EC: 2.7.1.36

mevalonate kinase (ATP:(R)-mevalonate 5-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q03426 [Isopentenyl diphosphate]

ATP + Mevalonate -> ADP + Mevalonate_5_phosphate		 (( (V * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM MOPS-KOH, 6 mM MgCl2, 10 mM KF, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml pyruvate kinase, 10 U/ml lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:Q03426 [mevalonate kinase(Enzyme) wildtype]

Mevalonate + ATP -> Mevalonate_5_phosphate + ADP		 (( (V * B) ) / (Km + B)) buffer: 50 mM MOPS-KOH, 6 mM MgCl2, 10 mM KF, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml pyruvate kinase, 10 U/ml lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:Q03426 [mevalonate kinase(Enzyme) wildtype]

ATP + Mevalonate -> ADP + Mevalonate_5_phosphate		 (( (V * A) ) / (Km + A)) buffer: 50 mM MOPS-KOH, 6 mM MgCl2, 10 mM KF, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml pyruvate kinase, 10 U/ml lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:Q03426 [trans,trans-Farnesyl diphosphate]

ATP + Mevalonate -> Mevalonate_5_phosphate + ADP		 (( (V * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM MOPS-KOH, 6 mM MgCl2, 10 mM KF, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml pyruvate kinase, 10 U/ml lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:Q03426 [Dimethylallyl diphosphate]

Mevalonate + ATP -> ADP + Mevalonate_5_phosphate		 (( (V * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM MOPS-KOH, 6 mM MgCl2, 10 mM KF, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml pyruvate kinase, 10 U/ml lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:Q03426 [Geranylgeranyl diphosphate]

ATP + Mevalonate -> ADP + Mevalonate_5_phosphate		 (( (V * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM MOPS-KOH, 6 mM MgCl2, 10 mM KF, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml pyruvate kinase, 10 U/ml lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:Q03426 [Geranyl diphosphate]

ATP + Mevalonate -> ADP + Mevalonate_5_phosphate		 (( (V * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM MOPS-KOH, 6 mM MgCl2, 10 mM KF, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml pyruvate kinase, 10 U/ml lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:Q03426 [Farnesol]

ATP + Mevalonate -> ADP + Mevalonate_5_phosphate		 (( (V * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM MOPS-KOH, 6 mM MgCl2, 10 mM KF, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml pyruvate kinase, 10 U/ml lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:Q03426 [Dolichyl phosphate]

Mevalonate + ATP -> ADP + Mevalonate_5_phosphate		 (( (V * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM MOPS-KOH, 6 mM MgCl2, 10 mM KF, 1 mM phosphoenolpyruvate, 0.2 mM NADH, 50 U/ml pyruvate kinase, 50 U/ml lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:Q58487 [mevalonate kinase(Enzyme) wildtype His-tagged]

ATP + Mevalonate -> Mevalonate_5_phosphate + ADP		 (( (V * A) ) / (Km + A)) buffer: 0.1 M KH2PO4/K2HPO4, 0.16 mM NADH, 5 mM MgCl2, 0.5 mM phosphoenolpyruvate, 81 U/ml pyruvate kinase, 75 U/ml lactate dehydrogenase
PH: 8
Temperature: 70
uniprot:Q58487 [mevalonate kinase(Enzyme) wildtype His-tagged]

Mevalonate + ATP -> ADP + Mevalonate_5_phosphate		 (( (V * B) ) / (Km + B)) buffer: 0.1 M KH2PO4/K2HPO4, 0.16 mM NADH, 5 mM MgCl2, 0.5 mM phosphoenolpyruvate, 81 U/ml pyruvate kinase, 75 U/ml lactate dehydrogenase
PH: 8
Temperature: 70
uniprot:Q03426 [mevalonate kinase(Enzyme) wildtype]

ATP + Mevalonate -> ADP + Mevalonate_5_phosphate		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 10 mM MgCl2
PH: 7
Temperature: 30
uniprot:Q03426 [mevalonate kinase(Enzyme) wildtype]

ATP + Mevalonate -> ADP + Mevalonate_5_phosphate		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 10 mM MgCl2
PH: 7
Temperature: 30
uniprot:Q03426 [mevalonate kinase(Enzyme) mutant E193Q]

Mevalonate + ATP -> Mevalonate_5_phosphate + ADP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 10 mM MgCl2
PH: 7
Temperature: 30
uniprot:Q03426 [mevalonate kinase(Enzyme) mutant D204N]

ATP + Mevalonate -> ADP + Mevalonate_5_phosphate		 (( (V * B) ) / (Km + B)) buffer: 25 mM Tris, 60 mM MgCl2, 0.5 mM EDTA, 2.5 mM dithiothreitol, 125 mM sucrose, 383 mM NaF
PH: 7
Temperature: 37
uniprot:Q03426 [mevalonate kinase(Enzyme) mutant E19D]

ATP + Mevalonate -> Mevalonate_5_phosphate + ADP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 10 mM MgCl2
PH: 7
Temperature: 30
uniprot:Q03426 [mevalonate kinase(Enzyme) mutant E296Q]

ATP + Mevalonate -> ADP + Mevalonate_5_phosphate		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 10 mM MgCl2
PH: 7
Temperature: 30
uniprot:Q03426 [mevalonate kinase(Enzyme) mutant D204A]

ATP + Mevalonate -> ADP + Mevalonate_5_phosphate		 (( (V * A) ) / (Km + A)) buffer: 25 mM Tris, 60 mM MgCl2, 0.5 mM EDTA, 2.5 mM dithiothreitol, 125 mM sucrose, 383 mM NaF
PH: 7
Temperature: 37
uniprot:Q03426 [mevalonate kinase(Enzyme) mutant D204N]

ATP + Mevalonate -> Mevalonate_5_phosphate + ADP		 (( (V * A) ) / (Km + A)) buffer: 25 mM Tris, 60 mM MgCl2, 0.5 mM EDTA, 2.5 mM dithiothreitol, 125 mM sucrose, 383 mM NaF
PH: 7
Temperature: 37
uniprot:Q03426 [mevalonate kinase(Enzyme) mutant E19D]

ATP + Mevalonate -> Mevalonate_5_phosphate + ADP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 10 mM MgCl2
PH: 7
Temperature: 30
uniprot:Q03426 [mevalonate kinase(Enzyme) mutant E193Q]

ATP + Mevalonate -> ADP + Mevalonate_5_phosphate		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 10 mM MgCl2
PH: 7
Temperature: 30
uniprot:Q03426 [mevalonate kinase(Enzyme) mutant E296Q]

Mevalonate + ATP -> Mevalonate_5_phosphate + ADP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 10 mM MgCl2
PH: 7
Temperature: 30
uniprot:Q03426 [mevalonate kinase(Enzyme) mutant D204A]

Mevalonate + ATP -> Mevalonate_5_phosphate + ADP		 (( (V * B) ) / (Km + B)) buffer: 25 mM Tris, 60 mM MgCl2, 0.5 mM EDTA, 2.5 mM dithiothreitol, 125 mM sucrose, 383 mM NaF
PH: 7
Temperature: 37
- [mevalonate kinase(Enzyme) wildtype]

(R)_Mevalonate + ATP -> (R)_5_Phosphomevalonate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 20 mM MgCl2, 10 mM DTT, 5 mM p-Coumarate
PH: 7.4
Temperature: 37
- [mevalonate kinase(Enzyme) wildtype]

(R)_Mevalonate + ATP -> ADP + (R)_5_Phosphomevalonate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 20 mM MgCl2, 10 mM DTT, 5 mM p-Coumarate
PH: 7.4
Temperature: 37
uniprot:P17256 [mevalonate kinase(Enzyme) mutant K13M]

Mevalonate + ATP -> ADP + Mevalonate_5_phosphate		 (( (V * A) ) / (Km + A)) buffer: 5 mM MgCl2
PH: 7
Temperature: 30
uniprot:P17256 [mevalonate kinase(Enzyme) wildtype]

ATP + Mevalonate -> Mevalonate_5_phosphate + ADP		 (( (V * A) ) / (Km + A)) buffer: 5 mM MgCl2
PH: 7
Temperature: 30
uniprot:P17256 [mevalonate kinase(Enzyme) mutant K13M]

ATP + Mevalonate -> ADP + Mevalonate_5_phosphate		 (( (V * A) ) / (Km + A)) buffer: 5 mM MgCl2
PH: 7
Temperature: 30
uniprot:P17256 [mevalonate kinase(Enzyme) wildtype]

Mevalonate + ATP -> ADP + Mevalonate_5_phosphate		 (( (V * A) ) / (Km + A)) buffer: 5 mM MgCl2
PH: 7
Temperature: 30
uniprot:P17256 [mevalonate kinase(Enzyme) wildtype]

ATP + Mevalonate -> ADP + Mevalonate_5_phosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM Potassium phosphate, 10 mM KF, 10 mM MgCl2, 10 mM DTT, 0.5 mM NADH, 1 mM Phsophoenolpyruvate, 20 U/ml Pyruvate kinase, 27 U/ml Lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:P17256 [mevalonate kinase(Enzyme) wildtype]

Mevalonate + ATP -> Mevalonate_5_phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Potassium phosphate, 10 mM KF, 10 mM MgCl2, 10 mM DTT, 0.5 mM NADH, 1 mM Phsophoenolpyruvate, 20 U/ml Pyruvate kinase, 27 U/ml Lactate dehydrogenase
PH: 7
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY-922 mevalonate pathway I
BioCyc:MOUSE_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:HUMAN_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:HUMAN_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
BioCyc:YEAST_ERGOSTEROL-SYN-PWY superpathway of ergosterol biosynthesis
BioCyc:YEAST_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
BioCyc:YEAST_PWY-922 mevalonate pathway
BioCyc:META_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
BioCyc:META_PWY-6174 mevalonate pathway II (archaea)
BioCyc:META_PWY-7391 isoprene biosynthesis II (engineered)
BioCyc:HUMAN_PWY-922 mevalonate pathway
BioCyc:META_ERGOSTEROL-SYN-PWY superpathway of ergosterol biosynthesis I
BioCyc:META_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:MOUSE_PWY-922 mevalonate pathway I
PlantCyc:PLANT_PWY-922 mevalonate pathway I
PlantCyc:PLANT_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
Reactome:R-HSA-9969896 Lanosterol biosynthesis
Reactome:R-MMU-9969896 Lanosterol biosynthesis
Reactome:R-SPO-9969896 Lanosterol biosynthesis
Reactome:R-BTA-9969896 Lanosterol biosynthesis
Reactome:R-DDI-9969896 Lanosterol biosynthesis
Reactome:R-RNO-9969896 Lanosterol biosynthesis
Reactome:R-SCE-9969896 Lanosterol biosynthesis
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-BTA-191273 Cholesterol biosynthesis
Reactome:R-DDI-191273 Cholesterol biosynthesis
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-SCE-8957322 Metabolism of steroids
Reactome:R-SPO-1430728 Metabolism
Reactome:R-BTA-8957322 Metabolism of steroids
Reactome:R-DDI-8957322 Metabolism of steroids
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-191273 Cholesterol biosynthesis
Reactome:R-HSA-2426168 Activation of gene expression by SREBF (SREBP)
Reactome:R-SCE-1430728 Metabolism
Reactome:R-MMU-191273 Cholesterol biosynthesis
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-8957322 Metabolism of steroids
Reactome:R-SCE-556833 Metabolism of lipids
Reactome:R-SCE-191273 Cholesterol biosynthesis
Reactome:R-SPO-8957322 Metabolism of steroids
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-556833 Metabolism of lipids
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-1655829 Regulation of cholesterol biosynthesis by SREBP (SREBF)
Reactome:R-RNO-191273 Cholesterol biosynthesis
Reactome:R-SPO-556833 Metabolism of lipids
Reactome:R-SPO-191273 Cholesterol biosynthesis
WikiPathways:WP4190 Mevalonate arm of cholesterol biosynthesis pathway
PathBank:SMP0120794 Mevalonic Aciduria
PathBank:SMP0000023 Steroid Biosynthesis
PathBank:SMP0000387 CHILD Syndrome
PathBank:SMP0000112 Risedronate Action Pathway
PathBank:SMP0000508 Cholesteryl Ester Storage Disease
PathBank:SMP0002438 Cholesterol Biosynthesis and Metabolism CE(12:0)
PathBank:SMP0087326 Steroid Biosynthesis
PathBank:SMP0120685 Chondrodysplasia Punctata II, X-Linked Dominant (CDPX2)
PathBank:SMP0120769 Hypercholesterolemia
PathBank:SMP0120793 Hyper-IgD Syndrome
PathBank:SMP0121055 Mevalonate Pathway
PathBank:SMP0000389 Smith-Lemli-Opitz Syndrome (SLOS)
PathBank:SMP0000319 Lysosomal Acid Lipase Deficiency (Wolman Disease)
PathBank:SMP0000082 Simvastatin Action Pathway
PathBank:SMP0000111 Cerivastatin Action Pathway
PathBank:SMP0000131 Atorvastatin Action Pathway
PathBank:SMP0000511 Wolman Disease
PathBank:SMP0002381 Steroid Biosynthesis
PathBank:SMP0002436 Cholesterol biosynthesis and metabolism CE(10:0)
PathBank:SMP0002441 Cholesterol biosynthesis and metabolism CE(18:0)
PathBank:SMP0087233 Steroid Biosynthesis
PathBank:SMP0120684 CHILD Syndrome
PathBank:SMP0120692 Desmosterolosis
PathBank:SMP0120732 Lysosomal Acid Lipase Deficiency (Wolman Disease)
PathBank:SMP0120792 Cholesteryl Ester Storage Disease
PathBank:SMP0070041 Cholesterol Biosynthesis and Metabolism
PathBank:SMP0000386 Desmosterolosis
PathBank:SMP0000089 Pravastatin Action Pathway
PathBank:SMP0000095 Alendronate Action Pathway
PathBank:SMP0000099 Lovastatin Action Pathway
PathBank:SMP0000117 Pamidronate Action Pathway
PathBank:SMP0000509 Hyper-IgD Syndrome
PathBank:SMP0120735 Smith-Lemli-Opitz Syndrome (SLOS)
PathBank:SMP0120795 Wolman Disease
PathBank:SMP0121209 Mevalonate Pathway
PathBank:SMP0000388 Chondrodysplasia Punctata II, X-Linked Dominant (CDPX2)
PathBank:SMP0000079 Ibandronate Action Pathway
PathBank:SMP0000092 Rosuvastatin Action Pathway
PathBank:SMP0000209 Hypercholesterolemia
PathBank:SMP0000107 Zoledronate Action Pathway
PathBank:SMP0000119 Fluvastatin Action Pathway
PathBank:SMP0000510 Mevalonic Aciduria
PathBank:SMP0002384 Terpenoid Backbone Biosynthesis
PathBank:SMP0002435 Cholesterol biosynthesis and metabolism CE(14:0)
PathBank:SMP0002440 Cholesterol Biosynthesis and Metabolism CE(16:0)