EC: 1.3.1.12

prephenate dehydrogenase (prephenate:NAD+ oxidoreductase (decarboxylating))

enzyme lambda metabolic reaction experiment
uniprot:P07023 [prephenate dehydrogenase(Enzyme) wildtype]

NAD+ + Prephenate -> NADH + 3_(4_Hydroxyphenyl)pyruvate + CO2 + H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) mutant H131A]

Prephenate + NAD+ -> H+ + 3_(4_Hydroxyphenyl)pyruvate + NADH + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) mutant H347N]

NAD+ + Prephenate -> 3_(4_Hydroxyphenyl)pyruvate + CO2 + NADH + H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) mutant H197N]

NAD+ + Prephenate -> NADH + CO2 + H+ + 3_(4_Hydroxyphenyl)pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) mutant H257A]

Prephenate + NAD+ -> NADH + 3_(4_Hydroxyphenyl)pyruvate + CO2 + H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) mutant H153N]

Prephenate + NAD+ -> H+ + CO2 + 3_(4_Hydroxyphenyl)pyruvate + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) mutant H239N]

Prephenate + NAD+ -> H+ + 3_(4_Hydroxyphenyl)pyruvate + NADH + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) mutant H245N]

NAD+ + Prephenate -> NADH + 3_(4_Hydroxyphenyl)pyruvate + CO2 + H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) mutant K37Q]

NAD+ + Prephenate -> 3_(4_Hydroxyphenyl)pyruvate + CO2 + NADH + H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) mutant K37Q]

NAD+ + Prephenate -> 3_(4_Hydroxyphenyl)pyruvate + CO2 + NADH + H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) mutant H131A]

NAD+ + Prephenate -> NADH + H+ + CO2 + 3_(4_Hydroxyphenyl)pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) mutant H153N]

Prephenate + NAD+ -> 3_(4_Hydroxyphenyl)pyruvate + NADH + CO2 + H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) mutant H239N]

Prephenate + NAD+ -> H+ + CO2 + NADH + 3_(4_Hydroxyphenyl)pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) wildtype]

Prephenate + NAD+ -> NADH + 3_(4_Hydroxyphenyl)pyruvate + CO2 + H+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) mutant H257A]

NAD+ + Prephenate -> H+ + 3_(4_Hydroxyphenyl)pyruvate + CO2 + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) mutant H265A]

Prephenate + NAD+ -> H+ + NADH + 3_(4_Hydroxyphenyl)pyruvate + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) mutant H245N]

Prephenate + NAD+ -> 3_(4_Hydroxyphenyl)pyruvate + H+ + CO2 + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) mutant H197N]

NAD+ + Prephenate -> CO2 + H+ + 3_(4_Hydroxyphenyl)pyruvate + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) wildtype]

NAD+ + Prephenate -> CO2 + H+ + NADH + 3_(4_Hydroxyphenyl)pyruvate		 (( (( (( (E * kcat0) ) / (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKa2))))) ) * A) ) / (( (( (kcat0 / (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKa2))))) ) / (kcatKm0 / (( (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKa1)))) ) + (( (10 ^ (0 - pKb1)) ) / (10 ^ (0 - pH)))))) ) + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 5.5
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) mutant H265A]

NAD+ + Prephenate -> CO2 + H+ + 3_(4_Hydroxyphenyl)pyruvate + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30
uniprot:P07023 [prephenate dehydrogenase(Enzyme) mutant H347N]

NAD+ + Prephenate -> 3_(4_Hydroxyphenyl)pyruvate + CO2 + H+ + NADH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.10 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM EDTA, 1 mM DTT
PH: 7.2
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-6628 superpathway of L-phenylalanine biosynthesis
BioCyc:META_PHESYN L-phenylalanine biosynthesis I
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:ECOL316407_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:META_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:META_PWY-7303 3-dimethylallyl-4-hydroxybenzoate biosynthesis
BioCyc:MTBH37RV_TYRSYN L-tyrosine biosynthesis I
BioCyc:ECOL316407_COMPLETE-ARO-PWY superpathway of phenylalanine, tyrosine, and tryptophan biosynthesis
BioCyc:GCF_000013425_TYRSYN L-tyrosine biosynthesis I
BioCyc:META_TYRSYN L-tyrosine biosynthesis I
BioCyc:META_COMPLETE-ARO-PWY superpathway of aromatic amino acid biosynthesis
BioCyc:BSUB_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:BSUB_TYRSYN L-tyrosine biosynthesis I
BioCyc:GCF_000013425_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:META_PWY-7287 novobiocin biosynthesis
BioCyc:ECO_TYRSYN L-tyrosine biosynthesis I
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_PHESYN L-phenylalanine biosynthesis I
BioCyc:MTBH37RV_PWY-6630 superpathway of L-tyrosine biosynthesis
BioCyc:ECOL316407_TYRSYN tyrosine biosynthesis I
BioCyc:ECOL316407_PHESYN phenylalanine biosynthesis I
PathBank:SMP0000826 Tyrosine Biosynthesis