EC: 6.3.2.5

phosphopantothenate-cysteine ligase (CTP) ((R)-4'-phosphopantothenate:L-cysteine ligase)

enzyme lambda metabolic reaction experiment
- [phosphopantothenate---cysteine ligase(Enzyme) wildtype]

L_Cysteine + D_4'_Phosphopantothenate + CTP -> (R)_4'_Phosphopantothenoyl_L_cysteine + Diphosphate + CMP		 (( (( (E * kcat) ) * C) ) / (( (( (Kmc + (C * (( (( (1 + (Kma / A)) ) + (Kmb / B)) ) + (( (Kia * Kmb) ) / (A * B))))) ) + (( (( (Kia * Kmb) ) * R) ) / (( (Kir * A) ) * B))) ) + (( (Kma * R) ) / (Kir * A)))) buffer: 30 µl commercial pyrophosphate reagent (Sigma-Aldrich), 70 µl 50 mM Tris-HCl, 10 mM DTT,
PH: 7.6
Temperature: 37
- [phosphopantothenate---cysteine ligase(Enzyme) wildtype]

CTP + L_Cysteine + D_4'_Phosphopantothenate -> CMP + Diphosphate + (R)_4'_Phosphopantothenoyl_L_cysteine		 (( (( (E * kcat) ) * A) ) / (( (Kma * (( (( (1 + (( (Kia * Kmb) ) / (Kma * B))) ) + (( (( (Kia * Kmb) ) * R) ) / (( (Kma * Kir) ) * B))) ) + (R / Kir))) ) + (A * (( (1 + (Kmb / B)) ) + (Kmc / C))))) buffer: 30 µl commercial pyrophosphate reagent (Sigma-Aldrich), 70 µl 50 mM Tris-HCl, 10 mM DTT,
PH: 7.6
Temperature: 37
- [phosphopantothenate---cysteine ligase(Enzyme) wildtype]

CTP + L_Cysteine + D_4'_Phosphopantothenate -> (R)_4'_Phosphopantothenoyl_L_cysteine + Diphosphate + CMP		 (( (( (( (( (E * kcat) ) * A) ) * B) ) * C) ) / (( (( (( (( (( (Kia * Kmb) ) * C) ) + (( (Kmc * A) ) * B)) ) + (( (Kmb * A) ) * C)) ) + (( (Kma * B) ) * C)) ) + (( (A * B) ) * C))) buffer: 30 µl commercial pyrophosphate reagent (Sigma-Aldrich), 70 µl 50 mM Tris-HCl, 10 mM DTT,
PH: 7.6
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-7851 coenzyme A biosynthesis II (eukaryotic)
BioCyc:ECO_COA-PWY coenzyme A biosynthesis I (prokaryotic)
BioCyc:VCHO_COA-PWY coenzyme A biosynthesis I (prokaryotic)
BioCyc:BSUB_COA-PWY coenzyme A biosynthesis I (prokaryotic)
BioCyc:ECOL316407_COA-PWY coenzyme A biosynthesis
BioCyc:META_PANTOSYN-PWY superpathway of coenzyme A biosynthesis I (bacteria)
BioCyc:MTBCDC1551_PANTOSYN-PWY pantothenate and coenzyme A biosynthesis I
BioCyc:META_COA-PWY coenzyme A biosynthesis I (prokaryotic)
BioCyc:ECO_PANTOSYN-PWY superpathway of coenzyme A biosynthesis I (bacteria)
BioCyc:YEAST_COA-PWY coenzyme A biosynthesis
BioCyc:MTBCDC1551_COA-PWY coenzyme A biosynthesis
BioCyc:BSUB_PANTOSYN-PWY superpathway of coenzyme A biosynthesis I (bacteria)
BioCyc:ECOL316407_PANTOSYN-PWY pantothenate and coenzyme A biosynthesis I
PathBank:SMP0000848 Pantothenate and CoA Biosynthesis
Reactome:R-SCE-196783 Coenzyme A biosynthesis
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SCE-199220 Vitamin B5 (pantothenate) metabolism
Reactome:R-SCE-196854 Metabolism of vitamins and cofactors
PathBank:SMP0002307 beta-Alanine Metabolism
PathBank:SMP0002364 Pantothenate and CoA Biosynthesis