EC: 1.14.14.151

premnaspirodiene oxygenase ((–)-vetispiradiene,[reduced NADPH-hemoprotein reductase]:oxygen 2alpha-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:Q94FM7 [premnaspirodiene oxygenase(Enzyme) wildtype]

NADPH + Vetispiradiene + O2 -> H+ + H2O + NADP+ + Solavetivol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:Q94FM7 [premnaspirodiene oxygenase(Enzyme) mutant S368V/I484V]

O2 + NADPH + Vetispiradiene -> H2O + Solavetivol + NADP+ + H+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:Q94FM7 [premnaspirodiene oxygenase(Enzyme) mutant S368V]

O2 + NADPH + Vetispiradiene -> NADP+ + H2O + H+ + Solavetivol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:A6YIH8 [premnaspirodiene oxygenase(Enzyme) wildtype]

O2 + NADPH + 4_epi_Eremophilene -> 2_beta_Hydroxy_4_epi_eremophilene + NADP+ + H2O + H+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:A6YIH8 [premnaspirodiene oxygenase(Enzyme) wildtype]

NADPH + O2 + Valencene -> H+ + beta_Nootkatol + NADP+ + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:A6YIH8 [premnaspirodiene oxygenase(Enzyme) wildtype]

NADPH + Valencene + O2 -> H2O + alpha_Nootkatol + H+ + NADP+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:A6YIH8 [premnaspirodiene oxygenase(Enzyme) wildtype]

Solavetivol + O2 + NADPH -> H+ + Solavetivone + H2O + NADP+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:A6YIH8 [premnaspirodiene oxygenase(Enzyme) wildtype]

O2 + 5_Epiaristolochene + NADPH -> 2_beta_Hydroxy_5_epiaristolochene + H2O + NADP+ + H+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:A6YIH8 [premnaspirodiene oxygenase(Enzyme) wildtype]

alpha_Cedrene + O2 + NADPH -> NADP+ + H+ + H2O + Cedr_8_ene_15_ol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:A6YIH8 [premnaspirodiene oxygenase(Enzyme) wildtype]

NADPH + O2 + Vetispiradiene -> H+ + Solavetivol + H2O + NADP+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:A6YIH8 [premnaspirodiene oxygenase(Enzyme) wildtype]

Vetispiradiene + 2 NADPH + 2 O2 -> 2 H+ + 2 NADP+ + Solavetivone + 2 H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:A6YIH8 [premnaspirodiene oxygenase(Enzyme) mutant V480I/A484I]

Vetispiradiene + NADPH + O2 -> NADP+ + H2O + H+ + Solavetivol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:A6YIH8 [premnaspirodiene oxygenase(Enzyme) mutant V482I/A484I]

Vetispiradiene + O2 + NADPH -> Solavetivol + H2O + NADP+ + H+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:A6YIH8 [premnaspirodiene oxygenase(Enzyme) mutant V482I]

O2 + Vetispiradiene + NADPH -> H+ + H2O + Solavetivol + NADP+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:Q94FM7 [premnaspirodiene oxygenase(Enzyme) mutant S368V/S482V]

O2 + NADPH + Vetispiradiene -> Solavetivol + H+ + H2O + NADP+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:A6YIH8 [premnaspirodiene oxygenase(Enzyme) mutant V480I/A484I]

NADPH + O2 + Valencene -> NADP+ + beta_Nootkatol + H2O + H+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:A6YIH8 [premnaspirodiene oxygenase(Enzyme) mutant V482I/A484I]

O2 + NADPH + Valencene -> H+ + NADP+ + beta_Nootkatol + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:A6YIH8 [premnaspirodiene oxygenase(Enzyme) mutant V482I]

O2 + NADPH + Valencene -> NADP+ + beta_Nootkatol + H2O + H+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:A6YIH8 [premnaspirodiene oxygenase(Enzyme) mutant V480I/A484I]

O2 + 5_Epiaristolochene + NADPH -> H+ + H2O + NADP+ + 2_beta_Hydroxy_5_epiaristolochene		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:A6YIH8 [premnaspirodiene oxygenase(Enzyme) mutant V482I]

NADPH + 5_Epiaristolochene + O2 -> 2_beta_Hydroxy_5_epiaristolochene + H+ + H2O + NADP+		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30
uniprot:A6YIH8 [premnaspirodiene oxygenase(Enzyme) mutant V482I/A484I]

NADPH + 5_Epiaristolochene + O2 -> H2O + H+ + NADP+ + 2_beta_Hydroxy_5_epiaristolochene		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 4% (v/v) Me2SO
PH: 7.5
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-2961 sesquiterpenoid phytoalexins biosynthesis
PlantCyc:PLANT_PWY-2961 sesquiterpenoid phytoalexins biosynthesis