EC: 6.3.2.8

UDP-N-acetylmuramate-L-alanine ligase (UDP-N-acetylmuramate:L-alanine ligase (ADP-forming))

enzyme lambda metabolic reaction experiment
uniprot:P17952 [Ammonium sulfate]

ATP + UDP_N_acetylmuramate + L_Alanine -> Phosphate + ADP + UDP_N_acetylmuramoyl_L_alanine		 (( (Vmax * C) ) / (Km + C)) buffer: 50 mM Tris-HCl, 1 mM DTT, 2 mM PEP, 0.4 mM NADH, 0.9 U/ml pyruvate kinase, 0.9 U/ml lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P17952 [Ammonium sulfate]

L_Alanine + UDP_N_acetylmuramate + ATP -> Phosphate + UDP_N_acetylmuramoyl_L_alanine + ADP		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 1 mM DTT, 2 mM PEP, 0.4 mM NADH, 0.9 U/ml pyruvate kinase, 0.9 U/ml lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P17952 [Ammonium sulfate]

UDP_N_acetylmuramate + L_Alanine + ATP -> Phosphate + UDP_N_acetylmuramoyl_L_alanine + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mM DTT, 2 mM PEP, 0.4 mM NADH, 0.9 U/ml pyruvate kinase, 0.9 U/ml lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P17952 [Ammonium sulfate]

ATP + UDP_N_acetylmuramate + L_Serine -> UDP_N_acetylmuramoyl_L_serine + ADP + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mM DTT, 2 mM PEP, 0.4 mM NADH, 0.9 U/ml pyruvate kinase, 0.9 U/ml lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P17952 [Ammonium sulfate]

Glycine + UDP_N_acetylmuramate + ATP -> ADP + UDP_N_acetylmuramoyl_glycine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mM DTT, 2 mM PEP, 0.4 mM NADH, 0.9 U/ml pyruvate kinase, 0.9 U/ml lactate dehydrogenase
PH: 8
Temperature: 37
uniprot:P17952 [Ammonium sulfate]

UDP_N_acetylmuramate + 3_Chloro_L_alanine + ATP -> Phosphate + UDP_N_acetylmuramoyl_L_3_chloro_L_alanine + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mM DTT, 2 mM PEP, 0.4 mM NADH, 0.9 U/ml pyruvate kinase, 0.9 U/ml lactate dehydrogenase
PH: 8
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:ECO_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:HPY_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-DAP-containing)
BioCyc:CAULO_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-DAP-containing)
BioCyc:BSUB_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:SYNEL_PWY0-1261 anhydromuropeptides recycling
BioCyc:GCF_000013425_PWY-6470 peptidoglycan biosynthesis V (β-lactam resistance)
BioCyc:GCF_000013425_PWY-6386 UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing)
BioCyc:META_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:META_PWY-5265 peptidoglycan biosynthesis II (staphylococci)
BioCyc:MTBCDC1551_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:ECOL199310_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-DAP-containing)
BioCyc:ANTHRA_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:ANTHRA_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:AGRO_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:META_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:ECO_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:MTBCDC1551_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:AGRO_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:SYNEL_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:SYNEL_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:ECOL316407_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:CAULONA1000_PWY-5746 peptidoglycan biosynthesis I
BioCyc:PDIF272563_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:PDIF272563_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:CORYNE_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:META_PWY-6386 UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing)
BioCyc:MTBH37RV_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:MTBH37RV_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:HPY_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:ECOL199310_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:ECOL199310_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:CAULO_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:BSUB_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:ECOL316407_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-DAP-containing)
BioCyc:CORYNE_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:GCF_000013425_PWY-5265 peptidoglycan biosynthesis II (staphylococci)
PathBank:SMP0000792 D-Glutamine and D-Glutamate Metabolism
PathBank:SMP0000924 Peptidoglycan Biosynthesis I
PathBank:SMP0002074 Peptidoglycan Biosynthesis II
PathBank:SMP0122239 Peptidoglycan Biosynthesis II
PathBank:SMP0121265 D-Glutamine and D-Glutamate Metabolism
PathBank:SMP0121305 Peptidoglycan Biosynthesis I