EC: 6.3.5.7

glutaminyl-tRNA synthase (glutamine-hydrolysing) (L-glutamyl-tRNAGln:L-glutamine amido-ligase (ADP-forming))

enzyme lambda metabolic reaction experiment
- [beta,gamma-Methylene ATP]

L_Glutamine + L_Glutamyl_tRNA(Gln) + ATP -> L_Glutamate + Phosphate + ADP + L_Glutaminyl_tRNA(Gln)		 (( (kcat * E) ) / (1 + (I / IC50))) buffer: 50 mM Hepes, 25 mM KCl, 15 mM MgCl2
PH: 7
Temperature: 22
- [ATP]

H2O + L_Glutamine -> NH3 + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 25 mM KCl, 15 mM MgCl2
PH: 7
Temperature: 22
- [glutaminyl-tRNA synthase (glutamine-hydrolysing)(Enzyme) wildtype]

L_Glutamine + ATP + L_Glutamyl_tRNA(Gln) -> ADP + L_Glutamate + L_Glutaminyl_tRNA(Gln) + Phosphate		 (( (( (E * kcat) ) * C) ) / (Km + C)) buffer: 50 mM Hepes, 25 mM KCl, 15 mM MgCl2
PH: 7
Temperature: 22
- [L-Glutamyl-tRNA(Gln)]

H2O + L_Glutamine -> NH3 + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 25 mM KCl, 15 mM MgCl2
PH: 7
Temperature: 22
- [glutaminyl-tRNA synthase (glutamine-hydrolysing)(Enzyme) wildtype]

L_Glutamine + ATP + L_Glutamyl_tRNA(Gln) -> ADP + L_Glutamate + Phosphate + L_Glutaminyl_tRNA(Gln)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 25 mM KCl, 15 mM MgCl2
PH: 7
Temperature: 22
- [glutaminyl-tRNA synthase (glutamine-hydrolysing)(Enzyme) wildtype]

L_Glutamine + L_Glutamyl_tRNA(Gln) + ATP -> L_Glutaminyl_tRNA(Gln) + Phosphate + L_Glutamate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 25 mM KCl, 15 mM MgCl2
PH: 7
Temperature: 22
- [glutaminyl-tRNA synthase (glutamine-hydrolysing)(Enzyme) wildtype]

L_Glutamine + L_Glutamyl_tRNA(Gln) + ATP -> L_Glutaminyl_tRNA(Gln) + ADP + L_Glutamate + Phosphate		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 50 mM Hepes, 25 mM KCl, 15 mM MgCl2
PH: 7
Temperature: 22
- [glutaminyl-tRNA synthase (glutamine-hydrolysing)(Enzyme) wildtype]

L_Glutamine + H2O -> L_Glutamate + NH3		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 25 mM KCl, 15 mM MgCl2
PH: 7
Temperature: 22
- [Adenosine 5-O-(3-thiotriphosphate)]

L_Glutamine + ATP + L_Glutamyl_tRNA(Gln) -> L_Glutamate + L_Glutaminyl_tRNA(Gln) + ADP + Phosphate		 (( (kcat * E) ) / (1 + (I / IC50))) buffer: 50 mM Hepes, 25 mM KCl, 15 mM MgCl2
PH: 7
Temperature: 22
- [Trinitrophenyl-ATP]

L_Glutamine + L_Glutamyl_tRNA(Gln) + ATP -> L_Glutaminyl_tRNA(Gln) + Phosphate + L_Glutamate + ADP		 (( (kcat * E) ) / (1 + (I / IC50))) buffer: 50 mM Hepes, 25 mM KCl, 15 mM MgCl2
PH: 7
Temperature: 22
- [glutaminyl-tRNA synthase (glutamine-hydrolysing)(Enzyme) wildtype]

L_Glutamyl_tRNA(Gln) + L_Glutamine + ATP -> L_Glutamate + Phosphate + L_Glutaminyl_tRNA(Gln) + ADP		 (( (kcat * E) ) / (1 + (I / IC50))) buffer: 50 mM Hepes, 25 mM KCl, 15 mM MgCl2
PH: 7
Temperature: 22

Pathways

pathway id name
BioCyc:META_PWY490-4 L-asparagine biosynthesis III (tRNA-dependent)
BioCyc:HPY_PWY-5921 L-glutamine biosynthesis II (tRNA-dependent)
BioCyc:CAULONA1000_PWY-5921 glutaminyl-tRNAgln biosynthesis via transamidation
BioCyc:CORYNE_PWY-5921 glutaminyl-tRNAgln biosynthesis via transamidation
BioCyc:HUMAN_PWY-5921 L-glutamine tRNA biosynthesis
BioCyc:META_PWY-5921 glutaminyl-tRNAgln biosynthesis via transamidation
BioCyc:ARA_PWY-5921 glutaminyl-tRNAgln biosynthesis via transamidation
BioCyc:MTBH37RV_PWY490-4 L-asparagine biosynthesis III (tRNA-dependent)
BioCyc:CAULO_PWY490-4 asparagine biosynthesis III (tRNA-dependent)
BioCyc:ANTHRA_PWY-5921 glutaminyl-tRNAgln biosynthesis via transamidation
BioCyc:SYNEL_PWY490-4 L-asparagine biosynthesis III (tRNA-dependent)
BioCyc:AGRO_PWY-5921 glutaminyl-tRNAgln biosynthesis via transamidation
BioCyc:MTBH37RV_PWY-5921 glutaminyl-tRNAgln biosynthesis via transamidation
BioCyc:MTBCDC1551_PWY-5921 glutaminyl-tRNAgln biosynthesis via transamidation
BioCyc:HPY_PWY490-4 asparagine biosynthesis III (tRNA-dependent)
BioCyc:CAULO_PWY-5921 L-glutamine biosynthesis II (tRNA-dependent)
BioCyc:MOUSE_TRNA-CHARGING-PWY tRNA charging pathway
BioCyc:BSUB_PWY-5921 glutaminyl-tRNAgln biosynthesis via transamidation
BioCyc:SYNEL_PWY-5921 glutaminyl-tRNAgln biosynthesis via transamidation
BioCyc:GCF_000013425_PWY-5921 glutaminyl-tRNAgln biosynthesis via transamidation