EC: 5.3.1.24

phosphoribosylanthranilate isomerase (N-(5-phospho-beta-D-ribosyl)anthranilate aldose-ketose-isomerase)

enzyme lambda metabolic reaction experiment
uniprot:P16250 [phosphoribosylanthranilate isomerase(Enzyme) wildtype]

N_(5_Phospho_D_ribosyl)anthranilate -> 1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 0.5 mM MgCl2, molar excess of anthranilate phosphoribosyl transferase and indole glycerol phosphate synthase
PH: 7.5
Temperature: 25
uniprot:Q56320 [phosphoribosylanthranilate isomerase(Enzyme) mutant D126N]

N_(5_Phospho_D_ribosyl)anthranilate -> 1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2 mM EDTA, 1 mM DTT
PH: 7.5
Temperature: 25
uniprot:P00909 [phosphoribosylanthranilate isomerase(Enzyme) wildtype IGPS:PRAI]

N_(5_Phospho_D_ribosyl)anthranilate -> 1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M Tris-HCl, 4 mM K2-MgEDTA, 2 mM DTE
PH: 7.5
Temperature: 25
uniprot:P00909 [phosphoribosylanthranilate isomerase(Enzyme) mutant PRAI[ML256-452]]

N_(5_Phospho_D_ribosyl)anthranilate -> 1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M Tris-HCl, 4 mM K2-MgEDTA, 2 mM DTE
PH: 7.5
Temperature: 25
uniprot:P00909 [phosphoribosylanthranilate isomerase(Enzyme) wildtype]

N_(5_Phospho_D_ribosyl)anthranilate -> 1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2 mM DTT, 4 mM K2MgEDTA
PH: 8.6
Temperature: 25
uniprot:P00909 [phosphoribosylanthranilate isomerase(Enzyme) wildtype]

N_(5_Phospho_D_ribosyl)anthranilate -> 1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M triethanolamine/HCl, 4 mM K2MgEDTA
PH: 8.6
Temperature: 25
uniprot:P00909 [phosphoribosylanthranilate isomerase(Enzyme) wildtype]

N_(5_Phospho_D_ribosyl)anthranilate -> 1_(2_Carboxyphenylamino)_1_deoxy_D_ribulose_5_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Bistris/propane, 4 mM K2MgEDTA
PH: 8.6
Temperature: 25

Pathways

pathway id name
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:YEAST_TRPSYN-PWY tryptophan biosynthesis
BioCyc:MTBCDC1551_HISTSYN-PWY L-histidine biosynthesis
BioCyc:CAULO_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:BSUB_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:ECOL316407_TRPSYN-PWY tryptophan biosynthesis
BioCyc:ECOL316407_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:CORYNE_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:GCF_000013425_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:VCHO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:VCHO_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:HPY_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:CAULO_TRPSYN-PWY tryptophan biosynthesis
BioCyc:ANTHRA_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:AGRO_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:AGRO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:ECOL316407_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:CORYNE_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:META_PWY-7539 6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia)
BioCyc:META_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:YEAST_COMPLETE-ARO-YEAST-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
BioCyc:VCHO_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:MTBH37RV_HISTSYN-PWY L-histidine biosynthesis
BioCyc:CORYNE_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:META_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:META_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:ARA_COMPLETE-ARO-ARA-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
BioCyc:ARA_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:VCHO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:ANTHRA_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:BSUB_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:SYNEL_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:SYNEL_TRPSYN-PWY L-tryptophan biosynthesis
BioCyc:GCF_000013425_PWY-6629 superpathway of L-tryptophan biosynthesis
PathBank:SMP0000835 Tryptophan Metabolism
PathBank:SMP0000981 Trp Operon
PathBank:SMP0001930 Tryptophan Metabolism II
PlantCyc:ARA_COMPLETE-ARO-ARA-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
PlantCyc:ARA_TRPSYN-PWY L-tryptophan biosynthesis
PlantCyc:PLANT_TRPSYN-PWY L-tryptophan biosynthesis
PathBank:SMP0002353 Tryptophan Metabolism
PathBank:SMP0002445 Tryptophan Metabolism