EC: 2.7.7.40

D-ribitol-5-phosphate cytidylyltransferase (CTP:D-ribitol-5-phosphate cytidylyltransferase)

enzyme lambda metabolic reaction experiment
- [D-ribitol-5-phosphate cytidylyltransferase(Enzyme) wildtype TarIJ]

D_Ribitol_5_phosphate + CTP -> Diphosphate + CDPribitol		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (( (KiA * KmB) ) + (KmA * B)) ) + (KmB * A)) ) + (A * B))) buffer: 25 mM HEPES, 1 mM Dithiothreitol, 10 mM MgCl2, 0.01% Triton X-100
PH: 7.2
Temperature: 22
- [D-ribitol-5-phosphate cytidylyltransferase(Enzyme) wildtype Bcs1]

D_Ribitol_5_phosphate + CTP -> Diphosphate + CDPribitol		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (( (KiA * KmB) ) + (KmA * B)) ) + (KmB * A)) ) + (A * B))) buffer: 25 mM HEPES, 1 mM Dithiothreitol, 85 µM CTP, 10 mM MgCl2, 0.01% Triton X-100
PH: 7.2
Temperature: 22
- [D-ribitol-5-phosphate cytidylyltransferase(Enzyme) wildtype Bcs1]

CTP + D_Ribitol_5_phosphate -> Diphosphate + CDPribitol		 (( (kcat * E) ) / (( (( (Km / A) ) * (1 + (I / Ki))) ) + (1 + (I / alphaKi)))) buffer: 25 mM HEPES, 1 mM Dithiothreitol, 85 µM CTP, 10 mM MgCl2, 0.01% Triton X-100
PH: 7.2
Temperature: 22
- [D-ribitol-5-phosphate cytidylyltransferase(Enzyme) wildtype Bcs1]

D_Ribitol_5_phosphate + CTP -> Diphosphate + CDPribitol		 (( (kcat * E) ) / (( (( (Km / A) ) * (1 + (I / Ki))) ) + (1 + (I / alphaKi)))) buffer: 25 mM HEPES, 1 mM Dithiothreitol, 85 µM CTP, 10 mM MgCl2, 0.01% Triton X-100
PH: 7.2
Temperature: 22
- [D-ribitol-5-phosphate cytidylyltransferase(Enzyme) wildtype TarIJ]

D_Ribitol_5_phosphate + CTP -> CDPribitol + Diphosphate		 (( (kcat * E) ) / (( (( (Km / A) ) * (1 + (I / Ki))) ) + (1 + (I / alphaKi)))) buffer: 25 mM HEPES, 1 mM Dithiothreitol, 10 mM MgCl2, 0.01% Triton X-100
PH: 7.2
Temperature: 22
- [D-ribitol-5-phosphate cytidylyltransferase(Enzyme) wildtype Bcs1]

CTP + D_Ribitol_5_phosphate -> Diphosphate + CDPribitol		 (( (kcat * E) ) / (( (( (Km / A) ) * (1 + (I / Ki))) ) + (1 + (I / alphaKi)))) buffer: 25 mM HEPES, 1 mM Dithiothreitol, 85 µM CTP, 10 mM MgCl2, 0.01% Triton X-100
PH: 7.2
Temperature: 22
- [D-ribitol-5-phosphate cytidylyltransferase(Enzyme) wildtype TarIJ]

D_Ribitol_5_phosphate + CTP -> CDPribitol + Diphosphate		 (( (kcat * E) ) / (( (( (Km / A) ) * (1 + (I / Ki))) ) + (1 + (I / alphaKi)))) buffer: 25 mM HEPES, 1 mM Dithiothreitol, 10 mM MgCl2, 0.01% Triton X-100
PH: 7.2
Temperature: 22
- [D-ribitol-5-phosphate cytidylyltransferase(Enzyme) wildtype Bcs1]

D_Ribitol_5_phosphate + CTP -> Diphosphate + CDPribitol		 (( (kcat * E) ) / (1 + (( (Km / A) ) * (1 + (I / Ki))))) buffer: 25 mM HEPES, 1 mM Dithiothreitol, 85 µM CTP, 10 mM MgCl2, 0.01% Triton X-100
PH: 7.2
Temperature: 22
- [D-ribitol-5-phosphate cytidylyltransferase(Enzyme) wildtype TarIJ]

D_Ribitol_5_phosphate + CTP -> Diphosphate + CDPribitol		 (( (kcat * E) ) / (1 + (( (Km / A) ) * (1 + (I / Ki))))) buffer: 25 mM HEPES, 1 mM Dithiothreitol, 10 mM MgCl2, 0.01% Triton X-100
PH: 7.2
Temperature: 22
- [D-ribitol-5-phosphate cytidylyltransferase(Enzyme) wildtype TarIJ]

D_Ribitol_5_phosphate + CTP -> CDPribitol + Diphosphate		 (( (kcat * E) ) / (( (( (Km / A) ) * (1 + (I / Ki))) ) + (1 + (I / alphaKi)))) buffer: 25 mM HEPES, 1 mM Dithiothreitol, 10 mM MgCl2, 0.01% Triton X-100
PH: 7.2
Temperature: 22
uniprot:Q48230 [D-ribitol-5-phosphate cytidylyltransferase(Enzyme) wildtype]

D_Ribitol_5_phosphate + CTP -> Diphosphate + CDPribitol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM HEPES, 1 mM DTT, 0.01% Triton X-100, 10 mM MgCl2, 200 µM MESG, 2 units/ml Purine ribonucleoside phosphorylase, 20 units/ml Inorganic pyrophosphatase
PH: 7.2
Temperature: 25
uniprot:Q48230 [D-ribitol-5-phosphate cytidylyltransferase(Enzyme) wildtype]

D_Ribitol_5_phosphate + CTP -> Diphosphate + CDPribitol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM HEPES, 1 mM DTT, 0.01% Triton X-100, 10 mM MgCl2, 200 µM MESG, 2 units/ml Purine ribonucleoside phosphorylase, 20 units/ml Inorganic pyrophosphatase
PH: 7.2
Temperature: 25
uniprot:Q48230 [D-ribitol-5-phosphate cytidylyltransferase(Enzyme) wildtype His-tagged]

CTP + D_Ribitol_5_phosphate -> Diphosphate + CDPribitol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM HEPES, 1 mM DTT, 0.01% Triton X-100, 10 mM MgCl2, 200 µM MESG, 2 units/ml Purine ribonucleoside phosphorylase, 20 units/ml Inorganic pyrophosphatase
PH: 7.2
Temperature: 25
uniprot:Q48230 [D-ribitol-5-phosphate cytidylyltransferase(Enzyme) wildtype His-tagged]

D_Ribitol_5_phosphate + CTP -> Diphosphate + CDPribitol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM HEPES, 1 mM DTT, 0.01% Triton X-100, 10 mM MgCl2, 200 µM MESG, 2 units/ml Purine ribonucleoside phosphorylase, 20 units/ml Inorganic pyrophosphatase
PH: 7.2
Temperature: 25
uniprot:Q48230 [D-ribitol-5-phosphate cytidylyltransferase(Enzyme) mutant R18A His-tagged]

D_Ribitol_5_phosphate + CTP -> CDPribitol + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM HEPES, 1 mM DTT, 0.01% Triton X-100, 10 mM MgCl2, 200 µM MESG, 2 units/ml Purine ribonucleoside phosphorylase, 20 units/ml Inorganic pyrophosphatase
PH: 7.2
Temperature: 25
uniprot:Q48230 [D-ribitol-5-phosphate cytidylyltransferase(Enzyme) mutant K386A His-tagged]

CTP + D_Ribitol_5_phosphate -> CDPribitol + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM HEPES, 1 mM DTT, 0.01% Triton X-100, 10 mM MgCl2, 200 µM MESG, 2 units/ml Purine ribonucleoside phosphorylase, 20 units/ml Inorganic pyrophosphatase
PH: 7.2
Temperature: 25
uniprot:Q48230 [D-ribitol-5-phosphate cytidylyltransferase(Enzyme) mutant K386A His-tagged]

CTP + D_Ribitol_5_phosphate -> CDPribitol + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM HEPES, 1 mM DTT, 0.01% Triton X-100, 10 mM MgCl2, 200 µM MESG, 2 units/ml Purine ribonucleoside phosphorylase, 20 units/ml Inorganic pyrophosphatase
PH: 7.2
Temperature: 25
uniprot:Q48230 [D-ribitol-5-phosphate cytidylyltransferase(Enzyme) mutant R18A His-tagged]

CTP + D_Ribitol_5_phosphate -> CDPribitol + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM HEPES, 1 mM DTT, 0.01% Triton X-100, 10 mM MgCl2, 200 µM MESG, 2 units/ml Purine ribonucleoside phosphorylase, 20 units/ml Inorganic pyrophosphatase
PH: 7.2
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY-7981 α-dystroglycan glycosylation
BioCyc:HUMAN_PWY-7981 α-dystroglycan glycosylation
Reactome:R-RNO-8931838 DAG1 glycosylations
Reactome:R-RNO-9939291 Matriglycan biosynthesis on DAG1
Reactome:R-HSA-8931838 DAG1 glycosylations
Reactome:R-HSA-9939291 Matriglycan biosynthesis on DAG1
Reactome:R-DRE-8931838 DAG1 glycosylations
Reactome:R-DRE-9939291 Matriglycan biosynthesis on DAG1
Reactome:R-MMU-8931838 DAG1 glycosylations
Reactome:R-MMU-9939291 Matriglycan biosynthesis on DAG1
Reactome:R-XTR-8931838 DAG1 glycosylations
Reactome:R-XTR-9939291 Matriglycan biosynthesis on DAG1
Reactome:R-DRE-392499 Metabolism of proteins
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-HSA-5173105 O-linked glycosylation
Reactome:R-RNO-597592 Post-translational protein modification
Reactome:R-RNO-5173105 O-linked glycosylation
Reactome:R-RNO-392499 Metabolism of proteins
Reactome:R-XTR-597592 Post-translational protein modification
Reactome:R-XTR-5173105 O-linked glycosylation
Reactome:R-DRE-5173105 O-linked glycosylation
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-MMU-5173105 O-linked glycosylation
Reactome:R-XTR-392499 Metabolism of proteins
Reactome:R-DRE-597592 Post-translational protein modification