Isoleucine Biosynthesis

Metabolites

(3R)-3-Methyl-2-oxopentanoic acid

Formula: C6H10O3 (130.062991)

CAS ID: 61748-89-8

(R)-2,3-Dihydroxy-3-methylpentanoate

Formula: C6H12O4 (148.0735552)

CAS ID: 562-43-6

(S)-2-Aceto-2-hydroxybutanoate

Formula: C6H10O4 (146.057906)

CAS ID: 113919-07-6

2-Oxobutanoate

Formula: C4H6O3 (102.0316926)

CAS ID: 600-18-0

2-Oxoglutarate

Formula: C5H6O5 (146.0215226)

CAS ID: 328-50-7

ADP

Formula: C10H15N5O10P2 (427.02941500000003)

CAS ID: 58-64-0

ATP

Formula: C10H16N5O13P3 (506.9957476)

CAS ID: 56-65-5

CO2

Formula: CO2 (43.98983)

CAS ID: 124-38-9

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

L-Glutamate

Formula: C5H9NO4 (147.0531554)

CAS ID: 56-86-0

L-Isoleucine

Formula: C6H13NO2 (131.0946238)

CAS ID: 73-32-5

L-Threonine

Formula: C4H9NO3 (119.0582404)

CAS ID: 72-19-5

NADP+

Formula: C21H29N7O17P3 (744.0832754)

CAS ID: 53-59-8

NH4+

Formula: NH4 (18.0343724)

CAS ID: 14798-03-9

Pyruvate

Formula: C3H4O3 (88.0160434)

CAS ID: 127-17-3

Sodium cation

Formula: Na (22.98977)

CAS ID: 17341-25-2

phosphate(3-)

Formula: O4P (94.953423)

CAS ID: 14265-44-2



Enzyme

EC Number name full name note
1.1.1.86 ketol-acid reductoisomerase (NADP+) (R)-2,3-dihydroxy-3-methylbutanoate:NADP+ oxidoreductase (isomerizing)
2.2.1.6 acetolactate synthase pyruvate:pyruvate acetaldehydetransferase (decarboxylating)
2.6.1.42 branched-chain-amino-acid transaminase branched-chain-amino-acid:2-oxoglutarate aminotransferase
4.2.1.9 dihydroxy-acid dehydratase 2,3-dihydroxy-3-methylbutanoate hydro-lyase (3-methyl-2-oxobutanoate-forming)
4.3.1.19 threonine ammonia-lyase L-threonine ammonia-lyase (2-oxobutanoate-forming)


Pathway Source

Organism Taxonomy Group Enzyme List Source Ratio
Lachancea fermentati MAGs 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Acer saccharum MAGs 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Citrus sinensis plant 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Streptomyces halstedii bacteria 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Streptomyces albogriseolus bacteria 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Aspergillus flavus fungi 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Histoplasma capsulatum fungi 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Aspergillus tubingensis fungi 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Oryza nivara MAGs 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Synechocystis sp. PCC 6803 bacteria 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Penicillium brevicompactum fungi 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Pseudomonas syringae pv. syringae MAGs 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Triticum aestivum plant 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Aspergillus niger fungi 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Aspergillus oryzae fungi 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Aspergillus parasiticus MAGs 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Phytophthora citrophthora MAGs 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Torulaspora delbrueckii fungi 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Zygosaccharomyces rouxii fungi 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%
Penicillium chrysogenum fungi 1.1.1.86 / 2.2.1.6 / 2.6.1.42 / 4.2.1.9 / 4.3.1.19 100.00%