EC: 4.1.3.4

hydroxymethylglutaryl-CoA lyase ((S)-3-hydroxy-3-methylglutaryl-CoA acetoacetate-lyase (acetyl-CoA-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q8TB92 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetoacetate + Acetyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris/Cl, 1.5 mM NAD+, 0.05 mM NADH, 5.0 mM dithiothreitol, 2.5 mM malate, 20 µg/ml malate dehydrogenase, 20 µg/ml citrate synthase
PH: 8.2
Temperature: 30
uniprot:Q8TB92 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris/Cl, 1.5 mM NAD+, 0.05 mM NADH, 5.0 mM dithiothreitol, 2.5 mM malate, 20 µg/ml malate dehydrogenase, 20 µg/ml citrate synthase
PH: 8.2
Temperature: 30
uniprot:Q8TB92 [Mn2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris/Cl, 1.5 mM NAD+, 0.05 mM NADH, 5.0 mM dithiothreitol, 2.5 mM malate, 20 µg/ml malate dehydrogenase, 20 µg/ml citrate synthase
PH: 8.2
Temperature: 30
uniprot:Q8TB92 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetoacetate + Acetyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris/Cl, 1.5 mM NAD+, 0.05 mM NADH, 5.0 mM dithiothreitol, 2.5 mM malate, 20 µg/ml malate dehydrogenase, 20 µg/ml citrate synthase
PH: 8.2
Temperature: 30
uniprot:Q8TB92 [Mn2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris/Cl, 1.5 mM NAD+, 0.05 mM NADH, 5.0 mM dithiothreitol, 2.5 mM malate, 20 µg/ml malate dehydrogenase, 20 µg/ml citrate synthase
PH: 8.2
Temperature: 30
uniprot:Q8TB92 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetoacetate + Acetyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris/Cl, 1.5 mM NAD+, 0.05 mM NADH, 5.0 mM dithiothreitol, 2.5 mM malate, 20 µg/ml malate dehydrogenase, 20 µg/ml citrate synthase
PH: 8.2
Temperature: 30
uniprot:P35914 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris/Cl, 1.5 mM of NAD+, 0.05 mM NADH, 5.0 mM dithiothreitol, 2.5 mM malate, 20 µg/ml malate dehydrogenase, 20 µg/ml citrate synthase
PH: 9
Temperature: 30
uniprot:P35914 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris/Cl, 1.5 mM of NAD+, 0.05 mM NADH, 5.0 mM dithiothreitol, 2.5 mM malate, 20 µg/ml malate dehydrogenase, 20 µg/ml citrate synthase
PH: 8
Temperature: 30
uniprot:Q8TB92 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetoacetate + Acetyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris/Cl, 1.5 mM of NAD+, 0.05 mM NADH, 5.0 mM dithiothreitol, 2.5 mM malate, 20 µg/ml malate dehydrogenase, 20 µg/ml citrate synthase
PH: 8
Temperature: 30
uniprot:Q8TB92 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris/Cl, 1.5 mM of NAD+, 0.05 mM NADH, 5.0 mM dithiothreitol, 2.5 mM malate, 20 µg/ml malate dehydrogenase, 20 µg/ml citrate synthase
PH: 9
Temperature: 30
uniprot:P35914 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris/Cl, 1.5 mM of NAD+, 0.05 mM NADH, 5.0 mM dithiothreitol, 2.5 mM malate, 20 µg/ml malate dehydrogenase, 20 µg/ml citrate synthase
PH: 9
Temperature: 30
uniprot:P35914 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris/Cl, 1.5 mM of NAD+, 0.05 mM NADH, 5.0 mM dithiothreitol, 2.5 mM malate, 20 µg/ml malate dehydrogenase, 20 µg/ml citrate synthase
PH: 8
Temperature: 30
uniprot:Q8TB92 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetoacetate + Acetyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris/Cl, 1.5 mM of NAD+, 0.05 mM NADH, 5.0 mM dithiothreitol, 2.5 mM malate, 20 µg/ml malate dehydrogenase, 20 µg/ml citrate synthase
PH: 8
Temperature: 30
uniprot:Q8TB92 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetoacetate + Acetyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris/Cl, 1.5 mM of NAD+, 0.05 mM NADH, 5.0 mM dithiothreitol, 2.5 mM malate, 20 µg/ml malate dehydrogenase, 20 µg/ml citrate synthase
PH: 9
Temperature: 30
uniprot:P35914 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris/Cl, 1.5 mM NAD+, 0.05 mM NADH, 0.5 mM dithiothreitol, 2.5 mM malate, 20 µg/ml malate dehydrogenase, 20 µg/ml citrate synthase
PH: 8.2
Temperature: 30
uniprot:P35914 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris/Cl, 1.5 mM NAD+, 0.05 mM NADH, 0.5 mM dithiothreitol, 2.5 mM malate, 20 µg/ml malate dehydrogenase, 20 µg/ml citrate synthase
PH: 8.2
Temperature: 30
- [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris/Cl, 1.5 mM NAD+, 0.05 mM NADH, 0.5 mM dithiothreitol, 2.5 mM malate, 20 µg/ml malate dehydrogenase, 20 µg/ml citrate synthase
PH: 8.2
Temperature: 30
uniprot:P35914 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 1.5 mM NAD+, 0.05 mM NADH, 5.0 mM Dithiothreitol, 2.5 mM Malate, 20 µg/ml Malate dehydrogenase, 20 µg/ml Citrate synthase
PH: 8.2
Temperature: 30
uniprot:P35914 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 1.5 mM NAD+, 0.05 mM NADH, 5.0 mM Dithiothreitol, 2.5 mM Malate, 20 µg/ml Malate dehydrogenase, 20 µg/ml Citrate synthase
PH: 8.2
Temperature: 30
uniprot:P35914 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetoacetate + Acetyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 1.5 mM NAD+, 0.05 mM NADH, 5.0 mM Dithiothreitol, 2.5 mM Malate, 20 µg/ml Malate dehydrogenase, 20 µg/ml Citrate synthase
PH: 8.2
Temperature: 30
uniprot:P35914 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetoacetate + Acetyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 1.5 mM NAD+, 0.05 mM NADH, 5.0 mM Dithiothreitol, 2.5 mM Malate, 20 µg/ml Malate dehydrogenase, 20 µg/ml Citrate synthase
PH: 8.2
Temperature: 30
uniprot:P35914 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * M) ) / (Km + M)) buffer: 100 mM Tris-HCl, 0.1 mM EDTA
PH: 8.2
Temperature: 30
uniprot:P35914 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * M) ) / (Km + M)) buffer: 100 mM Tris-HCl, 0.1 mM EDTA
PH: 8.2
Temperature: 30
uniprot:P35914 [Mn2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetoacetate + Acetyl_CoA		 (( (Vmax * M) ) / (Km + M)) buffer: 100 mM Tris-HCl, 0.1 mM EDTA
PH: 8.2
Temperature: 30
uniprot:P35914 [Mn2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * M) ) / (Km + M)) buffer: 100 mM Tris-HCl, 0.1 mM EDTA
PH: 8.2
Temperature: 30
uniprot:P35914 [Mn2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * M) ) / (Km + M)) buffer: 100 mM Tris-HCl, 0.1 mM EDTA
PH: 8.2
Temperature: 30
uniprot:P35914 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * M) ) / (Km + M)) buffer: 100 mM Tris-HCl, 0.1 mM EDTA
PH: 8.2
Temperature: 30
uniprot:P35914 [Mg2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetoacetate + Acetyl_CoA		 (( (Vmax * M) ) / (Km + M)) buffer: 100 mM Tris-HCl, 0.1 mM EDTA
PH: 8.2
Temperature: 30
uniprot:P35914 [Mn2+]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * M) ) / (Km + M)) buffer: 100 mM Tris-HCl, 0.1 mM EDTA
PH: 8.2
Temperature: 30
uniprot:P35914 [hydroxymethylglutaryl-CoA lyase(Enzyme) wildtype]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 1.5 mM NAD+, 0.05 mM NADH, 5.0 mM Dithiothreitol, 2.5 mM Malate, 20 µg/ml Malate dehydrogenase, 20 µg/ml Citrate synthase
PH: 8.2
Temperature: 30
uniprot:P35914 [hydroxymethylglutaryl-CoA lyase(Enzyme) mutant C266S/C323S]

3_Hydroxy_3_methylglutaryl_CoA -> Acetoacetate + Acetyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 1.5 mM NAD+, 0.05 mM NADH, 5.0 mM Dithiothreitol, 2.5 mM Malate, 20 µg/ml Malate dehydrogenase, 20 µg/ml Citrate synthase
PH: 8.2
Temperature: 30
uniprot:P35914 [hydroxymethylglutaryl-CoA lyase(Enzyme) mutant C266S]

3_Hydroxy_3_methylglutaryl_CoA -> Acetoacetate + Acetyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 1.5 mM NAD+, 0.05 mM NADH, 5.0 mM Dithiothreitol, 2.5 mM Malate, 20 µg/ml Malate dehydrogenase, 20 µg/ml Citrate synthase
PH: 8.2
Temperature: 30
uniprot:P35914 [hydroxymethylglutaryl-CoA lyase(Enzyme) mutant C266A]

3_Hydroxy_3_methylglutaryl_CoA -> Acetoacetate + Acetyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 1.5 mM NAD+, 0.05 mM NADH, 5.0 mM Dithiothreitol, 2.5 mM Malate, 20 µg/ml Malate dehydrogenase, 20 µg/ml Citrate synthase
PH: 8.2
Temperature: 30
uniprot:P35914 [hydroxymethylglutaryl-CoA lyase(Enzyme) mutant C323S]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 1.5 mM NAD+, 0.05 mM NADH, 5.0 mM Dithiothreitol, 2.5 mM Malate, 20 µg/ml Malate dehydrogenase, 20 µg/ml Citrate synthase
PH: 8.2
Temperature: 30
uniprot:P35914 [hydroxymethylglutaryl-CoA lyase(Enzyme) mutant C266A/C323S]

3_Hydroxy_3_methylglutaryl_CoA -> Acetyl_CoA + Acetoacetate		 (( (Vmax * S) ) / (Km + S)) buffer: 200 mM Tris-HCl, 10 mM MgCl2, 1.5 mM NAD+, 0.05 mM NADH, 5.0 mM Dithiothreitol, 2.5 mM Malate, 20 µg/ml Malate dehydrogenase, 20 µg/ml Citrate synthase
PH: 8.2
Temperature: 30

Pathways

pathway id name
BioCyc:HUMAN_PWY66-367 ketogenesis
BioCyc:META_PWY-6672 cis-genanyl-CoA degradation
BioCyc:MOUSE_PWY66-367 ketogenesis
BioCyc:HUMAN_LEU-DEG2-PWY leucine degradation
BioCyc:META_PWY-5074 mevalonate degradation
BioCyc:ARA_LEU-DEG2-PWY L-leucine degradation I
BioCyc:MOUSE_PWY3DJ-6 Leucine Catabolism
BioCyc:MOUSE_LEU-DEG2-PWY leucine degradation I
BioCyc:META_LEU-DEG2-PWY L-leucine degradation I
BioCyc:META_PWY66-367 ketogenesis
BioCyc:MOUSE_PWY3FA-941 ketogenesis
PlantCyc:ARA_LEU-DEG2-PWY L-leucine degradation I
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-77111 Synthesis of Ketone Bodies
Reactome:R-RNO-74182 Ketone body metabolism
Reactome:R-BTA-77111 Synthesis of Ketone Bodies
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-77111 Synthesis of Ketone Bodies
Reactome:R-MMU-74182 Ketone body metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-77111 Synthesis of Ketone Bodies
Reactome:R-BTA-74182 Ketone body metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-74182 Ketone body metabolism
WikiPathways:WP4686 Leucine, isoleucine and valine metabolism
WikiPathways:WP4190 Mevalonate arm of cholesterol biosynthesis pathway
PathBank:SMP0120662 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0120806 3-Hydroxyisobutyric Aciduria
PathBank:SMP0120440 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0120444 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0120516 Maple Syrup Urine Disease
PathBank:SMP0120589 Isovaleric Acidemia
PathBank:SMP0120633 Succinyl CoA: 3-Ketoacid CoA Transferase Deficiency
PathBank:SMP0000032 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0000173 beta-Ketothiolase Deficiency
PathBank:SMP0000199 Maple Syrup Urine Disease
PathBank:SMP0000384 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000141 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0000524 Isovaleric Acidemia
PathBank:SMP0002433 Leucine Degradation
PathBank:SMP0120661 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0120665 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0120745 Methylmalonic Aciduria
PathBank:SMP0120757 Propionic Acidemia
PathBank:SMP0120805 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0120439 2-Methyl-3-hydroxybutryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120443 3-Methylglutaconic Aciduria Type III
PathBank:SMP0120459 beta-Ketothiolase Deficiency
PathBank:SMP0120503 Isovaleric Aciduria
PathBank:SMP0120523 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120588 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000071 Ketone Body Metabolism
PathBank:SMP0000073 Butyrate Metabolism
PathBank:SMP0000138 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0000140 3-Methylglutaconic Aciduria Type III
PathBank:SMP0000238 Isovaleric Aciduria
PathBank:SMP0000523 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0063603 Butyrate Metabolism
PathBank:SMP0063689 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0120660 2-Methyl-3-hydroxybutryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120664 3-Methylglutaconic Aciduria Type III
PathBank:SMP0120736 Maple Syrup Urine Disease
PathBank:SMP0120808 Isovaleric Acidemia
PathBank:SMP0120852 Succinyl CoA: 3-Ketoacid CoA Transferase Deficiency
PathBank:SMP0120441 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0120525 Methylmalonic Aciduria
PathBank:SMP0120537 Propionic Acidemia
PathBank:SMP0120586 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0000137 2-Methyl-3-hydroxybutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000237 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0000200 Methylmalonic Aciduria
PathBank:SMP0000521 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0000569 Succinyl CoA: 3-Ketoacid CoA Transferase Deficiency
PathBank:SMP0120663 3-Methylglutaconic Aciduria Type I
PathBank:SMP0120679 beta-Ketothiolase Deficiency
PathBank:SMP0120723 Isovaleric Aciduria
PathBank:SMP0120743 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120807 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120442 3-Methylglutaconic Aciduria Type I
PathBank:SMP0120587 3-Hydroxyisobutyric Aciduria
PathBank:SMP0000236 Propionic Acidemia
PathBank:SMP0000139 3-Methylglutaconic Aciduria Type I
PathBank:SMP0000522 3-Hydroxyisobutyric Aciduria
PathBank:SMP0087287 Butyrate Metabolism
PathBank:SMP0087299 Ketone Body Metabolism
PathBank:SMP0087327 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0012467 Butanoate Metabolism
PathBank:SMP0063631 Ketone Body Metabolism