EC: 1.5.1.34

6,7-dihydropteridine reductase (5,6,7,8-tetrahydropteridine:NAD(P)+ oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:O76290 [6,7-dihydropteridine reductase(Enzyme) wildtype His-tagged]

NADPH + H+ + Quinonoid_6,7_dimethyl_dihydropterin -> 6,7_Dimethyl_tetrahydropterin + NADP+		 (( (E * kcat) ) / (( (1 + (Km / S)) ) + (S / Ksi))) buffer: 50 mM HEPES, 20 µg/ml horseradish peroxidase, 0.9 mM H2O2
PH: 7.4
Temperature: 25
uniprot:Q01782 [6,7-dihydropteridine reductase(Enzyme) wildtype His-tagged]

NADPH + H+ + Quinonoid_6,7_dimethyl_dihydropterin -> 6,7_Dimethyl_tetrahydropterin + NADP+		 (( (E * kcat) ) / (( (1 + (Km / S)) ) + (S / Ksi))) buffer: 50 mM HEPES, 20 µg/ml horseradish peroxidase, 0.9 mM H2O2
PH: 7.4
Temperature: 25
uniprot:O76290 [6,7-dihydropteridine reductase(Enzyme) wildtype His-tagged]

Quinonoid_7,8_dihydropterin + H+ + NADPH -> NADP+ + Tetrahydropterin		 (( (E * kcat) ) / (( (1 + (Km / S)) ) + (S / Ksi))) buffer: 50 mM HEPES, 20 µg/ml horseradish peroxidase, 0.9 mM H2O2
PH: 7.4
Temperature: 25
uniprot:Q01782 [6,7-dihydropteridine reductase(Enzyme) wildtype His-tagged]

Quinonoid_7,8_dihydropterin + NADPH + H+ -> Tetrahydropterin + NADP+		 (( (E * kcat) ) / (( (1 + (Km / S)) ) + (S / Ksi))) buffer: 50 mM HEPES, 20 µg/ml horseradish peroxidase, 0.9 mM H2O2
PH: 7.4
Temperature: 25
- [6,7-dihydropteridine reductase(Enzyme) wildtype His-tagged]

Quinonoid_6,7_dimethyl_dihydropterin + H+ + NADH -> 6,7_Dimethyl_tetrahydropterin + NAD+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 20 µg/ml horseradish peroxidase, 0.9 mM H2O2
PH: 7.4
Temperature: 25
- [6,7-dihydropteridine reductase(Enzyme) wildtype His-tagged]

H+ + Quinonoid_7,8_dihydropterin + NADH -> NAD+ + Tetrahydropterin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM HEPES, 20 µg/ml horseradish peroxidase, 0.9 mM H2O2
PH: 7.4
Temperature: 25
uniprot:P09417 [6,7-dihydropteridine reductase(Enzyme) wildtype]

Tetrahydrobiopterin + NAD+ -> H+ + NADH + 6,7_Dihydrobiopterin		 (( (V * S) ) / (Km + S)) buffer: Tris/HCl
PH: 7.2
Temperature: 37
uniprot:P09417 [6,7-dihydropteridine reductase(Enzyme) wildtype]

NAD+ + 2_Amino_4_hydroxy_6,7_dimethyltetrahydropteridine -> H+ + NADH + 2_Amino_4_hydroxy_6,7_dimethyldihydropteridine		 (( (V * S) ) / (Km + S)) buffer: Tris/HCl
PH: 7.2
Temperature: 37
uniprot:P09417 [6,7-dihydropteridine reductase(Enzyme) wildtype]

H+ + NADH + 2_Amino_4_hydroxy_6,7_dimethyldihydropteridine -> NAD+ + 2_Amino_4_hydroxy_6,7_dimethyltetrahydropteridine		 (( (V * S) ) / (Km + S)) buffer: Tris/HCl
PH: 7.2
Temperature: 37
uniprot:P09417 [6,7-dihydropteridine reductase(Enzyme) wildtype]

2_Amino_4_hydroxy_6,7_dimethyldihydropteridine + H+ + NADPH -> 2_Amino_4_hydroxy_6,7_dimethyltetrahydropteridine + NADP+		 (( (V * S) ) / (Km + S)) buffer: Tris/HCl
PH: 7.2
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PHENYLALANINE-DEG1-PWY phenylalanine degradation/tyrosine biosynthesis
BioCyc:META_PHENYLALANINE-DEG1-PWY L-phenylalanine degradation I (aerobic)
BioCyc:MOUSE_PWY3DJ-35549 Biopterin recycling
Reactome:R-BTA-8964208 Phenylalanine metabolism
Reactome:R-HSA-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-RNO-8964208 Phenylalanine metabolism
Reactome:R-BTA-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-RNO-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-DDI-8964208 Phenylalanine metabolism
Reactome:R-MMU-8964208 Phenylalanine metabolism
Reactome:R-DDI-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-MMU-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-HSA-8964208 Phenylalanine metabolism
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP4156 Biosynthesis and regeneration of tetrahydrobiopterin and catabolism of phenylalanine
WikiPathways:WP4259 Disorders of folate metabolism and transport
PathBank:SMP0120774 Hyperphenylalaninemia Due to DHPR-Deficiency
PathBank:SMP0000005 Pterine Biosynthesis
PathBank:SMP0000488 Hyperphenylalaninemia Due to 6-Pyruvoyltetrahydropterin Synthase Deficiency (ptps)
PathBank:SMP0087250 Pterine Biosynthesis
PathBank:SMP0120773 Hyperphenylalaninemia Due to 6-Pyruvoyltetrahydropterin Synthase Deficiency (ptps)
PathBank:SMP0000487 Hyperphenylalaninemia Due to Guanosine Triphosphate Cyclohydrolase Deficiency
PathBank:SMP0000491 Sepiapterin Reductase Deficiency
PathBank:SMP0120772 Hyperphenylalaninemia Due to Guanosine Triphosphate Cyclohydrolase Deficiency
PathBank:SMP0120776 Sepiapterin Reductase Deficiency
PathBank:SMP0000489 Hyperphenylalaninemia Due to DHPR-Deficiency
PathBank:SMP0120771 DOPA-Responsive Dystonia
PathBank:SMP0120775 Segawa Syndrome
PathBank:SMP0000486 DOPA-Responsive Dystonia
PathBank:SMP0000490 Segawa Syndrome
PathBank:SMP0087343 Pterine Biosynthesis