glutamyl_5_phosphate		 (( (Vmax * (B ^ n)) ) / (( (B_half ^ n) ) + (B ^ n))) buffer: 50 mM Tris base, 20 mM MgCl2, 0.15 mM NADPH, 0.5 U/ml GP-reductase
PH: 7
Temperature: 22
uniprot:P0A7B5 [glutamate 5-kinase(Enzyme) wildtype]

L_Glutamate + ATP -> ADP + L_Glutamyl_5_phosphate		 (( (Vmax * (B ^ n)) ) / (( (B_half ^ n) ) + (B ^ n))) buffer: 50 mM Tris base, 20 mM MgCl2, 0.15 mM NADPH, 0.5 U/ml GP-reductase
PH: 7
Temperature: 22
uniprot:P0A7B5 [glutamate 5-kinase(Enzyme) wildtype]

L_Glutamate + ATP -> L_Glutamyl_5_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris base, 20 mM MgCl2, 0.15 mM NADPH, 0.5 U/ml GP-reductase
PH: 7
Temperature: 22
uniprot:P0A7B5 [glutamate 5-kinase(Enzyme) mutant ProB (3,4-dehydroproline resistant)]

ATP + L_Glutamate -> ADP + L_Glutamyl_5_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris base, 20 mM MgCl2, 0.15 mM NADPH, 0.5 U/ml GP-reductase
PH: 7
Temperature: 22
uniprot:A0A0R6Y3I5 [L-Proline]

L_Glutamate + ATP -> ADP + L_Glutamyl_5_phosphate		 (( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M imidazole, 1 mM DTT, 0.1 mg/ml BSA, 50 U/ml pyruvate kinase/lactate dehydrogenase, 1 mM phosphoenolpyruvate, 250 mM NADH
PH: 7.4
Temperature: 25

Pathways

pathway id name
BioCyc:HUMAN_PROSYN-PWY proline biosynthesis
BioCyc:META_PWY-5004 superpathway of L-citrulline metabolism
BioCyc:YEAST_CITRULBIO-PWY citrulline biosynthesis
BioCyc:ARA_ARGININE-SYN4-PWY L-ornithine biosynthesis II
BioCyc:ARA_CITRULBIO-PWY L-citrulline biosynthesis
BioCyc:ARA_PROSYN-PWY L-proline biosynthesis I
BioCyc:MTBH37RV_PROSYN-PWY L-proline biosynthesis I
BioCyc:ECOO157_PWY-3341 proline biosynthesis III
BioCyc:ANTHRA_PWY-3341 L-proline biosynthesis III
BioCyc:AGRO_PROSYN-PWY L-proline biosynthesis I
BioCyc:BSUB_PROSYN-PWY L-proline biosynthesis I
BioCyc:SYNEL_PROSYN-PWY L-proline biosynthesis I
BioCyc:CAULONA1000_PROSYN-PWY proline biosynthesis I
BioCyc:META_PWY-6922 L-Nδ-acetylornithine biosynthesis
BioCyc:ARA_PWY-3341 L-proline biosynthesis III
BioCyc:MTBCDC1551_PROSYN-PWY L-proline biosynthesis I
BioCyc:CAULO_PROSYN-PWY proline biosynthesis I
BioCyc:ANTHRA_PROSYN-PWY L-proline biosynthesis I
BioCyc:MOUSE_PWY-5004 superpathway of citrulline metabolism
BioCyc:ECOL316407_PROSYN-PWY proline biosynthesis I
BioCyc:CORYNE_PROSYN-PWY L-proline biosynthesis I
BioCyc:HUMAN_ARGININE-SYN4-PWY ornithine de novo biosynthesis
BioCyc:META_CITRULBIO-PWY L-citrulline biosynthesis
BioCyc:META_PWY-3341 L-proline biosynthesis III
BioCyc:ECO_PROSYN-PWY L-proline biosynthesis I
BioCyc:MOUSE_PROLINE-SYN2-PWY proline biosynthesis II
BioCyc:META_ARGININE-SYN4-PWY L-ornithine biosynthesis II
BioCyc:META_PROSYN-PWY L-proline biosynthesis I
BioCyc:YEAST_PROSYN-PWY proline biosynthesis
BioCyc:ARA_PWY-6922 L-Nδ-acetylornithine biosynthesis
BioCyc:MTBH37RV_PWY-3341 L-proline biosynthesis III
BioCyc:ECOO157_PROSYN-PWY proline biosynthesis I
BioCyc:MOUSE_CITRULBIO-PWY citrulline biosynthesis
BioCyc:BTHE_PROSYN-PWY proline biosynthesis I
PathBank:SMP0000815 Proline Metabolism
Plant Reactome:R-OSA-1119495 Citrulline biosynthesis
Plant Reactome:R-OSA-1119631 Proline biosynthesis I
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Plant Reactome:R-OSA-2744343 Amino acid metabolism
Plant Reactome:R-OSA-5655122 Amino acid biosynthesis
PlantCyc:ARA_CITRULBIO-PWY L-citrulline biosynthesis
PlantCyc:ARA_PROUT-PWY L-proline degradation
PlantCyc:ARA_ARGASEDEG-PWY L-arginine degradation I (arginase pathway)
PlantCyc:ARA_PWY-3341 L-proline biosynthesis III
PlantCyc:ARA_PROSYN-PWY L-proline biosynthesis I
PlantCyc:ARA_ARGININE-SYN4-PWY L-ornithine biosynthesis II
PlantCyc:PLANT_ARGININE-SYN4-PWY L-ornithine biosynthesis II
PlantCyc:ARA_PWY-5004 superpathway of L-citrulline metabolism
PlantCyc:PLANT_PWY-5004 superpathway of L-citrulline metabolism
PlantCyc:PLANT_PWY-6922 L-Nδ-acetylornithine biosynthesis
PlantCyc:ARA_PWY-6922 L-Nδ-acetylornithine biosynthesis
PlantCyc:PLANT_PWY-3341 L-proline biosynthesis III
PlantCyc:PLANT_CITRULBIO-PWY L-citrulline biosynthesis
Reactome:R-CEL-8964539 Glutamate and glutamine metabolism
Reactome:R-MMU-8964539 Glutamate and glutamine metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-CEL-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-CEL-1430728 Metabolism
WikiPathways:WP497 Urea cycle and metabolism of amino groups
WikiPathways:WP5312 Metabolic pathways of fibroblasts
PathBank:SMP0121276 Proline Metabolism
PathBank:SMP0002297 Proline Metabolism