EC: 2.8.2.21

keratan sulfotransferase (3'-phosphoadenylyl-sulfate:keratan 6'-sulfotransferase)

enzyme lambda metabolic reaction experiment
uniprot:O43916 [keratan sulfotransferase(Enzyme) wildtype]

NeuAc_alpha2_>3Gal_beta1_>4GlcNAc(6_sulfate)_beta1_>3Gal(6_sulfate)_beta1_>4GlcNAc_6_sulfate + 3'_Phosphoadenylyl_sulfate -> Adenosine_3',5'_bisphosphate + NeuAc_alpha2_>3Gal(6_sulfate)_beta1_>4GlcNAc(6_sulfate)_beta1_>3Gal(6_sulfate)_beta1_>4GlcNAc_6_sulfate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Imidazole HCl, 10 mM CaCl2, 2 mM dithiothreitol
PH: 6.4
Temperature: 37
uniprot:O43916 [keratan sulfotransferase(Enzyme) wildtype]

3'_Phosphoadenylyl_sulfate + Gal_beta1_>4GlcNAc(6_sulfate)_beta1_>3Gal(6_sulfate)_beta1_>4GlcNAc_6_sulfate -> Adenosine_3',5'_bisphosphate + 6_Sulfo_Gal_beta1_>4GlcNAc(6_sulfo)beta1_3Gal(6_sulfo)beta1_4GlcNAc(6_sulfo)		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Imidazole HCl, 10 mM CaCl2, 2 mM dithiothreitol
PH: 6.4
Temperature: 37
uniprot:O43916 [keratan sulfotransferase(Enzyme) wildtype]

3'_Phosphoadenylyl_sulfate + Keratan_sulfate -> Keratan_sulfate + Adenosine_3',5'_bisphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Imidazole HCl, 10 mM CaCl2, 2 mM dithiothreitol
PH: 6.4
Temperature: 37
uniprot:O43916 [keratan sulfotransferase(Enzyme) wildtype]

3'_Phosphoadenylyl_sulfate + Keratan_sulfate -> Adenosine_3',5'_bisphosphate + Keratan_sulfate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Imidazole HCl, 10 mM CaCl2, 2 mM dithiothreitol
PH: 6.4
Temperature: 37
uniprot:O43916 [ATP]

Keratan_sulfate + 3'_Phosphoadenylyl_sulfate -> Adenosine_3',5'_bisphosphate + Keratan_sulfate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Bis-Tris propane, 10 mM NaF, 0.1% Triton X-100, 10% Glycerol
PH: 7.5
Temperature: 22

Pathways

pathway id name
BioCyc:HUMAN_PWY-7831 biosynthesis of ABH and Lewis epitopes from type 2 precursor disaccharide
BioCyc:META_PWY-7831 ABH and Lewis epitopes biosynthesis from type 2 precursor disaccharide
BioCyc:HUMAN_PWY-6567 chondroitin sulfate biosynthesis (late stages)
BioCyc:META_PWY-6567 chondroitin sulfate biosynthesis (late stages)
Reactome:R-HSA-1630316 Glycosaminoglycan metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-1630316 Glycosaminoglycan metabolism
Reactome:R-DRE-2022854 Keratan sulfate biosynthesis
Reactome:R-HSA-71387 Carbohydrate metabolism
Reactome:R-RNO-2022854 Keratan sulfate biosynthesis
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-1630316 Glycosaminoglycan metabolism
Reactome:R-HSA-1638074 Keratan sulfate/keratin metabolism
Reactome:R-MMU-71387 Carbohydrate metabolism
Reactome:R-MMU-1638074 Keratan sulfate/keratin metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-1630316 Glycosaminoglycan metabolism
Reactome:R-DRE-71387 Carbohydrate metabolism
Reactome:R-DRE-1638074 Keratan sulfate/keratin metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2022854 Keratan sulfate biosynthesis
Reactome:R-MMU-2022854 Keratan sulfate biosynthesis
Reactome:R-RNO-71387 Carbohydrate metabolism
Reactome:R-RNO-1638074 Keratan sulfate/keratin metabolism