EC: 5.4.99.5

chorismate mutase (Chorismate pyruvatemutase)

enzyme lambda metabolic reaction experiment
uniprot:P07023 [chorismate mutase(Enzyme) mutant H239N]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [chorismate mutase(Enzyme) wildtype]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [chorismate mutase(Enzyme) mutant H257A]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [chorismate mutase(Enzyme) mutant H347N]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [chorismate mutase(Enzyme) mutant H131A]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [chorismate mutase(Enzyme) mutant H265A]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [chorismate mutase(Enzyme) mutant H197N]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [chorismate mutase(Enzyme) mutant H245N]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [chorismate mutase(Enzyme) mutant H153N]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P19080 [chorismate mutase(Enzyme) wildtype]

Chorismate -> Prephenate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.5
Temperature: 30
uniprot:P19080 [chorismate mutase(Enzyme) mutant C88K/R90S]

Chorismate -> Prephenate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.5
Temperature: 30
uniprot:P19080 [chorismate mutase(Enzyme) mutant C88S/R90K]

Chorismate -> Prephenate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.5
Temperature: 30
uniprot:P19080 [chorismate mutase(Enzyme) mutant R90G]

Chorismate -> Prephenate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.5
Temperature: 30
uniprot:P42738 [chorismate mutase(Enzyme) wildtype CM1]

Chorismate -> Prephenate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-Cl
PH: 8
Temperature: 30
uniprot:Q9S7H4 [chorismate mutase(Enzyme) wildtype CM2]

Chorismate -> Prephenate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-Cl
PH: 8
Temperature: 30
uniprot:Q9C544 [chorismate mutase(Enzyme) wildtype CM3]

Chorismate -> Prephenate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-Cl
PH: 8
Temperature: 30
- [Tryptophan]

Chorismate -> Prephenate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Imidazole-HCl
PH: 7.5
Temperature: 25
- [chorismate mutase(Enzyme) wildtype]

Chorismate -> Prephenate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M Tris-HCl
PH: 7
Temperature: 25
- [chorismate mutase(Enzyme) wildtype]

Chorismate -> Prephenate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M Tris-HCl
PH: 7.5
Temperature: 25
- [Tryptophan]

Chorismate -> Prephenate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Imidazole-HCl
PH: 8
Temperature: 25
- [Tryptophan]

Chorismate -> Prephenate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Imidazole-HCl
PH: 6
Temperature: 25
- [Tryptophan]

Chorismate -> Prephenate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Imidazole-HCl
PH: 7
Temperature: 25
- [chorismate mutase(Enzyme) wildtype]

Chorismate -> Prephenate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 0.1 M Tris-HCl
PH: 8
Temperature: 25
- [chorismate mutase(Enzyme) wildtype]

Chorismate -> Prephenate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl
PH: 8.5
Temperature: 25
uniprot:P9WIB9 [chorismate mutase(Enzyme) wildtype C-terminal His6-tagged]

Chorismate -> Prephenate		 (( (Vmax * S) ) / (Km + S)) buffer: 10 mM Tris-HCl
PH: 7.5
Temperature: 30
uniprot:P9WIC1 [chorismate mutase(Enzyme) wildtype C-terminal His6-tagged]

Chorismate -> Prephenate		 (( (Vmax * S) ) / (Km + S)) buffer: 10 mM Tris-HCl
PH: 7.5
Temperature: 30
uniprot:P9WIB9 [chorismate mutase(Enzyme) wildtype]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 0.5 mM EDTA, 0.1 mg/ml BSA, 10 mM beta-mercaptoethanol
PH: 7.5
Temperature: 37
uniprot:P9WIB9 [chorismate mutase(Enzyme) wildtype]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 0.5 mM EDTA, 0.1 mg/ml BSA, 10 mM beta-mercaptoethanol
PH: 7.5
Temperature: 37
uniprot:P9WIB9 [chorismate mutase(Enzyme) wildtype]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM sodium acetate, 33 mM 2-(N-morpholino)- ethanesulfonic acid, 33 mM 1,3-bis[tris(hydroxymethyl)- methylamino]propane, 100 mM sodium chloride
PH: 7.5
Temperature: 37
uniprot:P9WIB9 [chorismate mutase(Enzyme) wildtype]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM sodium acetate, 33 mM 2-(N-morpholino)- ethanesulfonic acid, 33 mM 1,3-bis[tris(hydroxymethyl)- methylamino]propane, 100 mM sodium chloride
PH: 6.5
Temperature: 37
uniprot:P9WIB9 [chorismate mutase(Enzyme) wildtype]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM sodium acetate, 33 mM 2-(N-morpholino)- ethanesulfonic acid, 33 mM 1,3-bis[tris(hydroxymethyl)- methylamino]propane, 100 mM sodium chloride
PH: 4
Temperature: 37
uniprot:P9WIB9 [chorismate mutase(Enzyme) wildtype]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM sodium acetate, 33 mM 2-(N-morpholino)- ethanesulfonic acid, 33 mM 1,3-bis[tris(hydroxymethyl)- methylamino]propane, 100 mM sodium chloride
PH: 9.5
Temperature: 37
uniprot:Q7CHH5 [chorismate mutase(Enzyme) wildtype *YpCM]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 0.5 mM EDTA, 0.1 mg/ml BSA, 10 mM beta-mercaptoethanol
PH: 7.5
Temperature: 37
uniprot:P9WIB9 [chorismate mutase(Enzyme) mutant E109Q]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM sodium acetate, 33 mM 2-(N-morpholino)- ethanesulfonic acid, 33 mM 1,3-bis[tris(hydroxymethyl)- methylamino]propane, 100 mM sodium chloride
PH: 4
Temperature: 37
uniprot:P9WIB9 [chorismate mutase(Enzyme) mutant E109Q]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 33 mM sodium acetate, 33 mM 2-(N-morpholino)- ethanesulfonic acid, 33 mM 1,3-bis[tris(hydroxymethyl)- methylamino]propane, 100 mM sodium chloride
PH: 7.5
Temperature: 37
uniprot:P9WIC1 [chorismate mutase(Enzyme) wildtype 105-MtCM]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 0.5 mM EDTA, 0.1 mg/ml BSA, 10 mM beta-mercaptoethanol
PH: 7.5
Temperature: 37
uniprot:P9WIC1 [chorismate mutase(Enzyme) wildtype 90-MtCM]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 0.5 mM EDTA, 0.1 mg/ml BSA, 10 mM beta-mercaptoethanol
PH: 7.5
Temperature: 37
uniprot:P9WIC1 [chorismate mutase(Enzyme) wildtype 217-MtCM]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 0.5 mM EDTA, 0.1 mg/ml BSA, 10 mM beta-mercaptoethanol
PH: 7.5
Temperature: 37
uniprot:Q57696 [chorismate mutase(Enzyme) mutant MJ0246]

Chorismate -> Prephenate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 33 mM sodium acetate, 33 mM 2-(N-morpholino)- ethanesulfonic acid, 33 mM 1,3-bis[tris(hydroxymethyl)- methylamino]propane, 100 mM sodium chloride
PH: 5
Temperature: 30
uniprot:Q57696 [chorismate mutase(Enzyme) mutant MJ0246]

Chorismate -> Prephenate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 33 mM sodium acetate, 33 mM 2-(N-morpholino)- ethanesulfonic acid, 33 mM 1,3-bis[tris(hydroxymethyl)- methylamino]propane, 100 mM sodium chloride
PH: 5
Temperature: 20
uniprot:P0A9J8 [chorismate mutase(Enzyme) mutant P*300]

Chorismate -> Prephenate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-Cl, 2.5 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 7.8
Temperature: 37
uniprot:P0A9J8 [chorismate mutase(Enzyme) mutant P*285]

Chorismate -> Prephenate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-Cl, 2.5 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 7.8
Temperature: 37
uniprot:P0A9J8 [chorismate mutase(Enzyme) wildtype P-protein]

Chorismate -> Prephenate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-Cl, 2.5 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 7.8
Temperature: 37
uniprot:P0A9J8 [chorismate mutase(Enzyme) mutant C216A]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-Cl, 2.5 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 7.8
Temperature: 37
uniprot:P0A9J8 [chorismate mutase(Enzyme) mutant C216S]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-Cl, 2.5 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 7.8
Temperature: 37
uniprot:P0A9J8 [chorismate mutase(Enzyme) mutant T278A]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-Cl, 2.5 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 7.8
Temperature: 37
uniprot:P0A9J8 [chorismate mutase(Enzyme) wildtype]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-Cl, 2.5 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 7.8
Temperature: 37
uniprot:P0A9J8 [chorismate mutase(Enzyme) mutant N160A]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-Cl, 2.5 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 7.8
Temperature: 37
uniprot:P0A9J8 [chorismate mutase(Enzyme) mutant Q215A]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-Cl, 2.5 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 7.8
Temperature: 37
uniprot:P0A9J8 [chorismate mutase(Enzyme) mutant E159A]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-Cl, 2.5 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 7.8
Temperature: 37
uniprot:P0A9J8 [chorismate mutase(Enzyme) mutant T278S]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-Cl, 2.5 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 7.8
Temperature: 37
uniprot:P0A9J8 [chorismate mutase(Enzyme) mutant T278V]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-Cl, 2.5 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 7.8
Temperature: 37
uniprot:P0A9J8 [chorismate mutase(Enzyme) mutant W226A]

Chorismate -> Prephenate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-Cl, 2.5 mM EDTA, 0.01% bovine serum albumin, 20 mM 2-mercaptoethanol
PH: 7.8
Temperature: 37
uniprot:A0A0D1DWQ2 [chorismate mutase(Enzyme) wildtype Cmu1 (aa 22-290)]

Chorismate -> Prephenate		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 100 mM NaCl
PH: 7
Temperature: 30
uniprot:A0A0D1DWQ2 [chorismate mutase(Enzyme) wildtype Cmu1 (aa 22-290)]

Chorismate -> Prephenate		 (( (Vmax * S) ) / (Km + S)) buffer: 40 mM Tris-HCl, 100 mM NaCl
PH: 7
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-6628 superpathway of L-phenylalanine biosynthesis
BioCyc:META_PHESYN L-phenylalanine biosynthesis I
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:YEAST_PHESYN L-phenylalanine biosynthesis
BioCyc:ARA_PWY-3481 superpathway of L-phenylalanine and L-tyrosine biosynthesis
BioCyc:MTBCDC1551_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:ECOO157_PWY-6630 superpathway of tyrosine biosynthesis
BioCyc:BSUB_PHESYN L-phenylalanine biosynthesis I
BioCyc:ECOL316407_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:META_PWY-6406 salicylate biosynthesis I
BioCyc:META_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:MTBH37RV_PWY-6406 salicylate biosynthesis I
BioCyc:MTBH37RV_TYRSYN L-tyrosine biosynthesis I
BioCyc:ECOO157_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:BSUB_ARO-PWY chorismate biosynthesis I
BioCyc:ECOL316407_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:META_TYRSYN L-tyrosine biosynthesis I
BioCyc:META_PWY-3481 superpathway of L-phenylalanine and L-tyrosine biosynthesis
BioCyc:META_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:YEAST_COMPLETE-ARO-YEAST-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
BioCyc:YEAST_PWY3O-4120 tyrosine biosynthesis
BioCyc:ARA_PWY-3461 L-tyrosine biosynthesis II
BioCyc:MTBH37RV_PHESYN L-phenylalanine biosynthesis I
BioCyc:MTBH37RV_PWY-5837 2-carboxy-1,4-naphthoquinol biosynthesis
BioCyc:MTBCDC1551_PWY-6627 salinosporamide A biosynthesis
BioCyc:ECOO157_PWY-6628 superpathway of phenylalanine biosynthesis
BioCyc:BSUB_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:BSUB_PWY-7626 bacilysin biosynthesis
BioCyc:BSUB_TYRSYN L-tyrosine biosynthesis I
BioCyc:META_PWY-3461 L-tyrosine biosynthesis II
BioCyc:META_PWY-3462 L-phenylalanine biosynthesis II
BioCyc:ECO_TYRSYN L-tyrosine biosynthesis I
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_PHESYN L-phenylalanine biosynthesis I
BioCyc:ARA_COMPLETE-ARO-ARA-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
BioCyc:ARA_PWY-3462 L-phenylalanine biosynthesis II
BioCyc:MTBH37RV_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:MTBCDC1551_TYRSYN L-tyrosine biosynthesis I
BioCyc:ECOO157_TYRSYN tyrosine biosynthesis I
BioCyc:ECOO157_PHESYN phenylalanine biosynthesis I
BioCyc:BSUB_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:BSUB_PWY-6164 3-dehydroquinate biosynthesis I
BioCyc:ECOL316407_TYRSYN tyrosine biosynthesis I
BioCyc:ECOL316407_PHESYN phenylalanine biosynthesis I
PathBank:SMP0000826 Tyrosine Biosynthesis
PathBank:SMP0000827 Phenylalanine Biosynthesis
PathBank:SMP0000938 Phenylalanine Metabolism
PlantCyc:ARA_COMPLETE-ARO-ARA-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
PlantCyc:ARA_PWY-3481 superpathway of L-phenylalanine and L-tyrosine biosynthesis
PlantCyc:ARA_PWY-3462 L-phenylalanine biosynthesis II
PlantCyc:ARA_PWY-3461 L-tyrosine biosynthesis II
PlantCyc:PLANT_PWY-3462 L-phenylalanine biosynthesis II
PlantCyc:PLANT_PWY-3481 superpathway of L-phenylalanine and L-tyrosine biosynthesis
PlantCyc:PLANT_PWY-3461 L-tyrosine biosynthesis II
PathBank:SMP0002442 Phenylalanine Metabolism
PathBank:SMP0002352 Tyrosine Metabolism
PathBank:SMP0002349 Phenylalanine Metabolism