EC: 6.3.5.3

phosphoribosylformylglycinamidine synthase (N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide:L-glutamine amido-ligase (ADP-forming))

enzyme lambda metabolic reaction experiment
uniprot:P15254 [beta,gamma-Methylene ATP]

H2O + ATP + 5'_Phosphoribosyl_N_formylglycinamide + L_Glutamine -> ADP + 2_(Formamido)_N1_(5'_phosphoribosyl)acetamidine + Phosphate + L_Glutamate		 (( (Vmax * A) ) / (( (Kma * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) buffer: 40 mM HEPES, 20 mM MgCl2, 10 mM KCl
PH: 7.25
Temperature: 37
uniprot:P15254 [beta,gamma-Methylene ATP]

ATP + 5'_Phosphoribosyl_N_formylglycinamide + H2O + L_Glutamine -> ADP + 2_(Formamido)_N1_(5'_phosphoribosyl)acetamidine + Phosphate + L_Glutamate		 (( (Vmax * A) ) / (( (Kma * (1 + (I / Kis))) ) + A)) buffer: 40 mM HEPES, 20 mM MgCl2, 10 mM KCl
PH: 7.25
Temperature: 37
uniprot:P15254 [beta,gamma-Methylene ATP]

H2O + ATP + L_Glutamine + 5'_Phosphoribosyl_N_formylglycinamide -> ADP + Phosphate + L_Glutamate + 2_(Formamido)_N1_(5'_phosphoribosyl)acetamidine		 (( (Vmax * A) ) / (Kma + (A * (1 + (I / Kii))))) buffer: 40 mM HEPES, 20 mM MgCl2, 10 mM KCl
PH: 7.25
Temperature: 37
uniprot:P15254 [L-Albizziine]

ATP + H2O + 5'_Phosphoribosyl_N_formylglycinamide + L_Glutamine -> Phosphate + L_Glutamate + ADP + 2_(Formamido)_N1_(5'_phosphoribosyl)acetamidine		 (( (Vmax * A) ) / (( (Kma * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) buffer: 40 mM HEPES, 20 mM MgCl2, 10 mM KCl
PH: 7.25
Temperature: 37
uniprot:P15254 [L-Albizziine]

5'_Phosphoribosyl_N_formylglycinamide + H2O + ATP + L_Glutamine -> Phosphate + L_Glutamate + 2_(Formamido)_N1_(5'_phosphoribosyl)acetamidine + ADP		 (( (Vmax * A) ) / (( (Kma * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) buffer: 40 mM HEPES, 20 mM MgCl2, 10 mM KCl
PH: 7.25
Temperature: 37
uniprot:P15254 [L-Albizziine]

H2O + L_Glutamine + ATP + 5'_Phosphoribosyl_N_formylglycinamide -> L_Glutamate + 2_(Formamido)_N1_(5'_phosphoribosyl)acetamidine + Phosphate + ADP		 (( (Vmax * A) ) / (( (Kma * (1 + (I / Kis))) ) + A)) buffer: 40 mM HEPES, 20 mM MgCl2, 10 mM KCl
PH: 7.25
Temperature: 37
uniprot:P15254 [phosphoribosylformylglycinamidine synthase(Enzyme) wildtype]

L_Glutamine + ATP + 5'_Phosphoribosyl_N_formylglycinamide + H2O -> 2_(Formamido)_N1_(5'_phosphoribosyl)acetamidine + Phosphate + ADP + L_Glutamate		 (( (Vmax * A) ) / (( (Kma * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) buffer: 40 mM HEPES, 20 mM MgCl2, 10 mM KCl
PH: 7.25
Temperature: 37
uniprot:P15254 [phosphoribosylformylglycinamidine synthase(Enzyme) wildtype]

ATP + H2O + 5'_Phosphoribosyl_N_formylglycinamide + L_Glutamine -> Phosphate + 2_(Formamido)_N1_(5'_phosphoribosyl)acetamidine + ADP + L_Glutamate		 (( (Vmax * A) ) / (( (Kma * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) buffer: 40 mM HEPES, 20 mM MgCl2, 10 mM KCl
PH: 7.25
Temperature: 37
uniprot:P15254 [phosphoribosylformylglycinamidine synthase(Enzyme) wildtype]

5'_Phosphoribosyl_N_formylglycinamide + L_Glutamine + H2O + ATP -> L_Glutamate + ADP + 2_(Formamido)_N1_(5'_phosphoribosyl)acetamidine + Phosphate		 (( (Vmax * A) ) / (( (Kma * (1 + (I / Kis))) ) + A)) buffer: 40 mM HEPES, 20 mM MgCl2, 10 mM KCl
PH: 7.25
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:META_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:ECO_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:YEAST_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:ARA_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:MTBCDC1551_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:MTBCDC1551_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:ECOL199310_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:MOUSE_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:BSUB_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:ECOL316407_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:CORYNE_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:GCF_000013425_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:YEAST_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:VCHO_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:MTBH37RV_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:ECOO157_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:ECOL199310_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:CAULO_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:ANTHRA_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:ANTHRA_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:MOUSE_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:SYNEL_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:SYNEL_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:ECOL316407_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:CORYNE_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:ECO_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:ECO_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:ARA_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:VCHO_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:VCHO_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:ECOO157_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:CAULO_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:CAULO_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:ANTHRA_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:ANTHRA_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:AGRO_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:MOUSE_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:BSUB_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:ECOL316407_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:HUMAN_PWY-841 purine nucleotides de novo biosynthesis
BioCyc:META_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:META_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:META_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:VCHO_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:MTBH37RV_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:MTBH37RV_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:MTBCDC1551_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:MTBCDC1551_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:ECOO157_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:ECOL199310_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:ECOL199310_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:CAULO_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:ANTHRA_DENOVOPURINE2-PWY superpathway of purine nucleotides de novo biosynthesis II
BioCyc:BSUB_PWY-6277 superpathway of 5-aminoimidazole ribonucleotide biosynthesis
BioCyc:BSUB_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:SYNEL_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:SYNEL_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:SYNEL_DENOVOPURINE2-PWY superpathway of purine nucleotides de novo biosynthesis II
BioCyc:CORYNE_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
PathBank:SMP0000927 Purine Nucleotides De Novo Biosynthesis
PathBank:SMP0002042 Thiamin Diphosphate Biosynthesis
PathBank:SMP0002047 Purine Nucleotides De Novo Biosynthesis 2
PlantCyc:ARA_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
PlantCyc:ARA_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
PlantCyc:ARA_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
Reactome:R-DDI-8956320 Nucleotide biosynthesis
Reactome:R-HSA-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-15869 Nucleotide metabolism
Reactome:R-CEL-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-DDI-15869 Nucleotide metabolism
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-HSA-8956320 Nucleotide biosynthesis
Reactome:R-MMU-8956320 Nucleotide biosynthesis
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-CEL-15869 Nucleotide metabolism
Reactome:R-DDI-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-DME-15869 Nucleotide metabolism
Reactome:R-SCE-15869 Nucleotide metabolism
Reactome:R-SPO-8956320 Nucleotide biosynthesis
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-8956320 Nucleotide biosynthesis
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DME-8956320 Nucleotide biosynthesis
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-SCE-8956320 Nucleotide biosynthesis
Reactome:R-SPO-73817 Purine ribonucleoside monophosphate biosynthesis
WikiPathways:WP4224 Purine metabolism and related disorders
PathBank:SMP0120448 Adenosine Deaminase Deficiency
PathBank:SMP0120452 AICA-Ribosiduria
PathBank:SMP0120508 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0120532 Molybdenum Cofactor Deficiency
PathBank:SMP0120536 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0120577 Xanthinuria Type I
PathBank:SMP0120601 Mitochondrial DNA Depletion Syndrome
PathBank:SMP0000167 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000220 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0000512 Xanthinuria Type I
PathBank:SMP0000536 Mitochondrial DNA Depletion Syndrome-3
PathBank:SMP0063668 Purine Metabolism
PathBank:SMP0120600 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0000050 Purine Metabolism
PathBank:SMP0000144 Adenosine Deaminase Deficiency
PathBank:SMP0000203 Molybdenum Cofactor Deficiency
PathBank:SMP0000428 Mercaptopurine Action Pathway
PathBank:SMP0000535 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0120449 Adenylosuccinate Lyase Deficiency
PathBank:SMP0120489 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0120497 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0120578 Xanthinuria Type II
PathBank:SMP0120602 Myoadenylate Deaminase Deficiency
PathBank:SMP0000365 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0000210 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0000430 Thioguanine Action Pathway
PathBank:SMP0000513 Xanthinuria Type II
PathBank:SMP0000537 Myoadenylate Deaminase Deficiency
PathBank:SMP0000364 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0000168 AICA-Ribosiduria
PathBank:SMP0000427 Azathioprine Action Pathway