EC: 1.1.1.34

hydroxymethylglutaryl-CoA reductase (NADPH) ((R)-mevalonate:NADP+ oxidoreductase (CoA-acylating))

enzyme lambda metabolic reaction experiment
- [hydroxymethylglutaryl-CoA reductase (NADPH)(Enzyme) wildtype]

2 NADPH + 3_Hydroxy_3_methylglutaryl_CoA + 2 H+ -> (R)_Mevalonate + 2 NADP+ + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 28.75 mM Potassium phosphate, 45 mM Glucose-6-phosphate, 4.5 mM NADP+, 0.25 unit/ml Glucose-6-phosphate dehydrogenase
PH: 7.5
Temperature: 37
- [hydroxymethylglutaryl-CoA reductase (NADPH)(Enzyme) wildtype]

3_Hydroxy_3_methylglutaryl_CoA + 2 H+ + 2 NADPH -> Coenzyme_A + 2 NADP+ + (R)_Mevalonate		 (( (Vmax * S) ) / (Km + S)) buffer: 28.75 mM Potassium phosphate, 45 mM Glucose-6-phosphate, 4.5 mM NADP+, 0.25 unit/ml Glucose-6-phosphate dehydrogenase
PH: 7.5
Temperature: 37
- [3-Hydroxy-3-methylglutarate]

2 NAD+ + Mevalonate + Coenzyme_A -> 3_Hydroxy_3_methylglutaryl_CoA + 2 NADH + 2 H+		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris-HCl, 100 mM KCl
PH: 8
Temperature: 37
- [Acetoacetate]

Coenzyme_A + 2 NAD+ + Mevalonate -> 2 NADH + 3_Hydroxy_3_methylglutaryl_CoA + 2 H+		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris-HCl, 100 mM KCl
PH: 8
Temperature: 37
- [2-Hydroxybutyrate]

Coenzyme_A + Mevalonate + 2 NAD+ -> 3_Hydroxy_3_methylglutaryl_CoA + 2 H+ + 2 NADH		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris-HCl, 100 mM KCl
PH: 8
Temperature: 37
- [3-Hydroxybutyrate]

2 NAD+ + Mevalonate + Coenzyme_A -> 2 H+ + 3_Hydroxy_3_methylglutaryl_CoA + 2 NADH		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris-HCl, 100 mM KCl
PH: 8
Temperature: 37
- [Lovastatin]

2 H+ + 3_Hydroxy_3_methylglutaryl_CoA + 2 NADH -> Coenzyme_A + 2 NAD+ + Mevalonate		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM KxPO4, 100 mM KCl
PH: 6.5
Temperature: 37
- [Lovastatin]

3_Hydroxy_3_methylglutaryl_CoA + 2 NADH + 2 H+ -> Coenzyme_A + Mevalonate + 2 NAD+		 (( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + (A * (1 + (I / Ki))))) buffer: 100 mM KxPO4, 100 mM KCl
PH: 6.5
Temperature: 37
- [Lovastatin]

Mevalonate + 2 NAD+ + Coenzyme_A -> 3_Hydroxy_3_methylglutaryl_CoA + 2 H+ + 2 NADH		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris-HCl, 100 mM KCl
PH: 8
Temperature: 37
- [Lovastatin]

2 NAD+ + Mevalonate + Coenzyme_A -> 2 H+ + 3_Hydroxy_3_methylglutaryl_CoA + 2 NADH		 (( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + (A * (1 + (I / Ki))))) buffer: 100 mM Tris-HCl, 100 mM KCl
PH: 8
Temperature: 37
uniprot:O15888 [Lovastatin]

2 H+ + 2 NADPH + 3_Hydroxy_3_methylglutaryl_CoA -> Coenzyme_A + (R)_Mevalonate + 2 NADP+		 (( (Vmax * S1) ) / (( (Km1 * (1 + (I / Ki_S1))) ) + S1)) buffer: 30 mM EDTA, 70 mM NaCl, 10 mM beta-Mercaptoethanol, 30 mM Glucose 6-phosphate, 2.0 U/ml Glucose 6-phosphate dehydrogenase, 0.1 % Triton X-100
PH: 6.8
Temperature: 30
uniprot:O15888 [hydroxymethylglutaryl-CoA reductase (NADPH)(Enzyme) wildtype]

2 H+ + 3_Hydroxy_3_methylglutaryl_CoA + 2 NADPH -> (R)_Mevalonate + 2 NADP+ + Coenzyme_A		 (( (Vmax * S1) ) / (Km1 + S1)) buffer: 30 mM EDTA, 70 mM NaCl, 10 mM beta-Mercaptoethanol, 30 mM Glucose 6-phosphate, 2.0 U/ml Glucose 6-phosphate dehydrogenase, 0.1 % Triton X-100
PH: 6.8
Temperature: 30
uniprot:O15888 [hydroxymethylglutaryl-CoA reductase (NADPH)(Enzyme) wildtype]

2 NADPH + 2 H+ + 3_Hydroxy_3_methylglutaryl_CoA -> Coenzyme_A + 2 NADP+ + (R)_Mevalonate		 (( (Vmax * S2) ) / (Km2 + S2)) buffer: 30 mM EDTA, 70 mM NaCl, 10 mM beta-Mercaptoethanol, 30 mM Glucose 6-phosphate, 2.0 U/ml Glucose 6-phosphate dehydrogenase, 0.1 % Triton X-100
PH: 6.8
Temperature: 30
uniprot:O15888 [Lovastatin]

2 H+ + 3_Hydroxy_3_methylglutaryl_CoA + 2 NADPH -> 2 NADP+ + Coenzyme_A + (R)_Mevalonate		 (( (Vmax * S2) ) / (( (Km2 + S2) ) * (1 + (I / Ki_S2)))) buffer: 30 mM EDTA, 70 mM NaCl, 10 mM beta-Mercaptoethanol, 30 mM Glucose 6-phosphate, 2.0 U/ml Glucose 6-phosphate dehydrogenase, 0.1 % Triton X-100
PH: 6.8
Temperature: 30
uniprot:P04035 [hydroxymethylglutaryl-CoA reductase (NADPH)(Enzyme) wildtype]

3_Hydroxy_3_methylglutaryl_CoA + 2 H+ + 2 NADPH -> Coenzyme_A + (R)_Mevalonate + 2 NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 20 mM glucose-6-phosphate, 3.5 U/ml glucose 6-phosphate dehydrogenase, 10 mM sodium EDTA, 5 mM DTT
PH: 7.4
Temperature: 37
uniprot:Q01237 [hydroxymethylglutaryl-CoA reductase (NADPH)(Enzyme) wildtype]

2 H+ + 2 NADPH + 3_Hydroxy_3_methylglutaryl_CoA -> Coenzyme_A + 2 NADP+ + (R)_Mevalonate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 20 mM glucose-6-phosphate, 3.5 U/ml glucose 6-phosphate dehydrogenase, 10 mM sodium EDTA, 5 mM DTT
PH: 7.4
Temperature: 37
uniprot:Q01237 [hydroxymethylglutaryl-CoA reductase (NADPH)(Enzyme) wildtype]

3_Hydroxy_3_methylglutaryl_CoA + 2 NADPH + 2 H+ -> Coenzyme_A + (R)_Mevalonate + 2 NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 20 mM glucose-6-phosphate, 3.5 U/ml glucose 6-phosphate dehydrogenase, 10 mM sodium EDTA, 5 mM DTT
PH: 7.4
Temperature: 37
uniprot:Q01237 [hydroxymethylglutaryl-CoA reductase (NADPH)(Enzyme) wildtype]

2 NADPH + 2 H+ + 3_Hydroxy_3_methylglutaryl_CoA -> (R)_Mevalonate + 2 NADP+ + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 20 mM glucose-6-phosphate, 3.5 U/ml glucose 6-phosphate dehydrogenase, 10 mM sodium EDTA, 5 mM DTT
PH: 7.4
Temperature: 37
uniprot:Q01237 [hydroxymethylglutaryl-CoA reductase (NADPH)(Enzyme) wildtype]

3_Hydroxy_3_methylglutaryl_CoA + 2 H+ + 2 NADPH -> Coenzyme_A + (R)_Mevalonate + 2 NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 20 mM glucose-6-phosphate, 3.5 U/ml glucose 6-phosphate dehydrogenase, 10 mM sodium EDTA, 5 mM DTT
PH: 7.4
Temperature: 37
uniprot:P04035 [hydroxymethylglutaryl-CoA reductase (NADPH)(Enzyme) wildtype]

2 H+ + 3_Hydroxy_3_methylglutaryl_CoA + 2 NADPH -> (R)_Mevalonate + 2 NADP+ + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M potassium phosphate, 20 mM glucose-6-phosphate, 3.5 U/ml glucose 6-phosphate dehydrogenase, 10 mM sodium EDTA, 5 mM DTT
PH: 7.4
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-922 mevalonate pathway I
BioCyc:MOUSE_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:HUMAN_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:HUMAN_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
BioCyc:YEAST_ERGOSTEROL-SYN-PWY superpathway of ergosterol biosynthesis
BioCyc:YEAST_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
BioCyc:YEAST_PWY-922 mevalonate pathway
BioCyc:ARA_PWY-922 mevalonate pathway I
BioCyc:META_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
BioCyc:META_PWY-6174 mevalonate pathway II (archaea)
BioCyc:HUMAN_PWY-922 mevalonate pathway
BioCyc:META_ERGOSTEROL-SYN-PWY superpathway of ergosterol biosynthesis I
BioCyc:META_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:MOUSE_PWY-922 mevalonate pathway I
PlantCyc:ARA_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
PlantCyc:PLANT_PWY-922 mevalonate pathway I
PlantCyc:PLANT_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
PlantCyc:ARA_PWY-922 mevalonate pathway I
Reactome:R-HSA-9619665 EGR2 and SOX10-mediated initiation of Schwann cell myelination
Reactome:R-HSA-9675108 Nervous system development
Reactome:R-DME-9969896 Lanosterol biosynthesis
Reactome:R-MMU-9969896 Lanosterol biosynthesis
Reactome:R-SPO-9969896 Lanosterol biosynthesis
Reactome:R-BTA-9969896 Lanosterol biosynthesis
Reactome:R-DDI-9969896 Lanosterol biosynthesis
Reactome:R-RNO-9969896 Lanosterol biosynthesis
Reactome:R-SCE-9969896 Lanosterol biosynthesis
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-BTA-191273 Cholesterol biosynthesis
Reactome:R-DDI-191273 Cholesterol biosynthesis
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-SCE-8957322 Metabolism of steroids
Reactome:R-SPO-1430728 Metabolism
Reactome:R-BTA-8957322 Metabolism of steroids
Reactome:R-DDI-8957322 Metabolism of steroids
Reactome:R-DME-1430728 Metabolism
Reactome:R-HSA-1266738 Developmental Biology
Reactome:R-SCE-1430728 Metabolism
Reactome:R-DME-8957322 Metabolism of steroids
Reactome:R-MMU-191273 Cholesterol biosynthesis
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-8957322 Metabolism of steroids
Reactome:R-SCE-556833 Metabolism of lipids
Reactome:R-SCE-191273 Cholesterol biosynthesis
Reactome:R-SPO-8957322 Metabolism of steroids
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-556833 Metabolism of lipids
Reactome:R-DME-556833 Metabolism of lipids
Reactome:R-DME-191273 Cholesterol biosynthesis
Reactome:R-RNO-191273 Cholesterol biosynthesis
Reactome:R-SPO-556833 Metabolism of lipids
Reactome:R-SPO-191273 Cholesterol biosynthesis
WikiPathways:WP4189 Mevalonate arm of cholesterol biosynthesis pathway with inhibitors
WikiPathways:WP5220 Metabolic reprogramming in pancreatic cancer
WikiPathways:WP4190 Mevalonate arm of cholesterol biosynthesis pathway
PathBank:SMP0000023 Steroid Biosynthesis
PathBank:SMP0000387 CHILD Syndrome
PathBank:SMP0000112 Risedronate Action Pathway
PathBank:SMP0000508 Cholesteryl Ester Storage Disease
PathBank:SMP0002438 Cholesterol Biosynthesis and Metabolism CE(12:0)
PathBank:SMP0121055 Mevalonate Pathway
PathBank:SMP0000389 Smith-Lemli-Opitz Syndrome (SLOS)
PathBank:SMP0000319 Lysosomal Acid Lipase Deficiency (Wolman Disease)
PathBank:SMP0000082 Simvastatin Action Pathway
PathBank:SMP0000111 Cerivastatin Action Pathway
PathBank:SMP0000131 Atorvastatin Action Pathway
PathBank:SMP0000511 Wolman Disease
PathBank:SMP0002381 Steroid Biosynthesis
PathBank:SMP0002436 Cholesterol biosynthesis and metabolism CE(10:0)
PathBank:SMP0002441 Cholesterol biosynthesis and metabolism CE(18:0)
PathBank:SMP0070041 Cholesterol Biosynthesis and Metabolism
PathBank:SMP0000386 Desmosterolosis
PathBank:SMP0000089 Pravastatin Action Pathway
PathBank:SMP0000095 Alendronate Action Pathway
PathBank:SMP0000099 Lovastatin Action Pathway
PathBank:SMP0000117 Pamidronate Action Pathway
PathBank:SMP0000509 Hyper-IgD Syndrome
PathBank:SMP0121209 Mevalonate Pathway
PathBank:SMP0000388 Chondrodysplasia Punctata II, X-Linked Dominant (CDPX2)
PathBank:SMP0000079 Ibandronate Action Pathway
PathBank:SMP0000092 Rosuvastatin Action Pathway
PathBank:SMP0000209 Hypercholesterolemia
PathBank:SMP0000107 Zoledronate Action Pathway
PathBank:SMP0000119 Fluvastatin Action Pathway
PathBank:SMP0000510 Mevalonic Aciduria
PathBank:SMP0002384 Terpenoid Backbone Biosynthesis
PathBank:SMP0002435 Cholesterol biosynthesis and metabolism CE(14:0)
PathBank:SMP0002440 Cholesterol Biosynthesis and Metabolism CE(16:0)