EC: 3.1.2.1

acetyl-CoA hydrolase (acetyl-CoA hydrolase)

enzyme lambda metabolic reaction experiment
- [acetyl-CoA hydrolase(Enzyme) wildtype]

H2O + Acetyl_CoA -> Coenzyme_A + Acetate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 1 mM MgCl2
PH: 7.4
Temperature: 30
- [acetyl-CoA hydrolase(Enzyme) wildtype]

H2O + Acetyl_CoA -> Coenzyme_A + Acetate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 1 mM MgCl2
PH: 7.4
Temperature: 30
uniprot:Q99NB7 [acetyl-CoA hydrolase(Enzyme) wildtype isoenzyme Acot12]

Acetyl_CoA + H2O -> Coenzyme_A + Acetate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 2 mM ATP, 0.2 mM 5,5'-dithiobis(2-nitrobenzoic acid)
PH: 7.8
Temperature: 25
uniprot:Q99NB7 [acetyl-CoA hydrolase(Enzyme) wildtype isoenzyme Acot12]

H2O + Acetyl_CoA -> Acetate + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 2 mM ATP, 0.2 mM 5,5'-dithiobis(2-nitrobenzoic acid)
PH: 7.8
Temperature: 25
uniprot:A0A1E3P8S6 [acetyl-CoA hydrolase(Enzyme) wildtype C-terminal His-tagged]

Acetyl_CoA + H2O -> Coenzyme_A + Acetate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 150 mM NaCl, 0.25 mM DTNB
PH: 7.5
Temperature: 30

Pathways

pathway id name
BioCyc:HUMAN_PWY-5148 acyl-CoA hydrolysis
BioCyc:META_FAO-PWY fatty acid β-oxidation I
BioCyc:HUMAN_FAO-PWY fatty acid β-oxidation
BioCyc:MOUSE_FAO-PWY fatty acid β-oxidation I
BioCyc:MOUSE_PWY-5148 acyl-CoA hydrolysis
BioCyc:MOUSE_PWY-5136 fatty acid β-oxidation II (core pathway)
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-HSA-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-HSA-389887 Beta-oxidation of pristanoyl-CoA
Reactome:R-HSA-194068 Bile acid and bile salt metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-MMU-389887 Beta-oxidation of pristanoyl-CoA
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-RNO-8978868 Fatty acid metabolism
Reactome:R-RNO-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-RNO-77289 Mitochondrial Fatty Acid Beta-Oxidation
Reactome:R-RNO-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-BTA-8978868 Fatty acid metabolism
Reactome:R-BTA-77289 Mitochondrial Fatty Acid Beta-Oxidation
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-8978868 Fatty acid metabolism
Reactome:R-HSA-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-HSA-77289 Mitochondrial Fatty Acid Beta-Oxidation
Reactome:R-MMU-8978868 Fatty acid metabolism
Reactome:R-MMU-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-MMU-77289 Mitochondrial Fatty Acid Beta-Oxidation
Reactome:R-MMU-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-RNO-390918 Peroxisomal lipid metabolism
Reactome:R-RNO-192105 Synthesis of bile acids and bile salts
Reactome:R-HSA-192105 Synthesis of bile acids and bile salts
Reactome:R-MMU-194068 Bile acid and bile salt metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-RNO-389887 Beta-oxidation of pristanoyl-CoA
Reactome:R-RNO-8957322 Metabolism of steroids
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-390918 Peroxisomal lipid metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-MMU-390918 Peroxisomal lipid metabolism
Reactome:R-MMU-192105 Synthesis of bile acids and bile salts
Reactome:R-RNO-194068 Bile acid and bile salt metabolism
WikiPathways:WP5064 7-oxo-C and 7-beta-HC pathways
WikiPathways:WP4545 Oxysterols derived from cholesterol
WikiPathways:WP2318 Fatty acid oxidation
PathBank:SMP0120662 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0120726 Leigh Syndrome
PathBank:SMP0120806 3-Hydroxyisobutyric Aciduria
PathBank:SMP0120822 Carnitine Palmitoyl Transferase Deficiency I
PathBank:SMP0120826 Medium Chain Acyl-CoA Dehydrogenase Deficiency (MCAD)
PathBank:SMP0120842 Pyruvate Kinase Deficiency
PathBank:SMP0120440 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0120444 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0120516 Maple Syrup Urine Disease
PathBank:SMP0120589 Isovaleric Acidemia
PathBank:SMP0000032 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0000051 Fatty Acid Metabolism
PathBank:SMP0000173 beta-Ketothiolase Deficiency
PathBank:SMP0000199 Maple Syrup Urine Disease
PathBank:SMP0000384 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000235 Short-Chain Acyl-CoA Dehydrogenase Deficiency (SCAD Deficiency)
PathBank:SMP0000334 Pyruvate Decarboxylase E1 Component Deficiency (PDHE1 Deficiency)
PathBank:SMP0000482 Mitochondrial Beta-Oxidation of Long Chain Saturated Fatty Acids
PathBank:SMP0000141 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0000524 Isovaleric Acidemia
PathBank:SMP0000540 Very-Long-Chain Acyl-CoA Dehydrogenase Deficiency (VLCAD)
PathBank:SMP0000544 Long-Chain-3-Hydroxyacyl-CoA Dehydrogenase Deficiency (LCHAD)
PathBank:SMP0000568 Short-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency (SCHAD)
PathBank:SMP0087294 Fatty Acid Elongation in Mitochondria
PathBank:SMP0087358 Mitochondrial Beta-Oxidation of Short Chain Saturated Fatty Acids
PathBank:SMP0120661 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0120665 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0120745 Methylmalonic Aciduria
PathBank:SMP0120753 Pyruvate Decarboxylase E1 Component Deficiency (PDHE1 Deficiency)
PathBank:SMP0120757 Propionic Acidemia
PathBank:SMP0120805 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0120825 Carnitine Palmitoyl Transferase Deficiency II
PathBank:SMP0120841 Primary Hyperoxaluria II, PH2
PathBank:SMP0120439 2-Methyl-3-hydroxybutryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120443 3-Methylglutaconic Aciduria Type III
PathBank:SMP0120459 beta-Ketothiolase Deficiency
PathBank:SMP0120503 Isovaleric Aciduria
PathBank:SMP0120523 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120588 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120632 Short-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency (SCHAD)
PathBank:SMP0000054 Fatty Acid Elongation in Mitochondria
PathBank:SMP0000138 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0000140 3-Methylglutaconic Aciduria Type III
PathBank:SMP0000238 Isovaleric Aciduria
PathBank:SMP0000185 Glutaric Aciduria Type I
PathBank:SMP0000196 Leigh Syndrome
PathBank:SMP0000481 Mitochondrial Beta-Oxidation of Medium Chain Saturated Fatty Acids
PathBank:SMP0000523 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000539 Long Chain Acyl-CoA Dehydrogenase Deficiency (LCAD)
PathBank:SMP0000559 Pyruvate Kinase Deficiency
PathBank:SMP0087325 Pyruvate Metabolism
PathBank:SMP0063641 Mitochondrial Beta-Oxidation of Short Chain Saturated Fatty Acids
PathBank:SMP0063689 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0120660 2-Methyl-3-hydroxybutryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120664 3-Methylglutaconic Aciduria Type III
PathBank:SMP0120696 Ethylmalonic Encephalopathy
PathBank:SMP0120736 Maple Syrup Urine Disease
PathBank:SMP0120740 Short-Chain Acyl-CoA Dehydrogenase Deficiency (SCAD Deficiency)
PathBank:SMP0120808 Isovaleric Acidemia
PathBank:SMP0120824 Very-Long-Chain Acyl-CoA Dehydrogenase Deficiency (VLCAD)
PathBank:SMP0120828 Trifunctional Protein Deficiency
PathBank:SMP0120441 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0120525 Methylmalonic Aciduria
PathBank:SMP0120537 Propionic Acidemia
PathBank:SMP0120586 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0000137 2-Methyl-3-hydroxybutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000237 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0000200 Methylmalonic Aciduria
PathBank:SMP0000212 Pyruvate Dehydrogenase Complex Deficiency
PathBank:SMP0000521 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0000541 Carnitine Palmitoyl Transferase Deficiency II
PathBank:SMP0000545 Trifunctional Protein Deficiency
PathBank:SMP0087280 Fatty Acid Metabolism
PathBank:SMP0087360 Mitochondrial Beta-Oxidation of Long Chain Saturated Fatty Acids
PathBank:SMP0063640 Mitochondrial Beta-Oxidation of Medium Chain Saturated Fatty Acids
PathBank:SMP0120663 3-Methylglutaconic Aciduria Type I
PathBank:SMP0120679 beta-Ketothiolase Deficiency
PathBank:SMP0120723 Isovaleric Aciduria
PathBank:SMP0120743 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120751 Pyruvate Dehydrogenase Complex Deficiency
PathBank:SMP0120807 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120823 Long Chain Acyl-CoA Dehydrogenase Deficiency (LCAD)
PathBank:SMP0120827 Long-Chain-3-Hydroxyacyl-CoA Dehydrogenase Deficiency (LCHAD)
PathBank:SMP0120851 Short-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency (SCHAD)
PathBank:SMP0120442 3-Methylglutaconic Aciduria Type I
PathBank:SMP0120587 3-Hydroxyisobutyric Aciduria
PathBank:SMP0000060 Pyruvate Metabolism
PathBank:SMP0000236 Propionic Acidemia
PathBank:SMP0000139 3-Methylglutaconic Aciduria Type I
PathBank:SMP0000181 Ethylmalonic Encephalopathy
PathBank:SMP0000480 Mitochondrial Beta-Oxidation of Short Chain Saturated Fatty Acids
PathBank:SMP0000522 3-Hydroxyisobutyric Aciduria
PathBank:SMP0000538 Carnitine Palmitoyl Transferase Deficiency I
PathBank:SMP0000542 Medium Chain Acyl-CoA Dehydrogenase Deficiency (MCAD)
PathBank:SMP0000558 Primary Hyperoxaluria II, PH2
PathBank:SMP0002355 Pyruvate Metabolism
PathBank:SMP0087327 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0087359 Mitochondrial Beta-Oxidation of Medium Chain Saturated Fatty Acids
PathBank:SMP0063639 Mitochondrial Beta-Oxidation of Long Chain Saturated Fatty Acids