EC: 1.14.16.1

phenylalanine 4-monooxygenase (L-phenylalanine,tetrahydrobiopterin:oxygen oxidoreductase (4-hydroxylating))

enzyme lambda metabolic reaction experiment
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) wildtype hPAH]

L_Phenylalanine + O2 + Tetrahydrobiopterin -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Na-Hepes, 0.04 mg/ml catalase, 5 mM dithiothreitol
PH: 7
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) mutant hPAH(I283C)]

Tetrahydrobiopterin + O2 + L_Phenylalanine -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Na-Hepes, 0.04 mg/ml catalase, 5 mM dithiothreitol
PH: 7
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) mutant hPAH(I283C)]

Tetrahydrobiopterin + L_Phenylalanine + O2 -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (Vmax * Co) ) / (Km + Co)) buffer: 100 mM Na-Hepes, 0.04 mg/ml catalase, 5 mM dithiothreitol
PH: 7
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) wildtype hPAH]

O2 + L_Phenylalanine + Tetrahydrobiopterin -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * Co) ) / (Km + Co)) buffer: 100 mM Na-Hepes, 0.04 mg/ml catalase, 5 mM dithiothreitol
PH: 7
Temperature: 25
uniprot:Q54XS1 [phenylalanine 4-monooxygenase(Enzyme) wildtype His6-tagged dPAH]

Tetrahydrobiopterin + L_Phenylalanine + O2 -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * Co) ) / (Km + Co)) buffer: 100 mM Na-Hepes, 0.04 mg/ml catalase, 5 mM dithiothreitol
PH: 7
Temperature: 25
uniprot:Q54XS1 [phenylalanine 4-monooxygenase(Enzyme) wildtype His6-tagged dPAH]

Tetrahydrobiopterin + L_Phenylalanine + O2 -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Na-Hepes, 0.04 mg/ml catalase, 5 mM dithiothreitol
PH: 7
Temperature: 25
uniprot:Q54XS1 [phenylalanine 4-monooxygenase(Enzyme) wildtype His6-tagged dPAH]

O2 + L_Phenylalanine + Tetrahydrodictyopterin -> L_Tyrosine + 4a_Hydroxytetrahydrodictyopterin		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Na-Hepes, 0.04 mg/ml catalase, 5 mM dithiothreitol
PH: 7
Temperature: 25
uniprot:Q54XS1 [phenylalanine 4-monooxygenase(Enzyme) wildtype His6-tagged dPAH]

O2 + L_Phenylalanine + Tetrahydrodictyopterin -> L_Tyrosine + 4a_Hydroxytetrahydrodictyopterin		 (( (Vmax * Co) ) / (Km + Co)) buffer: 100 mM Na-Hepes, 0.04 mg/ml catalase, 5 mM dithiothreitol
PH: 7
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype delta117PheH]

L_Phenylalanine + O2 + 6_Methyltetrahydropterin -> L_Tyrosine + H2O + 6_Methyldihydropterin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Hepes/NaOH, 5 mM DTT, 15 µM ferrous ammonium sulfate, 60 µg/ml catalase
PH: 7
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype delta117PheH]

L_Phenylalanine + 6_Methyltetrahydropterin + O2 -> H2O + L_Tyrosine + 6_Methyldihydropterin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Hepes/NaOH, 5 mM DTT, 15 µM ferrous ammonium sulfate, 60 µg/ml catalase
PH: 7
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant delta117PheH V379D]

O2 + 6_Methyltetrahydropterin + L_Phenylalanine -> L_Tyrosine + H2O + 6_Methyldihydropterin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Hepes/NaOH, 5 mM DTT, 15 µM ferrous ammonium sulfate, 60 µg/ml catalase
PH: 7
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype delta117PheH]

L_Phenylalanine + 6_Methyltetrahydropterin + O2 -> 6_Methyldihydropterin + H2O + L_Tyrosine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Hepes/NaOH, 15 µM ferrous ammonium sulfate, 60 µg/ml catalase, 200-250 µM NADH, 0.2-0.5 U/ml dihydropteridine reductase
PH: 7
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant delta117PheH V379D]

6_Methyltetrahydropterin + L_Phenylalanine + O2 -> L_Tyrosine + H2O + 6_Methyldihydropterin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Hepes/NaOH, 15 µM ferrous ammonium sulfate, 60 µg/ml catalase, 200-250 µM NADH, 0.2-0.5 U/ml dihydropteridine reductase
PH: 7
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant delta117PheH V379D]

L_Phenylalanine + O2 + 6_Methyltetrahydropterin -> H2O + 6_Methyldihydropterin + L_Tyrosine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Hepes/NaOH, 5 mM DTT, 15 µM ferrous ammonium sulfate, 60 µg/ml catalase
PH: 7
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype delta117PheH]

L_Tryptophan + O2 + 6_Methyltetrahydropterin -> 6_Methyldihydropterin + 5_Hydroxytryptophan + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Hepes/NaOH, 7 mM DTT, 10 µM ferrous ammonium sulfate, 50 µg/ml catalase
PH: 7
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype delta117PheH]

6_Methyltetrahydropterin + L_Tryptophan + O2 -> H2O + 5_Hydroxytryptophan + 6_Methyldihydropterin		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 80 mM Hepes/NaOH, 15 µM ferrous ammonium sulfate, 60 µg/ml catalase, 200-250 µM NADH, 0.2-0.5 U/ml dihydropteridine reductase
PH: 7
Temperature: 25
uniprot:P00439 [1,4-Dithiothreitol]

O2 + L_Phenylalanine + Tetrahydrobiopterin -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M KPO4, 0.15 M KCl, 0.06 units/µl Bovine liver catalase
PH: 6.8
Temperature: 22
uniprot:P00439 [1,4-Dithiothreitol]

O2 + L_Phenylalanine + Tetrahydrobiopterin -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M KPO4, 0.15 M KCl, 0.06 units/µl Bovine liver catalase
PH: 6.8
Temperature: 22
uniprot:P00439 [1,4-Dithiothreitol]

Tetrahydrobiopterin + L_Phenylalanine + O2 -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M KPO4, 0.15 M KCl, 0.06 units/µl Bovine liver catalase
PH: 6.8
Temperature: 22
uniprot:P00439 [1,4-Dithiothreitol]

O2 + Tetrahydrobiopterin + L_Phenylalanine -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M KPO4, 0.15 M KCl, 0.06 units/µl Bovine liver catalase
PH: 6.8
Temperature: 22
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

6_Methyltetrahydropterin + L_Phenylalanine + O2 -> L_Tyrosine + H2O + 6_Methyldihydropterin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M KPO4, 0.15 M KCl, 0.06 units/µl Bovine liver catalase
PH: 6.8
Temperature: 22
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

6_Methyltetrahydropterin + O2 + L_Phenylalanine -> L_Tyrosine + H2O + 6_Methyldihydropterin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M KPO4, 0.15 M KCl, 0.06 units/µl Bovine liver catalase
PH: 6.8
Temperature: 22
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

6_Methyltetrahydropterin + L_Phenylalanine + O2 -> 6_Methyldihydropterin + H2O + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M KPO4, 0.15 M KCl, 0.06 units/µl Bovine liver catalase
PH: 6.8
Temperature: 22
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

O2 + L_Phenylalanine + 6_Methyltetrahydropterin -> 6_Methyldihydropterin + H2O + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M KPO4, 0.15 M KCl, 0.06 units/µl Bovine liver catalase
PH: 6.8
Temperature: 22
uniprot:P00439 [L-Ascorbate]

L_Phenylalanine + O2 + Tetrahydrobiopterin -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M KPO4, 0.15 M KCl, 0.06 units/µl Bovine liver catalase
PH: 6.8
Temperature: 22
uniprot:P00439 [2-Mercaptoethanol]

O2 + Tetrahydrobiopterin + L_Phenylalanine -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M KPO4, 0.15 M KCl, 0.06 units/µl Bovine liver catalase
PH: 6.8
Temperature: 22
uniprot:P00439 [Thioglycerol]

L_Phenylalanine + O2 + Tetrahydrobiopterin -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M KPO4, 0.15 M KCl, 0.06 units/µl Bovine liver catalase
PH: 6.8
Temperature: 22
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant H264Q/V379D]

6_Methyltetrahydropterin + L_Phenylalanine + O2 -> H2O + L_Tyrosine + 6_Methyldihydropterin		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 10 µM Ferrous ammonium sulfate, 1 mM DTT
PH: 6.7
Temperature: 32
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

O2 + 6_Methyltetrahydropterin + L_Phenylalanine -> L_Tyrosine + H2O + 6_Methyldihydropterin		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 10 µM Ferrous ammonium sulfate, 1 mM DTT
PH: 6.7
Temperature: 32
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant Y277H]

O2 + L_Phenylalanine + 6_Methyltetrahydropterin -> 6_Methyldihydropterin + L_Tyrosine + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 10 µM Ferrous ammonium sulfate, 1 mM DTT
PH: 6.7
Temperature: 32
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant V379D]

O2 + 6_Methyltetrahydropterin + L_Phenylalanine -> 6_Methyldihydropterin + H2O + L_Tyrosine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 10 µM Ferrous ammonium sulfate, 1 mM DTT
PH: 6.7
Temperature: 32
uniprot:P04177 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

O2 + L_Phenylalanine + 6_Methyltetrahydropterin -> 6_Methyldihydropterin + L_Tyrosine + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 10 µM Ferrous ammonium sulfate, 1 mM DTT
PH: 6.7
Temperature: 32
uniprot:P04177 [phenylalanine 4-monooxygenase(Enzyme) mutant D425V]

L_Phenylalanine + 6_Methyltetrahydropterin + O2 -> 6_Methyldihydropterin + H2O + L_Tyrosine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 10 µM Ferrous ammonium sulfate, 1 mM DTT
PH: 6.7
Temperature: 32
uniprot:P04177 [phenylalanine 4-monooxygenase(Enzyme) mutant H323Y]

L_Phenylalanine + 6_Methyltetrahydropterin + O2 -> L_Tyrosine + 6_Methyldihydropterin + H2O		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 10 µM Ferrous ammonium sulfate, 1 mM DTT
PH: 6.7
Temperature: 32
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant H264Q]

L_Phenylalanine + O2 + 6_Methyltetrahydropterin -> H2O + L_Tyrosine + 6_Methyldihydropterin		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 10 µM Ferrous ammonium sulfate, 1 mM DTT
PH: 6.7
Temperature: 32
uniprot:P04177 [phenylalanine 4-monooxygenase(Enzyme) mutant Q310H]

O2 + L_Phenylalanine + 6_Methyltetrahydropterin -> L_Tyrosine + H2O + 6_Methyldihydropterin		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 10 µM Ferrous ammonium sulfate, 1 mM DTT
PH: 6.7
Temperature: 32
uniprot:P00439 [Fe2+]

L_Phenylalanine + O2 + Tetrahydrobiopterin -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M Hepes-Na, 1 mg/ml Catalase, 5 mM DTT
PH: 7
Temperature: 25
uniprot:P00439 [Fe2+]

L_Phenylalanine + O2 + Tetrahydrobiopterin -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M Hepes-Na, 1 mg/ml Catalase, 5 mM DTT
PH: 7
Temperature: 25
uniprot:P04176 [alpha-Tocopherol phosphate]

L_Phenylalanine + Tetrahydrobiopterin + O2 -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Potassium phosphate
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

L_Phenylalanine + O2 + Tetrahydrobiopterin -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M Potassium phosphate
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

O2 + Tetrahydrobiopterin + L_Phenylalanine -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Potassium phosphate
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

L_Phenylalanine + Tetrahydrobiopterin + O2 -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Potassium phosphate
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

L_Phenylalanine + 6_Methyltetrahydropterin + O2 -> L_Tyrosine + 6_Methyldihydropterin + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Potassium phosphate
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

6_Methyltetrahydropterin + O2 + L_Phenylalanine -> L_Tyrosine + 6_Methyldihydropterin + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Potassium phosphate
PH: 6.8
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) mutant PAHdelta116]

L_Tryptophan + O2 + Tetrahydrobiopterin -> 5_Hydroxy_L_tryptophan + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tris-acetate
PH: 7
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant H264Q/Y277H/V379D]

6_Methyltetrahydropterin + O2 + L_Phenylalanine -> L_Tyrosine + H2O + 6_Methyldihydropterin		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES/NaOH, 100 µg/ml Catalase, 10 µM Ferrous ammonium sulfate, 1 mM DTT
PH: 6.7
Temperature: 32
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

Tetrahydrobiopterin + O2 + L_Phenylalanine -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Potassium phosphate
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

O2 + Tetrahydrobiopterin + L_Phenylalanine -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Potassium phosphate
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

O2 + L_Phenylalanine + 6,7_Dimethyl_tetrahydropteridine -> L_Tyrosine + 6,7_Dimethyl_dihydropteridine + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Potassium phosphate
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

6,7_Dimethyl_tetrahydropteridine + O2 + L_Phenylalanine -> 6,7_Dimethyl_dihydropteridine + H2O + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Potassium phosphate
PH: 6.8
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

Tetrahydrobiopterin + L_Phenylalanine + O2 -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM HEPES, 60 µM Dithiothreitol, 50 µg/ml Catalase
PH: 7
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) mutant PAHdelta116]

O2 + L_Phenylalanine + Tetrahydrobiopterin -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM HEPES, 60 µM Dithiothreitol, 50 µg/ml Catalase
PH: 7
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

L_Phenylalanine + 6_Methyltetrahydropterin + O2 -> H2O + 6_Methyldihydropterin + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM HEPES, 60 µM Dithiothreitol, 50 µg/ml Catalase
PH: 7
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) mutant PAHdelta116]

6_Methyltetrahydropterin + O2 + L_Phenylalanine -> H2O + 6_Methyldihydropterin + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM HEPES, 60 µM Dithiothreitol, 50 µg/ml Catalase
PH: 7
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) mutant PAHdelta116]

L_Phenylalanine + 6_Methyltetrahydropterin + O2 -> H2O + L_Tyrosine + 6_Methyldihydropterin		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM HEPES, 60 µM Dithiothreitol, 50 µg/ml Catalase
PH: 7
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

L_Phenylalanine + 6_Methyltetrahydropterin + O2 -> 6_Methyldihydropterin + L_Tyrosine + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 80 mM HEPES, 60 µM Dithiothreitol, 50 µg/ml Catalase
PH: 7
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) mutant PAHdelta116]

O2 + Tetrahydrobiopterin + L_Tryptophan -> 5_Hydroxy_L_tryptophan + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tris-acetate
PH: 8
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

Tetrahydrobiopterin + L_Phenylalanine + O2 -> L_Tyrosine + H2O + 6,7_Dihydrobiopterin		 (( (Vmax + (Vi * (( (S ^ x) ) / (Ki ^ x)))) ) / (( (1 + (( (S05 ^ h) ) / (S ^ h))) ) + (( (S ^ x) ) / (Ki ^ x)))) buffer: 0.1 M Na-HEPES, 0.1 µg/µl Catalase, 100 µM Ferrous Ammonium Sulphate, 5 mM DTT, 0.5 % BSA
PH: 7
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

L_Phenylalanine + O2 + Tetrahydrobiopterin -> H2O + L_Tyrosine + 6,7_Dihydrobiopterin		 (( (Vmax * (S ^ h)) ) / (( (S ^ h) ) + (S05 ^ h))) buffer: 0.1 M Na-HEPES, 0.1 µg/µl Catalase, 100 µM Ferrous Ammonium Sulphate, 5 mM DTT, 0.5 % BSA
PH: 7
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

Tetrahydrobiopterin + L_Phenylalanine + O2 -> H2O + 6,7_Dihydrobiopterin + L_Tyrosine		 (( (Vmax * (S ^ h)) ) / (( (S ^ h) ) + (S05 ^ h))) buffer: 0.1 M Na-HEPES, 0.1 µg/µl Catalase, 100 µM Ferrous Ammonium Sulphate, 5 mM DTT, 0.5 % BSA
PH: 7
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) mutant T427P]

O2 + L_Phenylalanine + Tetrahydrobiopterin -> 6,7_Dihydrobiopterin + H2O + L_Tyrosine		 (( (Vmax * (S ^ h)) ) / (( (S ^ h) ) + (S05 ^ h))) buffer: 0.1 M Na-HEPES, 0.1 µg/µl Catalase, 100 µM Ferrous Ammonium Sulphate, 5 mM DTT, 0.5 % BSA
PH: 7
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) mutant T427P]

Tetrahydrobiopterin + O2 + L_Phenylalanine -> L_Tyrosine + H2O + 6,7_Dihydrobiopterin		 (( (Vmax * (S ^ h)) ) / (( (S ^ h) ) + (S05 ^ h))) buffer: 0.1 M Na-HEPES, 0.1 µg/µl Catalase, 100 µM Ferrous Ammonium Sulphate, 5 mM DTT, 0.5 % BSA
PH: 7
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

L_Phenylalanine + O2 + Tetrahydrobiopterin -> 6,7_Dihydrobiopterin + L_Tyrosine + H2O		 (( (Vmax * T) ) / (Km + T)) buffer: 0.1 M Na-HEPES, 0.1 µg/µl Catalase, 100 µM Ferrous Ammonium Sulphate, 5 mM DTT, 0.5 % BSA
PH: 7
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

L_Phenylalanine + Tetrahydrobiopterin + O2 -> H2O + L_Tyrosine + 6,7_Dihydrobiopterin		 (( (Vmax * T) ) / (Km + T)) buffer: 0.1 M Na-HEPES, 0.1 µg/µl Catalase, 100 µM Ferrous Ammonium Sulphate, 5 mM DTT, 0.5 % BSA
PH: 7
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) mutant T427P]

Tetrahydrobiopterin + L_Phenylalanine + O2 -> H2O + L_Tyrosine + 6,7_Dihydrobiopterin		 (( (Vmax * T) ) / (Km + T)) buffer: 0.1 M Na-HEPES, 0.1 µg/µl Catalase, 100 µM Ferrous Ammonium Sulphate, 5 mM DTT, 0.5 % BSA
PH: 7
Temperature: 25
uniprot:P00439 [phenylalanine 4-monooxygenase(Enzyme) mutant T427P]

Tetrahydrobiopterin + L_Phenylalanine + O2 -> H2O + 6,7_Dihydrobiopterin + L_Tyrosine		 (( (Vmax * T) ) / (Km + T)) buffer: 0.1 M Na-HEPES, 0.1 µg/µl Catalase, 100 µM Ferrous Ammonium Sulphate, 5 mM DTT, 0.5 % BSA
PH: 7
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

Tetrahydrobiopterin + O2 + L_Phenylalanine -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 0.01 % catalase, 0.125 mM NADH, sheep liver dihydropteridine reductase
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant S16E]

O2 + L_Phenylalanine + Tetrahydrobiopterin -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 0.01 % catalase, 0.125 mM NADH, sheep liver dihydropteridine reductase
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype S16 phosphorylated]

Tetrahydrobiopterin + O2 + L_Phenylalanine -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 0.01 % catalase, 0.125 mM NADH, sheep liver dihydropteridine reductase
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant S16Q]

Tetrahydrobiopterin + O2 + L_Phenylalanine -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 0.01 % catalase, 0.125 mM NADH, sheep liver dihydropteridine reductase
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant S16A]

O2 + L_Phenylalanine + Tetrahydrobiopterin -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 0.01 % catalase, 0.125 mM NADH, sheep liver dihydropteridine reductase
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant S16N]

L_Phenylalanine + O2 + Tetrahydrobiopterin -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 0.01 % catalase, 0.125 mM NADH, sheep liver dihydropteridine reductase
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant S16K]

O2 + Tetrahydrobiopterin + L_Phenylalanine -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 0.01 % catalase, 0.125 mM NADH, sheep liver dihydropteridine reductase
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant S16Q]

L_Phenylalanine + O2 + Tetrahydrobiopterin -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 0.01 % catalase, 0.125 mM NADH, sheep liver dihydropteridine reductase
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant S16D]

L_Phenylalanine + O2 + Tetrahydrobiopterin -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 0.01 % catalase, 0.125 mM NADH, sheep liver dihydropteridine reductase
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant S16E]

Tetrahydrobiopterin + O2 + L_Phenylalanine -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 0.01 % catalase, 0.125 mM NADH, sheep liver dihydropteridine reductase
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype]

O2 + Tetrahydrobiopterin + L_Phenylalanine -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 0.01 % catalase, 0.125 mM NADH, sheep liver dihydropteridine reductase
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) wildtype S16 phosphorylated]

L_Phenylalanine + O2 + Tetrahydrobiopterin -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 0.01 % catalase, 0.125 mM NADH, sheep liver dihydropteridine reductase
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant S16N]

Tetrahydrobiopterin + O2 + L_Phenylalanine -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 0.01 % catalase, 0.125 mM NADH, sheep liver dihydropteridine reductase
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant S16K]

O2 + L_Phenylalanine + Tetrahydrobiopterin -> 4a_Hydroxytetrahydrobiopterin + L_Tyrosine		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 0.01 % catalase, 0.125 mM NADH, sheep liver dihydropteridine reductase
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant S16D]

L_Phenylalanine + O2 + Tetrahydrobiopterin -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 0.01 % catalase, 0.125 mM NADH, sheep liver dihydropteridine reductase
PH: 6.8
Temperature: 25
uniprot:P04176 [phenylalanine 4-monooxygenase(Enzyme) mutant S16A]

L_Phenylalanine + O2 + Tetrahydrobiopterin -> L_Tyrosine + 4a_Hydroxytetrahydrobiopterin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 0.01 % catalase, 0.125 mM NADH, sheep liver dihydropteridine reductase
PH: 6.8
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY-6134 L-tyrosine biosynthesis IV
BioCyc:CAULO_PHENYLALANINE-DEG1-PWY phenylalanine degradation I (aerobic)
BioCyc:MOUSE_PHENYLALANINE-DEG1-PWY phenylalanine degradation I (aerobic)
BioCyc:HUMAN_PHENYLALANINE-DEG1-PWY phenylalanine degradation/tyrosine biosynthesis
BioCyc:META_PHENYLALANINE-DEG1-PWY L-phenylalanine degradation I (aerobic)
BioCyc:VCHO_PHENYLALANINE-DEG1-PWY L-phenylalanine degradation I (aerobic)
BioCyc:CAULO_PWY-6134 tyrosine biosynthesis IV
BioCyc:META_PWY-7158 L-phenylalanine degradation V
BioCyc:MOUSE_TYRSYN2 tyrosine biosynthesis II
PlantCyc:CHLAMY_PWY-7158 L-phenylalanine degradation V
PlantCyc:MOSS_PWY-7158 L-phenylalanine degradation V
PlantCyc:PLANT_PWY-7158 L-phenylalanine degradation V
Reactome:R-BTA-8964208 Phenylalanine metabolism
Reactome:R-DME-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-HSA-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-RNO-8964208 Phenylalanine metabolism
Reactome:R-BTA-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-CEL-8964208 Phenylalanine metabolism
Reactome:R-RNO-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-DDI-8964208 Phenylalanine metabolism
Reactome:R-MMU-8964208 Phenylalanine metabolism
Reactome:R-CEL-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-DME-8964208 Phenylalanine metabolism
Reactome:R-DDI-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-MMU-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-HSA-8964208 Phenylalanine metabolism
Reactome:R-DDI-209905 Catecholamine biosynthesis
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-1643685 Disease
Reactome:R-MMU-1430728 Metabolism
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-DDI-209776 Metabolism of amine-derived hormones
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-71291 Amino acid and derivative metabolism
Reactome:R-CEL-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-5668914 Diseases of metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-209931 Serotonin and melatonin biosynthesis
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2160456 Phenylketonuria
WikiPathways:WP4156 Biosynthesis and regeneration of tetrahydrobiopterin and catabolism of phenylalanine
PathBank:SMP0000369 Tyrosinemia Type 2 (or Richner-Hanhart Syndrome)
PathBank:SMP0120733 Tyrosinemia Type 2 (or Richner-Hanhart Syndrome)
PathBank:SMP0000206 Phenylketonuria
PathBank:SMP0087313 Phenylalanine and Tyrosine Metabolism
PathBank:SMP0120760 Phenylketonuria
PathBank:SMP0000370 Tyrosinemia Type 3 (TYRO3)
PathBank:SMP0120731 Tyrosinemia Type 3 (TYRO3)
PathBank:SMP0000008 Phenylalanine and Tyrosine Metabolism
PathBank:SMP0087451 Tryptophan Metabolism