EC: 4.1.1.33

diphosphomevalonate decarboxylase (ATP:(R)-5-diphosphomevalonate carboxy-lyase (adding ATP; isopentenyl-diphosphate-forming))

enzyme lambda metabolic reaction experiment
uniprot:P53602 [diphosphomevalonate decarboxylase(Enzyme) wildtype]

ATP + (R)_5_Diphosphomevalonate -> Phosphate + Isopentenyl_diphosphate + ADP + CO2		 (( (V * B) ) / (Km + B)) buffer: 50 mM MOPS-KOH, 6 mM MgCl2, 10 mM KF, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml pyruvate kinase, 10 U/ml lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P53602 [diphosphomevalonate decarboxylase(Enzyme) wildtype]

(R)_5_Diphosphomevalonate + ATP -> CO2 + Phosphate + Isopentenyl_diphosphate + ADP		 (( (V * A) ) / (Km + A)) buffer: 50 mM MOPS-KOH, 6 mM MgCl2, 10 mM KF, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml pyruvate kinase, 10 U/ml lactate dehydrogenase
PH: 7
Temperature: 25
- [diphosphomevalonate decarboxylase(Enzyme) wildtype]

(R)_5_Diphosphomevalonate + ATP -> Isopentenyl_diphosphate + ADP + Phosphate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Phosphate, 20 mM MgCl2, 10 mM DTT
PH: 7
Temperature: 37
- [diphosphomevalonate decarboxylase(Enzyme) wildtype]

(R)_5_Diphosphomevalonate + ATP -> Phosphate + CO2 + Isopentenyl_diphosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Phosphate, 20 mM MgCl2, 10 mM DTT
PH: 7
Temperature: 37
uniprot:Q62967 [Mg2+]

ATP + (R)_5_Diphosphomevalonate -> ADP + CO2 + Phosphate + Isopentenyl_diphosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 M Tris-HCl, 10 mM Iodoacetamide, 10 mM NaF, 30 mM Nicotinamide
PH: 7
Temperature: 36
uniprot:Q62967 [Mg2+]

ATP + (R)_5_Diphosphomevalonate -> CO2 + Phosphate + Isopentenyl_diphosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 10 mM Iodoacetamide, 10 mM NaF, 30 mM Nicotinamide
PH: 7
Temperature: 36
uniprot:Q62967 [Mg2+]

(R)_5_Diphosphomevalonate + ATP -> Phosphate + CO2 + Isopentenyl_diphosphate + ADP		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 M Tris-HCl, 10 mM Iodoacetamide, 10 mM NaF, 30 mM Nicotinamide
PH: 7
Temperature: 36
uniprot:Q62967 [Mg2+]

(R)_5_Diphosphomevalonate + ATP -> Phosphate + ADP + CO2 + Isopentenyl_diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris-HCl, 10 mM Iodoacetamide, 10 mM NaF, 30 mM Nicotinamide
PH: 7
Temperature: 36
uniprot:Q62967 [Mg2+]

(R)_5_Diphosphomevalonate + ATP -> Phosphate + CO2 + ADP + Isopentenyl_diphosphate		 (( (Vmax * M) ) / (Km + M)) buffer: 0.1 M Tris-HCl, 10 mM Iodoacetamide, 10 mM NaF, 30 mM Nicotinamide
PH: 7
Temperature: 36
uniprot:Q62967 [Mg2+]

ATP + (R)_5_Diphosphomevalonate -> Phosphate + Isopentenyl_diphosphate + ADP + CO2		 (( (Vmax * M) ) / (Km + M)) buffer: 0.1 M Tris-HCl, 10 mM Iodoacetamide, 10 mM NaF, 30 mM Nicotinamide
PH: 7
Temperature: 36

Pathways

pathway id name
BioCyc:META_PWY-922 mevalonate pathway I
BioCyc:MOUSE_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:HUMAN_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:HUMAN_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
BioCyc:YEAST_ERGOSTEROL-SYN-PWY superpathway of ergosterol biosynthesis
BioCyc:YEAST_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
BioCyc:YEAST_PWY-922 mevalonate pathway
BioCyc:ARA_PWY-922 mevalonate pathway I
BioCyc:META_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
BioCyc:META_PWY-7391 isoprene biosynthesis II (engineered)
BioCyc:HUMAN_PWY-922 mevalonate pathway
BioCyc:META_ERGOSTEROL-SYN-PWY superpathway of ergosterol biosynthesis I
BioCyc:META_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:MOUSE_PWY-922 mevalonate pathway I
PlantCyc:ARA_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
PlantCyc:PLANT_PWY-922 mevalonate pathway I
PlantCyc:PLANT_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
PlantCyc:ARA_PWY-922 mevalonate pathway I
Reactome:R-HSA-9969896 Lanosterol biosynthesis
Reactome:R-MMU-9969896 Lanosterol biosynthesis
Reactome:R-SPO-9969896 Lanosterol biosynthesis
Reactome:R-BTA-9969896 Lanosterol biosynthesis
Reactome:R-DRE-9969896 Lanosterol biosynthesis
Reactome:R-DDI-9969896 Lanosterol biosynthesis
Reactome:R-RNO-9969896 Lanosterol biosynthesis
Reactome:R-SCE-9969896 Lanosterol biosynthesis
Reactome:R-BTA-597592 Post-translational protein modification
Reactome:R-BTA-446199 Synthesis of dolichyl-phosphate
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-BTA-191273 Cholesterol biosynthesis
Reactome:R-DRE-392499 Metabolism of proteins
Reactome:R-DRE-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-DDI-597592 Post-translational protein modification
Reactome:R-DDI-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-DDI-191273 Cholesterol biosynthesis
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-HSA-446199 Synthesis of dolichyl-phosphate
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-MMU-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-RNO-597592 Post-translational protein modification
Reactome:R-RNO-446199 Synthesis of dolichyl-phosphate
Reactome:R-SCE-8957322 Metabolism of steroids
Reactome:R-SCE-597592 Post-translational protein modification
Reactome:R-SCE-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-SPO-446203 Asparagine N-linked glycosylation
Reactome:R-SPO-1430728 Metabolism
Reactome:R-BTA-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-BTA-8957322 Metabolism of steroids
Reactome:R-DRE-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-DDI-392499 Metabolism of proteins
Reactome:R-DDI-446203 Asparagine N-linked glycosylation
Reactome:R-DDI-8957322 Metabolism of steroids
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-191273 Cholesterol biosynthesis
Reactome:R-HSA-2426168 Activation of gene expression by SREBF (SREBP)
Reactome:R-HSA-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-MMU-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-RNO-392499 Metabolism of proteins
Reactome:R-RNO-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-SPO-446199 Synthesis of dolichyl-phosphate
Reactome:R-BTA-392499 Metabolism of proteins
Reactome:R-BTA-446203 Asparagine N-linked glycosylation
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-556833 Metabolism of lipids
Reactome:R-DRE-8957322 Metabolism of steroids
Reactome:R-DRE-446199 Synthesis of dolichyl-phosphate
Reactome:R-DDI-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-HSA-446203 Asparagine N-linked glycosylation
Reactome:R-MMU-191273 Cholesterol biosynthesis
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-MMU-446199 Synthesis of dolichyl-phosphate
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-8957322 Metabolism of steroids
Reactome:R-RNO-446203 Asparagine N-linked glycosylation
Reactome:R-SCE-556833 Metabolism of lipids
Reactome:R-SCE-191273 Cholesterol biosynthesis
Reactome:R-SCE-446199 Synthesis of dolichyl-phosphate
Reactome:R-SPO-392499 Metabolism of proteins
Reactome:R-SPO-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-SPO-8957322 Metabolism of steroids
Reactome:R-BTA-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-DRE-191273 Cholesterol biosynthesis
Reactome:R-DRE-597592 Post-translational protein modification
Reactome:R-DRE-446203 Asparagine N-linked glycosylation
Reactome:R-DDI-446199 Synthesis of dolichyl-phosphate
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-556833 Metabolism of lipids
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-1655829 Regulation of cholesterol biosynthesis by SREBP (SREBF)
Reactome:R-HSA-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-MMU-446203 Asparagine N-linked glycosylation
Reactome:R-RNO-191273 Cholesterol biosynthesis
Reactome:R-RNO-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-SCE-392499 Metabolism of proteins
Reactome:R-SCE-446203 Asparagine N-linked glycosylation
Reactome:R-SPO-597592 Post-translational protein modification
Reactome:R-SPO-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-SPO-556833 Metabolism of lipids
Reactome:R-SPO-191273 Cholesterol biosynthesis
WikiPathways:WP4189 Mevalonate arm of cholesterol biosynthesis pathway with inhibitors
WikiPathways:WP4190 Mevalonate arm of cholesterol biosynthesis pathway
PathBank:SMP0120794 Mevalonic Aciduria
PathBank:SMP0000023 Steroid Biosynthesis
PathBank:SMP0000387 CHILD Syndrome
PathBank:SMP0000112 Risedronate Action Pathway
PathBank:SMP0000508 Cholesteryl Ester Storage Disease
PathBank:SMP0002438 Cholesterol Biosynthesis and Metabolism CE(12:0)
PathBank:SMP0087326 Steroid Biosynthesis
PathBank:SMP0120685 Chondrodysplasia Punctata II, X-Linked Dominant (CDPX2)
PathBank:SMP0120769 Hypercholesterolemia
PathBank:SMP0120793 Hyper-IgD Syndrome
PathBank:SMP0121055 Mevalonate Pathway
PathBank:SMP0000389 Smith-Lemli-Opitz Syndrome (SLOS)
PathBank:SMP0000319 Lysosomal Acid Lipase Deficiency (Wolman Disease)
PathBank:SMP0000082 Simvastatin Action Pathway
PathBank:SMP0000111 Cerivastatin Action Pathway
PathBank:SMP0000131 Atorvastatin Action Pathway
PathBank:SMP0000511 Wolman Disease
PathBank:SMP0002381 Steroid Biosynthesis
PathBank:SMP0002436 Cholesterol biosynthesis and metabolism CE(10:0)
PathBank:SMP0002441 Cholesterol biosynthesis and metabolism CE(18:0)
PathBank:SMP0087233 Steroid Biosynthesis
PathBank:SMP0120684 CHILD Syndrome
PathBank:SMP0120692 Desmosterolosis
PathBank:SMP0120732 Lysosomal Acid Lipase Deficiency (Wolman Disease)
PathBank:SMP0120792 Cholesteryl Ester Storage Disease
PathBank:SMP0070041 Cholesterol Biosynthesis and Metabolism
PathBank:SMP0000386 Desmosterolosis
PathBank:SMP0000089 Pravastatin Action Pathway
PathBank:SMP0000095 Alendronate Action Pathway
PathBank:SMP0000099 Lovastatin Action Pathway
PathBank:SMP0000117 Pamidronate Action Pathway
PathBank:SMP0000509 Hyper-IgD Syndrome
PathBank:SMP0120735 Smith-Lemli-Opitz Syndrome (SLOS)
PathBank:SMP0120795 Wolman Disease
PathBank:SMP0121209 Mevalonate Pathway
PathBank:SMP0000388 Chondrodysplasia Punctata II, X-Linked Dominant (CDPX2)
PathBank:SMP0000079 Ibandronate Action Pathway
PathBank:SMP0000092 Rosuvastatin Action Pathway
PathBank:SMP0000209 Hypercholesterolemia
PathBank:SMP0000107 Zoledronate Action Pathway
PathBank:SMP0000119 Fluvastatin Action Pathway
PathBank:SMP0000510 Mevalonic Aciduria
PathBank:SMP0002384 Terpenoid Backbone Biosynthesis
PathBank:SMP0002435 Cholesterol biosynthesis and metabolism CE(14:0)
PathBank:SMP0002440 Cholesterol Biosynthesis and Metabolism CE(16:0)