EC: 7.1.1.1

proton-translocating NAD(P)+ transhydrogenase (NADPH:NAD+ oxidoreductase (H+-transporting))

enzyme lambda metabolic reaction experiment
uniprot:Q5BJZ3 [proton-translocating NAD(P)+ transhydrogenase(Enzyme) wildtype]

NADP+ + NADH -> NADPH + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02 % Triton X-100, 1 mM GSSG, 0.5-1 U/ml glutathione reductase, 5 µM rotenone, 0.1-0.2 mM DTNB
PH: 7.2
Temperature: 37
uniprot:Q5BJZ3 [proton-translocating NAD(P)+ transhydrogenase(Enzyme) wildtype]

NADH + NADP+ -> NADPH + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02 % Triton X-100, 1 mM GSSG, 0.5-1 U/ml glutathione reductase, 5 µM rotenone, 0.1-0.2 mM DTNB
PH: 7.2
Temperature: 37
uniprot:Q5BJZ3 [proton-translocating NAD(P)+ transhydrogenase(Enzyme) wildtype]

NADP+ + NADH -> NADPH + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM MOPS, 120 mM KCl, 1 mM EGTA, 0.02 % Triton X-100, 1 mM GSSG, 0.5-1 U/ml glutathione reductase, 5 µM rotenone, 0.1-0.2 mM DTNB
PH: 7.2
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_NADPHOS-DEPHOS-PWY-1 NAD phosphorylation and dephosphorylation
BioCyc:META_NADPHOS-DEPHOS-PWY-1 NAD phosphorylation and transhydrogenation
BioCyc:META_NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation
BioCyc:ECOL316407_NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation I
BioCyc:ECO_NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation
BioCyc:ECOO157_NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation I
PathBank:SMP0002093 NAD Phosphorylation and Dephosphorylation
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-BTA-71403 Citric acid cycle (TCA cycle)
Reactome:R-MMU-1430728 Metabolism
Reactome:R-HSA-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-HSA-71403 Citric acid cycle (TCA cycle)
Reactome:R-MMU-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-MMU-71403 Citric acid cycle (TCA cycle)
Reactome:R-HSA-1430728 Metabolism
PathBank:SMP0000048 Nicotinate and Nicotinamide Metabolism