EC: 3.5.1.23

ceramidase (N-acylsphingosine amidohydrolase)

enzyme lambda metabolic reaction experiment
- [ceramidase(Enzyme) wildtype]

N_Dodecanoylsphingosine + H2O -> Sphingosine + Dodecanoate		 (( (Vmax * S) ) / (Km + S)) buffer: 250 mM Sodium acetate, 0.5 % (w/v) Triton X-100, 0.2 % (w/v) Tween 20, 0.2 % (w/v) Nonidet P-40, 0.8 % (w/v) Sodium cholate
PH: 4.2
Temperature: 37
uniprot:Q5QJU3 [Ca2+]

H2O + N_Oleoyl_D_sphingosine -> Sphingosine + Oleate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 0.15 % (v/v) Triton X-100
PH: 9
Temperature: 37
uniprot:Q5QJU3 [Ca2+]

N_Lignoceroylsphingosine + H2O -> Lignoceric_acid + Sphingosine		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 0.15 % (v/v) Triton X-100
PH: 9
Temperature: 37
uniprot:Q5QJU3 [Ca2+]

H2O + N_Eicosanoyl_D_sphingosine -> Sphingosine + Arachidic_acid		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 0.15 % (v/v) Triton X-100
PH: 9
Temperature: 37
uniprot:Q5QJU3 [Ca2+]

H2O + N_Stearoyl_D_sphingosine -> Stearate + Sphingosine		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 0.15 % (v/v) Triton X-100
PH: 9
Temperature: 37
uniprot:Q5QJU3 [Ca2+]

H2O + Nervonoylsphingosine -> Sphingosine + Nervonic_acid		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 0.15 % (v/v) Triton X-100
PH: 9
Temperature: 37
uniprot:Q5QJU3 [Ca2+]

H2O + Gondoylsphingosine -> Gondoic_acid + Sphingosine		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 0.15 % (v/v) Triton X-100
PH: 9
Temperature: 37
uniprot:Q13510 [ceramidase(Enzyme) wildtype]

N_Dodecanoylsphingosine + H2O -> Sphingosine + Dodecanoate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M citrate/phosphate, 150 mM NaCl, 0.05% BSA, 0.1% Igepal CA-630
PH: 4.5
Temperature: 37
uniprot:Q13510 [ceramidase(Enzyme) wildtype]

N_Dodecanoylsphingosine + H2O -> Sphingosine + Dodecanoate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M citrate/phosphate, 150 mM NaCl, 0.05% BSA, 0.1% Igepal CA-630
PH: 4.5
Temperature: 37
uniprot:Q5QJU3 [Ca2+]

N_Hexanoyl_D_erythro_sphingosine + H2O -> Sphingosine + Hexanoate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 0.15 % (v/v) Triton X-100
PH: 9
Temperature: 37
uniprot:Q5QJU3 [Ca2+]

N_Palmitoylsphingosine + H2O -> Sphingosine + Palmitate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 0.15 % (v/v) Triton X-100
PH: 9
Temperature: 37
uniprot:Q5QJU3 [Ca2+]

H2O + N_Dodecanoylsphingosine -> Sphingosine + Dodecanoate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 0.15 % (v/v) Triton X-100
PH: 9
Temperature: 37
uniprot:Q5QJU3 [Ca2+]

H2O + N_Myristoyl_D_sphingosine -> Myristic_acid + Sphingosine		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 0.15 % (v/v) Triton X-100
PH: 9
Temperature: 37
uniprot:Q91XT9 [ceramidase(Enzyme) wildtype]

H2O + N_Palmitoylsphingosine -> Sphingosine + Palmitate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.5% Triton X-100
PH: 7
Temperature: 37
uniprot:Q91XT9 [ceramidase(Enzyme) wildtype]

N_Palmitoylsphinganine + H2O -> Sphinganine + Palmitate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.5% Triton X-100
PH: 7
Temperature: 37
uniprot:Q13510 [ceramidase(Enzyme) wildtype]

Sphingosine + Dodecanoate -> N_Dodecanoylsphingosine + H2O		 (( (Vmax * B) ) / (Km + B)) buffer: 0.1 M citrate-phosphate, 0.05 % Triton X-100, 150 mM NaCl
PH: 6
Temperature: 37
uniprot:Q13510 [ceramidase(Enzyme) wildtype]

Sphingosine + Dodecanoate -> N_Dodecanoylsphingosine + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M citrate-phosphate, 0.05 % Triton X-100, 150 mM NaCl
PH: 6
Temperature: 37
uniprot:Q9NR71 [ceramidase(Enzyme) wildtype]

D_Erythro_dodecanoyl_7_nitrobenz_2_oxa_1,3_diazolylceramide + H2O -> Sphingosine + N_[12_(7_Nitrobenzo_2_oxa_1,3_diazolyl)amino]dodecanoic_acid		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris, 0.3% Triton-X-100
PH: 7.5
Temperature: 37
uniprot:Q9JHE3 [ceramidase(Enzyme) wildtype neutral isoenzyme]

N_Acylsphingosine + H2O -> Sphingosine + Fatty_acid		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 0.1 % Triton X-100
PH: 7.5
Temperature: 37
uniprot:Q9JHE3 [ceramidase(Enzyme) wildtype neutral isoenzyme]

N_Acylsphingosine + H2O -> Fatty_acid + Sphingosine		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 1 % sodium cholate
PH: 7.5
Temperature: 37
uniprot:Q9JHE3 [ceramidase(Enzyme) wildtype neutral isoenzyme]

N_Acylsphingosine + H2O -> Fatty_acid + Sphingosine		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 0.1 % Triton X-100
PH: 7.5
Temperature: 37
uniprot:Q9JHE3 [ceramidase(Enzyme) wildtype neutral isoenzyme]

N_Acylsphingosine + H2O -> Sphingosine + Fatty_acid		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 1 % sodium cholate
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY3DJ-11470 sphingosine and sphingosine-1-phosphate metabolism
BioCyc:HUMAN_PWY66-388 fatty acid α-oxidation III
BioCyc:META_PWY3DJ-11470 sphingosine and sphingosine-1-phosphate metabolism
BioCyc:ARA_PWY-6483 ceramide degradation
BioCyc:MOUSE_PWY3DJ-11470 sphingosine and sphingosine-1-phosphate metabolism
BioCyc:META_PWY-6483 ceramide degradation
PlantCyc:PLANT_PWY-6483 ceramide degradation
PlantCyc:ORYZA_PWY-6483 ceramide degradation
Reactome:R-BTA-9840310 Glycosphingolipid catabolism
Reactome:R-HSA-9840310 Glycosphingolipid catabolism
Reactome:R-MMU-9840310 Glycosphingolipid catabolism
Reactome:R-DME-9845614 Sphingolipid catabolism
Reactome:R-HSA-9845614 Sphingolipid catabolism
Reactome:R-HSA-9856651 MITF-M-dependent gene expression
Reactome:R-RNO-9840310 Glycosphingolipid catabolism
Reactome:R-CEL-9845614 Sphingolipid catabolism
Reactome:R-DRE-9845614 Sphingolipid catabolism
Reactome:R-HSA-9857377 Regulation of MITF-M-dependent genes involved in lysosome biogenesis and autophagy
Reactome:R-CEL-9840310 Glycosphingolipid catabolism
Reactome:R-MMU-9845614 Sphingolipid catabolism
Reactome:R-HSA-9730414 MITF-M-regulated melanocyte development
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-BTA-428157 Sphingolipid metabolism
Reactome:R-CEL-428157 Sphingolipid metabolism
Reactome:R-HSA-112315 Transmission across Chemical Synapses
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-RNO-1660662 Glycosphingolipid metabolism
Reactome:R-RNO-112316 Neuronal System
Reactome:R-CEL-556833 Metabolism of lipids
Reactome:R-DME-1430728 Metabolism
Reactome:R-HSA-1266738 Developmental Biology
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-428157 Sphingolipid metabolism
Reactome:R-MMU-1660662 Glycosphingolipid metabolism
Reactome:R-MMU-112310 Neurotransmitter release cycle
Reactome:R-RNO-112315 Transmission across Chemical Synapses
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-556833 Metabolism of lipids
Reactome:R-HSA-1660662 Glycosphingolipid metabolism
Reactome:R-HSA-112316 Neuronal System
Reactome:R-MMU-428157 Sphingolipid metabolism
Reactome:R-MMU-112315 Transmission across Chemical Synapses
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-112310 Neurotransmitter release cycle
Reactome:R-BTA-1660662 Glycosphingolipid metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-1660662 Glycosphingolipid metabolism
Reactome:R-DRE-428157 Sphingolipid metabolism
Reactome:R-DME-556833 Metabolism of lipids
Reactome:R-DME-428157 Sphingolipid metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-112310 Neurotransmitter release cycle
Reactome:R-MMU-112316 Neuronal System
Reactome:R-RNO-428157 Sphingolipid metabolism
WikiPathways:WP4690 Sphingolipid metabolism (integrated pathway)
WikiPathways:WP5192 Modulation of PI3K-Akt-mTOR signaling by bioactive sphingolipids
WikiPathways:WP4344 Sphingolipid metabolism overview
PathBank:SMP0000349 Gaucher Disease
PathBank:SMP0063667 Sphingolipid Metabolism
PathBank:SMP0120481 Gaucher Disease
PathBank:SMP0120590 Fabry Disease
PathBank:SMP0000348 Globoid Cell Leukodystrophy
PathBank:SMP0000525 Fabry Disease
PathBank:SMP0120482 Globoid Cell Leukodystrophy
PathBank:SMP0120518 Metachromatic Leukodystrophy (MLD)
PathBank:SMP0120591 Krabbe Disease
PathBank:SMP0000034 Sphingolipid Metabolism
PathBank:SMP0000347 Metachromatic Leukodystrophy (MLD)
PathBank:SMP0000526 Krabbe Disease
PathBank:SMP0087463 Sphingolipid Metabolism