EC: 1.1.1.282

quinate/shikimate dehydrogenase [NAD(P)+] (L-quinate:NAD(P)+ 3-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) wildtype]

NAD+ + Quinate -> 3_Dehydroquinate + H+ + NADH		 (( (( (E * kcat_A) ) * A) ) / (Km_A + A)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant S22A]

NAD+ + Quinate -> NADH + H+ + 3_Dehydroquinate		 (( (( (E * kcat_A) ) * A) ) / (Km_A + A)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant S22A]

NAD+ + Shikimate -> 3_Dehydroshikimate + NADH + H+		 (( (( (E * kcat_B) ) * B) ) / (Km_B + B)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) wildtype]

NAD+ + Shikimate -> 3_Dehydroshikimate + H+ + NADH		 (( (( (E * kcat_A) ) * A) ) / (Km_A + A)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant Y39F]

Shikimate + NAD+ -> 3_Dehydroshikimate + NADH + H+		 (( (( (E * kcat_A) ) * A) ) / (Km_A + A)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant S67A]

NAD+ + Shikimate -> NADH + H+ + 3_Dehydroshikimate		 (( (( (E * kcat_A) ) * A) ) / (Km_A + A)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant Y39F]

NAD+ + Quinate -> H+ + NADH + 3_Dehydroquinate		 (( (( (E * kcat_A) ) * A) ) / (Km_A + A)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant S67A]

NAD+ + Quinate -> 3_Dehydroquinate + NADH + H+		 (( (( (E * kcat_A) ) * A) ) / (Km_A + A)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant Q262A]

Quinate + NAD+ -> 3_Dehydroquinate + NADH + H+		 (( (( (E * kcat_A) ) * A) ) / (Km_A + A)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant T106A]

Quinate + NAD+ -> NADH + H+ + 3_Dehydroquinate		 (( (( (E * kcat_A) ) * A) ) / (Km_A + A)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant K71A]

Quinate + NAD+ -> NADH + H+ + 3_Dehydroquinate		 (( (( (E * kcat_A) ) * A) ) / (Km_A + A)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant T106A]

NAD+ + Shikimate -> 3_Dehydroshikimate + H+ + NADH		 (( (( (E * kcat_A) ) * A) ) / (Km_A + A)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant D107A]

NAD+ + Shikimate -> NADH + 3_Dehydroshikimate + H+		 (( (( (E * kcat_A) ) * A) ) / (Km_A + A)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant Q262A]

Shikimate + NAD+ -> 3_Dehydroshikimate + NADH + H+		 (( (( (E * kcat_A) ) * A) ) / (Km_A + A)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant K71A]

Shikimate + NAD+ -> 3_Dehydroshikimate + NADH + H+		 (( (( (E * kcat_A) ) * A) ) / (Km_A + A)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) wildtype]

Quinate + NAD+ -> NADH + H+ + 3_Dehydroquinate		 (( (( (E * kcat_B) ) * B) ) / (Km_B + B)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant S22A]

NAD+ + Quinate -> NADH + 3_Dehydroquinate + H+		 (( (( (E * kcat_B) ) * B) ) / (Km_B + B)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant Y39F]

Quinate + NAD+ -> H+ + 3_Dehydroquinate + NADH		 (( (( (E * kcat_B) ) * B) ) / (Km_B + B)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant S67A]

Quinate + NAD+ -> NADH + 3_Dehydroquinate + H+		 (( (( (E * kcat_B) ) * B) ) / (Km_B + B)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant K71A]

NAD+ + Quinate -> NADH + H+ + 3_Dehydroquinate		 (( (( (E * kcat_B) ) * B) ) / (Km_B + B)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant S22A]

NAD+ + Shikimate -> 3_Dehydroshikimate + NADH + H+		 (( (( (E * kcat_B) ) * B) ) / (Km_B + B)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) wildtype]

NAD+ + Shikimate -> 3_Dehydroshikimate + NADH + H+		 (( (( (E * kcat_B) ) * B) ) / (Km_B + B)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant D107A]

NAD+ + Shikimate -> 3_Dehydroshikimate + H+ + NADH		 (( (( (E * kcat_B) ) * B) ) / (Km_B + B)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant K71A]

NAD+ + Shikimate -> H+ + NADH + 3_Dehydroshikimate		 (( (( (E * kcat_B) ) * B) ) / (Km_B + B)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant S67A]

NAD+ + Shikimate -> H+ + NADH + 3_Dehydroshikimate		 (( (( (E * kcat_B) ) * B) ) / (Km_B + B)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant T106A]

NAD+ + Shikimate -> NADH + H+ + 3_Dehydroshikimate		 (( (( (E * kcat_B) ) * B) ) / (Km_B + B)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant Y39F]

Shikimate + NAD+ -> NADH + 3_Dehydroshikimate + H+		 (( (( (E * kcat_B) ) * B) ) / (Km_B + B)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant Q262A]

NAD+ + Quinate -> H+ + NADH + 3_Dehydroquinate		 (( (( (E * kcat_B) ) * B) ) / (Km_B + B)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant T106A]

Quinate + NAD+ -> 3_Dehydroquinate + H+ + NADH		 (( (( (E * kcat_B) ) * B) ) / (Km_B + B)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) mutant Q262A]

Shikimate + NAD+ -> H+ + NADH + 3_Dehydroshikimate		 (( (( (E * kcat_B) ) * B) ) / (Km_B + B)) buffer: 100 mM Tris/HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) wildtype YdiB]

NADP+ + Shikimate -> H+ + NADPH + 3_Dehydroshikimate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) wildtype YdiB]

Shikimate + NADP+ -> H+ + NADPH + 3_Dehydroshikimate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) wildtype YdiB]

NAD+ + Shikimate -> 3_Dehydroshikimate + H+ + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) wildtype YdiB]

Shikimate + NAD+ -> H+ + 3_Dehydroshikimate + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) wildtype YdiB]

NAD+ + Quinate -> 3_Dehydroquinate + NADH + H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) wildtype YdiB]

NAD+ + Quinate -> 3_Dehydroquinate + NADH + H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) wildtype YdiB]

Quinate + NADP+ -> NADPH + 3_Dehydroquinate + H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 9
Temperature: 20
uniprot:P0A6D5 [quinate/shikimate dehydrogenase(Enzyme) wildtype YdiB]

NADP+ + Quinate -> 3_Dehydroquinate + NADPH + H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 9
Temperature: 20

Pathways

pathway id name
BioCyc:META_PWY-6628 superpathway of L-phenylalanine biosynthesis
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:ECOO157_PWY-6630 superpathway of tyrosine biosynthesis
BioCyc:ECOL199310_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:ECOL199310_ARO-PWY chorismate biosynthesis I
BioCyc:ECOL199310_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:ECOL199310_PWY-6628 superpathway of phenylalanine biosynthesis
BioCyc:ECOL316407_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:META_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:ECO_ARO-PWY chorismate biosynthesis I
BioCyc:ECOO157_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:ECOO157_PWY-6629 superpathway of tryptophan biosynthesis
BioCyc:ECOL316407_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:ECOL316407_ARO-PWY chorismate biosynthesis I
BioCyc:META_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:META_ARO-PWY chorismate biosynthesis I
BioCyc:ECO_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:ECOO157_ARO-PWY chorismate biosynthesis I
BioCyc:ECOO157_PWY-6628 superpathway of phenylalanine biosynthesis
BioCyc:ECOL199310_PWY-6629 superpathway of tryptophan biosynthesis
BioCyc:META_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:META_PWY-6629 superpathway of L-tryptophan biosynthesis
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECOO157_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
BioCyc:ECOL199310_PWY-6630 superpathway of tyrosine biosynthesis
BioCyc:ECOL199310_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECOL316407_PWY-6163 chorismate biosynthesis from 3-dehydroquinate
PathBank:SMP0000836 Chorismate Biosynthesis
PathBank:SMP0001001 Secondary Metabolites: Shikimate Pathway