EC: 1.3.3.4

protoporphyrinogen oxidase (protoporphyrinogen-IX:oxygen oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P32397 [protoporphyrinogen oxidase(Enzyme) wildtype]

Protoporphyrinogen_IX + 3 O2 -> 3 H2O2 + Protoporphyrin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM sodium phosphate, 0.1 mM EDTA, 0.1% Tween 20, 5 µM FAD, 2% DMSO, 500 mM imidazole
PH: 7.4
Temperature: 25
uniprot:P32397 [protoporphyrinogen oxidase(Enzyme) mutant Val311Met]

3 O2 + Protoporphyrinogen_IX -> 3 H2O2 + Protoporphyrin		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Sodium phosphate, 0.1 mM EDTA, 0.1% Tween 20, 5 µM FAD, 2% DMSO, 500 mM imidazole
PH: 7.4
Temperature: 25
uniprot:P32397 [FAD]

3 O2 + Coproporphyrinogen_III -> Coproporphyrin_III + 3 H2O2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Trhis-HCl, 3 mM DTT, 1 mM EDTA, 0.1% Tween 20
PH: 8.7
Temperature: 36
uniprot:P32397 [FAD]

3 O2 + Protoporphyrinogen_IX -> Protoporphyrin + 3 H2O2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Trhis-HCl, 3 mM DTT, 1 mM EDTA, 0.1% Tween 20
PH: 8.7
Temperature: 36
uniprot:P32397 [FAD]

3 O2 + Mesoporphyrinogen_IX -> Mesoporphyrin_IX + 3 H2O2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Trhis-HCl, 3 mM DTT, 1 mM EDTA, 0.1% Tween 20
PH: 8.7
Temperature: 36
uniprot:D3ZVN7 [protoporphyrinogen oxidase(Enzyme) wildtype]

2 Protoporphyrinogen_IX + 3 O2 -> 6 H2O + 2 Protoporphyrin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.25 M Tris/HCl, 5 mM EDTA, 0.2 M Ascorbic acid, 0.5% (v/v) Triton X-100
PH: 8.5
Temperature: 37
uniprot:P32397 [protoporphyrinogen oxidase(Enzyme) wildtype]

Protoporphyrinogen_IX + 3 O2 -> 3 H2O2 + Protoporphyrin		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Potassium phosphate, 2.5 mM DTT, 1 mM EDTA, 0.2 M Imidazole, 0.03% Tween 80 (v/v)
PH: 7.5
Temperature: 25
uniprot:P50336 [protoporphyrinogen oxidase(Enzyme) wildtype]

3 O2 + Protoporphyrinogen_IX -> Protoporphyrin + 3 H2O2		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM NaH2PO4, 0.2% Tween 20 (v/v), 2.5 mM Glutathione
PH: 8
Temperature: 37
uniprot:P40012 [protoporphyrinogen oxidase(Enzyme) wildtype]

3 O2 + Protoporphyrinogen_IX -> 3 H2O2 + Protoporphyrin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 1 mM EDTA, 5 mM GSH
PH: 7.45
Temperature: 25
uniprot:P56601 [protoporphyrinogen oxidase(Enzyme) wildtype]

3 O2 + Protoporphyrinogen_IX -> 3 H2O2 + Protoporphyrin		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM NaH2PO4, 0.2% Tween 20 (v/v), 2.5 mM Glutathione
PH: 8
Temperature: 37
uniprot:O67814 [protoporphyrinogen oxidase(Enzyme) wildtype]

Protoporphyrinogen_IX + 3 O2 -> Protoporphyrin + 3 H2O2		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM NaH2PO4, 0.2% Tween 20 (v/v), 2.5 mM Glutathione
PH: 8
Temperature: 50
uniprot:P32397 [protoporphyrinogen oxidase(Enzyme) wildtype]

Protoporphyrinogen_IX + 3 O2 -> Protoporphyrin + 3 H2O2		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Potassium phosphate, 2.5 mM DTT, 1 mM EDTA, 0.2 M Imidazole, 0.03% Tween 80 (v/v)
PH: 7.5
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY-5920 superpathway of b heme biosynthesis from glycine
BioCyc:ARA_HEME-BIOSYNTHESIS-II heme biosynthesis I (aerobic)
BioCyc:ARA_CHLOROPHYLL-SYN 3,8-divinyl-chlorophyllide a biosynthesis I (aerobic, light-dependent)
BioCyc:HUMAN_PWY-5920 heme biosynthesis
BioCyc:META_CHLOROPHYLL-SYN 3,8-divinyl-chlorophyllide a biosynthesis I (aerobic, light-dependent)
BioCyc:MOUSE_HEME-BIOSYNTHESIS-II heme biosynthesis from uroporphyrinogen-III I
BioCyc:HUMAN_HEME-BIOSYNTHESIS-II heme biosynthesis from uroporphyrinogen-III I
BioCyc:META_HEME-BIOSYNTHESIS-II heme b biosynthesis I (aerobic)
BioCyc:YEAST_HEME-BIOSYNTHESIS-II heme biosynthesis
BioCyc:YEAST_PWY-5920 superpathway of heme biosynthesis
BioCyc:ARA_PWYQT-62 superpathway of proto- and siroheme biosynthesis
BioCyc:META_PWY-5918 superpathay of heme b biosynthesis from glutamate
BioCyc:MOUSE_PWY-5920 heme biosynthesis II
PlantCyc:NTABACUM_TN90_CHLOROPHYLL-SYN 3,8-divinyl-chlorophyllide a biosynthesis I (aerobic, light-dependent)
PlantCyc:NTABACUM_TN90_PWY-5918 superpathway of heme b biosynthesis from glutamate
PlantCyc:PLANT_PWY-5918 superpathay of heme b biosynthesis from glutamate
PlantCyc:ARA_PWYQT-62 superpathway of proto- and siroheme biosynthesis
PlantCyc:ARA_HEME-BIOSYNTHESIS-II heme b biosynthesis I (aerobic)
PlantCyc:PLANT_HEME-BIOSYNTHESIS-II heme b biosynthesis I (aerobic)
PlantCyc:PLANT_CHLOROPHYLL-SYN 3,8-divinyl-chlorophyllide a biosynthesis I (aerobic, light-dependent)
PlantCyc:ARA_CHLOROPHYLL-SYN 3,8-divinyl-chlorophyllide a biosynthesis I (aerobic, light-dependent)
PlantCyc:ARA_PWY-5918 superpathay of heme b biosynthesis from glutamate
PlantCyc:NTABACUM_TN90_HEME-BIOSYNTHESIS-II heme b biosynthesis I (aerobic)
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-189445 Porphyrin metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SCE-189445 Porphyrin metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-DDI-189451 Heme biosynthesis
Reactome:R-SCE-1430728 Metabolism
Reactome:R-BTA-189451 Heme biosynthesis
Reactome:R-HSA-189445 Porphyrin metabolism
Reactome:R-MMU-189445 Porphyrin metabolism
Reactome:R-SCE-189451 Heme biosynthesis
Reactome:R-SPO-189445 Porphyrin metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-189445 Porphyrin metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-189451 Heme biosynthesis
Reactome:R-MMU-189451 Heme biosynthesis
Reactome:R-SPO-189451 Heme biosynthesis
PathBank:SMP0000024 Porphyrin Metabolism
PathBank:SMP0000345 Congenital Erythropoietic Porphyria (CEP) or Gunther Disease
PathBank:SMP0000344 Acute Intermittent Porphyria
PathBank:SMP0002363 Porphyrin Metabolism
PathBank:SMP0000346 Porphyria Variegata (PV)
PathBank:SMP0000342 Hereditary Coproporphyria (HCP)