EC: 6.2.1.5

succinate-CoA ligase (ADP-forming) (succinate:CoA ligase (ADP-forming))

enzyme lambda metabolic reaction experiment
- [succinate---CoA ligase (ADP-forming)(Enzyme) wildtype isoform A-SCS]

Coenzyme_A + Succinate + ATP -> ADP + Succinyl_CoA + Phosphate		 (( (Vmax * A) ) / (( (Km * (1 + (A / Kis))) ) + A)) buffer: 100 mM Tris acetate, 7.1 mM Magnesium acetate, 10 mM KCl, 1.55 mM Phosphoenolpyruvate, 0.1 mM NADH, 7.5 U/ml pyruvate kinase, 7.5 U/ml lactate dehydrogenase
PH: 8
Temperature: 30
- [succinate---CoA ligase (ADP-forming)(Enzyme) wildtype isoform A-SCS]

Phosphate + Succinyl_CoA + ADP -> ATP + Coenzyme_A + Succinate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris acetate, 7.1 mM Magnesium acetate, 10 mM KCl, 1 mM Glucose, 0.1 mM NADP+, 5 U/ml hexokinase, 5 U/ml glucose-6-phosphate dehydrogenase
PH: 8
Temperature: 30
- [succinate---CoA ligase (ADP-forming)(Enzyme) wildtype isoform A-SCS]

Coenzyme_A + ATP + Succinate -> ADP + Phosphate + Succinyl_CoA		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris acetate, 7.1 mM Magnesium acetate, 10 mM KCl, 1.55 mM Phosphoenolpyruvate, 0.1 mM NADH, 7.5 U/ml pyruvate kinase, 7.5 U/ml lactate dehydrogenase
PH: 8
Temperature: 30
- [succinate---CoA ligase (ADP-forming)(Enzyme) wildtype isoform A-SCS]

Phosphate + Succinyl_CoA + ADP -> Succinate + ATP + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris acetate, 7.1 mM Magnesium acetate, 10 mM KCl, 1 mM Glucose, 0.1 mM NADP+, 5 U/ml hexokinase, 5 U/ml glucose-6-phosphate dehydrogenase
PH: 8
Temperature: 30
- [succinate---CoA ligase (ADP-forming)(Enzyme) wildtype isoform A-SCS]

Succinyl_CoA + ADP + Phosphate -> Coenzyme_A + Succinate + ATP		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris acetate, 7.1 mM Magnesium acetate, 10 mM KCl, 1 mM Glucose, 0.1 mM NADP+, 5 U/ml hexokinase, 5 U/ml glucose-6-phosphate dehydrogenase
PH: 8
Temperature: 30
- [succinate---CoA ligase (ADP-forming)(Enzyme) wildtype isoform A-SCS]

Succinate + ATP + Coenzyme_A -> Succinyl_CoA + Phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris acetate, 7.1 mM Magnesium acetate, 10 mM KCl, 1.55 mM Phosphoenolpyruvate, 0.1 mM NADH, 7.5 U/ml pyruvate kinase, 7.5 U/ml lactate dehydrogenase
PH: 8
Temperature: 30

Pathways

pathway id name
BioCyc:META_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
BioCyc:ECO_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
BioCyc:ARA_PWYQT-4481 TCA cycle variation V (plant)
BioCyc:VCHO_PWY-5690 TCA cycle II (plants and fungi)
BioCyc:MTBCDC1551_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:ECOO157_TCA TCA cycle I (prokaryotic)
BioCyc:ECOO157_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:ECOL199310_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
BioCyc:CAULO_TCA TCA cycle I (prokaryotic)
BioCyc:CAULO_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
BioCyc:MOUSE_TCA-1 TCA cycle
BioCyc:ECOL316407_TCA TCA cycle I (prokaryotic)
BioCyc:CAULONA1000_PWY-5538 pyruvate fermentation to acetate VI
BioCyc:META_PWY-5538 pyruvate fermentation to acetate VI
BioCyc:META_TCA TCA cycle I (prokaryotic)
BioCyc:VCHO_TCA TCA cycle I (prokaryotic)
BioCyc:MTBH37RV_PWY-6969 TCA cycle V (2-oxoglutarate:ferredoxin oxidoreductase)
BioCyc:MTBCDC1551_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
BioCyc:ECOO157_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
BioCyc:ECOL199310_TCA TCA cycle I (prokaryotic)
BioCyc:ECOL199310_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:ANTHRA_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
BioCyc:AGRO_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:AGRO_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
BioCyc:MOUSE_PWY-5690-1 TCA cycle variation III (eukaryotic)
BioCyc:MOUSE_GLUTOXA-PWY glutamate degradation VII
BioCyc:BSUB_TCA TCA cycle I (prokaryotic)
BioCyc:CAULONA1000_PWY-5913 TCA cycle variation IV
BioCyc:BTHE_TCA TCA cycle I (prokaryotic)
BioCyc:GCF_000013425_TCA TCA cycle I (prokaryotic)
BioCyc:HUMAN_PWY66-407 superpathway of conversion of glucose to acetyl CoA and entry into the TCA cycle
BioCyc:HUMAN_PWY66-398 TCA cycle
BioCyc:META_PWY66-398 TCA cycle III (animals)
BioCyc:META_PWY-6969 TCA cycle V (2-oxoglutarate:ferredoxin oxidoreductase)
BioCyc:ECO_TCA TCA cycle I (prokaryotic)
BioCyc:ECO_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:VCHO_PWY-6969 TCA cycle V (2-oxoglutarate:ferredoxin oxidoreductase)
BioCyc:VCHO_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
BioCyc:MTBCDC1551_TCA TCA cycle I (prokaryotic)
BioCyc:ANTHRA_PWY-1622 formaldehyde assimilation I (serine pathway)
BioCyc:AGRO_TCA TCA cycle I (prokaryotic)
BioCyc:ECOL316407_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:CAULONA1000_PWY-5537 pyruvate fermentation to acetate V
BioCyc:CORYNE_TCA TCA cycle I (prokaryotic)
BioCyc:META_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:YEAST_PWY-5690 TCA cycle II (plants and fungi)
BioCyc:ARA_PWY-5690 TCA cycle II (plants and fungi)
BioCyc:VCHO_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:MTBCDC1551_PWY-6969 TCA cycle V (2-oxoglutarate:ferredoxin oxidoreductase)
BioCyc:ANTHRA_TCA TCA cycle I (prokaryotic)
BioCyc:ECOL316407_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
BioCyc:CORYNE_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
WikiPathways:WP2472 Escherichia coli K-12 peripherome
PathBank:SMP0000802 TCA Cycle
PathBank:SMP0001018 TCA cycle (ubiquinol-2)
PathBank:SMP0001019 TCA cycle (ubiquinol-3)
PathBank:SMP0001020 TCA cycle (ubiquinol-4)
PathBank:SMP0001021 TCA cycle (ubiquinol-5)
PathBank:SMP0001022 TCA cycle (ubiquinol-6)
PathBank:SMP0001023 TCA cycle (ubiquinol-7)
PathBank:SMP0001024 TCA cycle (ubiquinol-8)
PathBank:SMP0001025 TCA cycle (ubiquinol-9)
PathBank:SMP0001026 TCA cycle (ubiquinol-10)
PathBank:SMP0002037 TCA cycle (ubiquinol-0)
Plant Reactome:R-OSA-1119533 TCA cycle (plant)
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Plant Reactome:R-OSA-2961031 Generation of precursor metabolites and energy
PlantCyc:ARA_PWY-5464 superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle
PlantCyc:ARA_PWYQT-4481 TCA cycle variation V (plant)
PlantCyc:ARA_PWY-5690 TCA cycle II (plants and fungi)
Reactome:R-DME-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-DME-71403 Citric acid cycle (TCA cycle)
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-SPO-71403 Citric acid cycle (TCA cycle)
Reactome:R-DME-1430728 Metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SSC-1430728 Metabolism
Reactome:R-CEL-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-CEL-71403 Citric acid cycle (TCA cycle)
Reactome:R-HSA-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-HSA-71403 Citric acid cycle (TCA cycle)
Reactome:R-MMU-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-MMU-71403 Citric acid cycle (TCA cycle)
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-RNO-71403 Citric acid cycle (TCA cycle)
Reactome:R-SCE-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-SCE-71403 Citric acid cycle (TCA cycle)
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-DDI-71403 Citric acid cycle (TCA cycle)
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SSC-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-SSC-71403 Citric acid cycle (TCA cycle)
WikiPathways:WP2624 TCA cycle (Krebs cycle)
WikiPathways:WP4236 Krebs cycle disorders
PathBank:SMP0120613 2-Ketoglutarate Dehydrogenase Complex Deficiency
PathBank:SMP0121182 Citrate Cycle
PathBank:SMP0121340 TCA Cycle (Ubiquinol-5)
PathBank:SMP0121344 TCA Cycle (Ubiquinol-9)
PathBank:SMP0000548 Mitochondrial Complex II Deficiency
PathBank:SMP0002295 The Oncogenic Action of Fumarate
PathBank:SMP0002358 The Oncogenic Action of L-2-Hydroxyglutarate in Hydroxyglutaric aciduria
PathBank:SMP0087270 Warburg Effect
PathBank:SMP0108760 Glutaminolysis and Cancer
PathBank:SMP0120612 Mitochondrial Complex II Deficiency
PathBank:SMP0120922 LPS and Citrate Signaling and Inflammation
PathBank:SMP0121339 TCA Cycle (Ubiquinol-4)
PathBank:SMP0121343 TCA Cycle (Ubiquinol-8)
PathBank:SMP0122219 TCA Cycle (Ubiquinol-0)
PathBank:SMP0000547 Fumarase Deficiency
PathBank:SMP0000551 Pyruvate Dehydrogenase Deficiency (E2)
PathBank:SMP0002292 The Oncogenic Action of Succinate
PathBank:SMP0002377 Purine Nucleotides De Novo Biosynthesis
PathBank:SMP0108758 The Oncogenic Action of 2-Hydroxyglutarate
PathBank:SMP0120610 Congenital Lactic Acidosis
PathBank:SMP0120614 Pyruvate Dehydrogenase Deficiency (E3)
PathBank:SMP0121269 TCA Cycle
PathBank:SMP0121337 TCA Cycle (Ubiquinol-2)
PathBank:SMP0121341 TCA Cycle (Ubiquinol-6)
PathBank:SMP0121345 TCA Cycle (Ubiquinol-10)
PathBank:SMP0000057 Citric Acid Cycle
PathBank:SMP0000549 2-Ketoglutarate Dehydrogenase Complex Deficiency
PathBank:SMP0000654 Warburg Effect
PathBank:SMP0002359 The Oncogenic Action of D-2-Hydroxyglutarate in Hydroxyglutaric aciduria
PathBank:SMP0002414 TCA Cycle
PathBank:SMP0087163 Citric Acid Cycle
PathBank:SMP0087424 Citric Acid Cycle
PathBank:SMP0120611 Fumarase Deficiency
PathBank:SMP0120615 Pyruvate Dehydrogenase Deficiency (E2)
PathBank:SMP0121338 TCA Cycle (Ubiquinol-3)
PathBank:SMP0121342 TCA Cycle (Ubiquinol-7)
PathBank:SMP0000546 Congenital Lactic Acidosis
PathBank:SMP0000550 Pyruvate Dehydrogenase Deficiency (E3)
PathBank:SMP0002291 The Oncogenic Action of 2-Hydroxyglutarate
PathBank:SMP0002298 Glutaminolysis and Cancer
PathBank:SMP0002304 TCA Cycle
PathBank:SMP0086930 Warburg Effect
PathBank:SMP0087527 Warburg Effect
PathBank:SMP0063477 Citric Acid Cycle
PathBank:SMP0063681 Transfer of Acetyl Groups into Mitochondria