Leucine Biosynthesis

Metabolites

(2R,3S)-3-Isopropylmalate

Formula: C7H12O5 (176.06847019999998)

CAS ID: 126576-14-5

(2S)-2-Isopropyl-3-oxosuccinate

Formula: C7H10O5 (174.052821)

CAS ID: 1245945-28-1

(3R)-3-Methyl-2-oxopentanoic acid

Formula: C6H10O3 (130.062991)

CAS ID: 61748-89-8

2-Isopropylmaleate

Formula: C7H10O4 (158.057906)

CAS ID: 44976-69-4

2-Oxoglutarate

Formula: C5H6O5 (146.0215226)

CAS ID: 328-50-7

4-Methyl-2-oxopentanoate

Formula: C6H10O3 (130.062991)

CAS ID: 816-66-0

Acetyl-CoA

Formula: C23H38N7O17P3S (809.1257688000001)

CAS ID: 72-89-9

alpha-Isopropylmalate

Formula: C7H12O5 (176.06847019999998)

CAS ID: 49601-06-1

CO2

Formula: CO2 (43.98983)

CAS ID: 124-38-9

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

L-Glutamate

Formula: C5H9NO4 (147.0531554)

CAS ID: 56-86-0

L-Leucine

Formula: C6H13NO2 (131.0946238)

CAS ID: 61-90-5

NAD+

Formula: C21H28N7O14P2 (664.1169428000001)

CAS ID: 53-84-9

NADH

Formula: C21H29N7O14P2 (665.1247674)

CAS ID: 58-68-4



Enzyme

EC Number name full name note
1.1.1.83 D-malate dehydrogenase (decarboxylating) (R)-malate:NAD+ oxidoreductase (decarboxylating)
1.1.1.85 3-isopropylmalate dehydrogenase (2R,3S)-3-isopropylmalate:NAD+ oxidoreductase
2.3.3.13 2-isopropylmalate synthase acetyl-CoA:3-methyl-2-oxobutanoate C-acetyltransferase (thioester-hydrolysing, carboxymethyl-forming)
2.6.1.107 beta-methylphenylalanine transaminase (2S,3S)-3-methylphenylalanine:2-oxoglutarate aminotransferase
2.6.1.42 branched-chain-amino-acid transaminase branched-chain-amino-acid:2-oxoglutarate aminotransferase
2.6.1.57 aromatic-amino-acid transaminase aromatic-amino-acid:2-oxoglutarate aminotransferase
4.2.1.33 3-isopropylmalate dehydratase (2R,3S)-3-isopropylmalate hydro-lyase (2-isopropylmaleate-forming)


Pathway Source

Organism Taxonomy Group Enzyme List Source Ratio
Escherichia coli K-12 MAGs 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.107 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 100.00%
Aspergillus terreus fungi 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%
Penicillium chrysogenum fungi 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%
Penicillium digitatum fungi 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%
Exophiala dermatitidis fungi 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%
Penicillium vulpinum fungi 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%
Trichophyton rubrum fungi 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%
Aspergillus niger fungi 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%
Aspergillus parasiticus MAGs 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%
Penicillium canescens fungi 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%
Penicillium griseofulvum fungi 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%
Monascus purpureus MAGs 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%
Penicillium brevicompactum fungi 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%
Penicillium citrinum fungi 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%
Trichophyton violaceum MAGs 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%
Penicillium crustosum fungi 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%
Aspergillus avenaceus MAGs 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%
Aspergillus tubingensis fungi 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%
Aspergillus flavus fungi 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%
Penicillium expansum fungi 1.1.1.83 / 1.1.1.85 / 2.3.3.13 / 2.6.1.42 / 2.6.1.57 / 4.2.1.33 85.71%