EC: 6.3.2.2

glutamate-cysteine ligase (L-glutamate:L-cysteine gamma-ligase (ADP-forming))

enzyme lambda metabolic reaction experiment
uniprot:P46309 [glutamate---cysteine ligase(Enzyme) wildtype AtGCLdelta1-85]

L_Glutamate + ATP + L_Cysteine -> Phosphate + ADP + gamma_L_Glutamyl_L_cysteine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM MOPSO, 150 mM NaCl, 20 mM MgCl2, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml of type III rabbit muscle pyruvate kinase, and 20 U/ml of type II rabbit muscle lactate dehydrogenase.
PH: 7
Temperature: 25
uniprot:P46309 [glutamate---cysteine ligase(Enzyme) wildtype AtGCLdelta1-85]

ATP + L_Glutamate + L_Cysteine -> gamma_L_Glutamyl_L_cysteine + ADP + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM MOPSO, 150 mM NaCl, 20 mM MgCl2, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml of type III rabbit muscle pyruvate kinase, and 20 U/ml of type II rabbit muscle lactate dehydrogenase.
PH: 7
Temperature: 25
uniprot:P46309 [glutamate---cysteine ligase(Enzyme) wildtype AtGCLdelta1-85]

ATP + L_Glutamate + L_Cysteine -> ADP + Phosphate + gamma_L_Glutamyl_L_cysteine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM MOPSO, 150 mM NaCl, 20 mM MgCl2, 2 mM sodium phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml of type III rabbit muscle pyruvate kinase, and 20 U/ml of type II rabbit muscle lactate dehydrogenase.
PH: 7
Temperature: 25
- [glutamate---cysteine ligase(Enzyme) wildtype]

L_Cysteine + L_Glutamate + ATP -> ADP + gamma_L_Glutamyl_L_cysteine + Phosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM Tris-HCl, 2 mM EDTA, 20 mM MgCl2, 20 µg/ml BSA
PH: 8.2
Temperature: 37
- [glutamate---cysteine ligase(Enzyme) wildtype]

L_alpha_Aminobutyrate + ATP + L_Glutamate -> Phosphate + ADP + L_gamma_Glutamyl_L_alpha_aminobutyrate		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM Tris-HCl, 2 mM EDTA, 20 mM MgCl2, 20 µg/ml BSA
PH: 8.2
Temperature: 37
- [glutamate---cysteine ligase(Enzyme) wildtype]

alpha_Aminoethylglutarate + L_alpha_Aminobutyrate + ATP -> ADP + alpha_Aminoethylglutaryl_L_alpha_aminobutyrate + Phosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM Tris-HCl, 2 mM EDTA, 20 mM MgCl2, 20 µg/ml BSA
PH: 8.2
Temperature: 37
- [glutamate---cysteine ligase(Enzyme) wildtype]

S_Methyl_L_cysteine + ATP + L_Glutamate -> L_gamma_Glutamyl_S_methyl_L_cysteine + ADP + Phosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM Tris-HCl, 2 mM EDTA, 20 mM MgCl2, 20 µg/ml BSA
PH: 8.2
Temperature: 37
- [glutamate---cysteine ligase(Enzyme) wildtype]

L_Cysteine + ATP + alpha_Aminoethylglutarate -> Phosphate + ADP + alpha_Aminoethylglutaryl_L_cysteine		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM Tris-HCl, 2 mM EDTA, 20 mM MgCl2, 20 µg/ml BSA
PH: 8.2
Temperature: 37
- [glutamate---cysteine ligase(Enzyme) wildtype]

ATP + S_Methyl_L_cysteine + alpha_Aminoethylglutarate -> ADP + alpha_Aminoethylglutaryl_S_methyl_L_cysteine + Phosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM Tris-HCl, 2 mM EDTA, 20 mM MgCl2, 20 µg/ml BSA
PH: 8.2
Temperature: 37

Pathways

pathway id name
BioCyc:ARA_GLUTATHIONESYN-PWY glutathione biosynthesis
BioCyc:ECOO157_PWY-4041 γ-glutamyl cycle
BioCyc:HUMAN_PWY-4041 γ-glutamyl cycle
BioCyc:HUMAN_GLUTATHIONESYN-PWY glutathione biosynthesis
BioCyc:META_PWY-4041 γ-glutamyl cycle
BioCyc:META_PWY-7255 ergothioneine biosynthesis I (bacteria)
BioCyc:YEAST_GLUTATHIONESYN-PWY glutathione biosynthesis
BioCyc:ARA_PWY-4041 γ-glutamyl cycle
BioCyc:MOUSE_PWY-4041 γ-glutamyl cycle
BioCyc:ECO_GLUTATHIONESYN-PWY glutathione biosynthesis
BioCyc:ARA_PWYQT-4470 γ-glutamyl cycle (plant pathway)
BioCyc:ECOO157_GLUTATHIONESYN-PWY glutathione biosynthesis
BioCyc:ECOL199310_GLUTATHIONESYN-PWY glutathione biosynthesis
BioCyc:MOUSE_GLUTATHIONESYN-PWY glutathione biosynthesis
BioCyc:META_GLUTATHIONESYN-PWY glutathione biosynthesis
BioCyc:YEAST_PWY-4041 γ-glutamyl cycle
BioCyc:YEAST_PWY3O-114 superpathway of glutathione metabolism (truncated γ-glutamyl cycle)
BioCyc:VCHO_GLUTATHIONESYN-PWY glutathione biosynthesis
BioCyc:MTBH37RV_GLUTATHIONESYN-PWY glutathione biosynthesis
BioCyc:MTBH37RV_PWY-7255 ergothioneine biosynthesis I (bacteria)
BioCyc:ECOL316407_GLUTATHIONESYN-PWY glutathione biosynthesis
PathBank:SMP0000853 Glutathione Metabolism
PathBank:SMP0001941 Glutathione Metabolism II
PathBank:SMP0002032 Glutathione Metabolism III
PlantCyc:ARA_PWYQT-4470 γ-glutamyl cycle (plant pathway)
PlantCyc:ARA_PWY-4041 γ-glutamyl cycle
PlantCyc:ARA_GLUTATHIONESYN-PWY glutathione biosynthesis
PlantCyc:PLANT_PWY-4041 γ-glutamyl cycle
PlantCyc:PLANT_PWYQT-4470 γ-glutamyl cycle (plant pathway)
PlantCyc:PLANT_GLUTATHIONESYN-PWY glutathione biosynthesis
Reactome:R-HSA-9818027 NFE2L2 regulating anti-oxidant/detoxification enzymes
Reactome:R-HSA-9755511 KEAP1-NFE2L2 pathway
Reactome:R-HSA-9711123 Cellular response to chemical stress
Reactome:R-HSA-9759194 Nuclear events mediated by NFE2L2
Reactome:R-CEL-211859 Biological oxidations
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-RNO-156590 Glutathione conjugation
Reactome:R-SPO-1430728 Metabolism
Reactome:R-CEL-174403 Glutathione synthesis and recycling
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-174403 Glutathione synthesis and recycling
Reactome:R-HSA-156580 Phase II - Conjugation of compounds
Reactome:R-HSA-2262752 Cellular responses to stress
Reactome:R-MMU-156580 Phase II - Conjugation of compounds
Reactome:R-RNO-211859 Biological oxidations
Reactome:R-SPO-211859 Biological oxidations
Reactome:R-SPO-174403 Glutathione synthesis and recycling
Reactome:R-CEL-156580 Phase II - Conjugation of compounds
Reactome:R-DME-156580 Phase II - Conjugation of compounds
Reactome:R-HSA-156590 Glutathione conjugation
Reactome:R-MMU-156590 Glutathione conjugation
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-174403 Glutathione synthesis and recycling
Reactome:R-SPO-156580 Phase II - Conjugation of compounds
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-156590 Glutathione conjugation
Reactome:R-DME-211859 Biological oxidations
Reactome:R-DME-156590 Glutathione conjugation
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-174403 Glutathione synthesis and recycling
Reactome:R-HSA-8953897 Cellular responses to stimuli
Reactome:R-MMU-174403 Glutathione synthesis and recycling
Reactome:R-RNO-156580 Phase II - Conjugation of compounds
Reactome:R-SPO-156590 Glutathione conjugation
WikiPathways:WP4518 Gamma-glutamyl cycle for the biosynthesis and degradation of glutathione, including diseases
PathBank:SMP0120654 Cystinosis, Ocular Nonnephropathic
PathBank:SMP0120666 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120714 Homocarnosinosis
PathBank:SMP0120850 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120480 gamma-Glutamyltransferase Deficiency
PathBank:SMP0120496 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0120565 5-Oxoprolinase Deficiency
PathBank:SMP0121300 Glutathione Metabolism
PathBank:SMP0000015 Glutathione Metabolism
PathBank:SMP0000339 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0000500 5-Oxoprolinase Deficiency
PathBank:SMP0000722 Cystinosis, Ocular Nonnephropathic
PathBank:SMP0087278 Glutamate Metabolism
PathBank:SMP0087290 Cysteine Metabolism
PathBank:SMP0120705 Glutathione Synthetase Deficiency
PathBank:SMP0120785 gamma-Glutamyltranspeptidase Deficiency
PathBank:SMP0120873 Cystinosis, Ocular Nonnephropathic
PathBank:SMP0120564 beta-Mercaptolactate-Cysteine Disulfiduria
PathBank:SMP0000385 Homocarnosinosis
PathBank:SMP0000136 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0000499 beta-Mercaptolactate-Cysteine Disulfiduria
PathBank:SMP0000567 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0002317 Glutathione Metabolism
PathBank:SMP0087277 Glutathione Metabolism
PathBank:SMP0063607 Cysteine Metabolism
PathBank:SMP0120700 gamma-Glutamyltransferase Deficiency
PathBank:SMP0120716 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0120784 5-Oxoprolinase Deficiency
PathBank:SMP0120445 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120485 Glutathione Synthetase Deficiency
PathBank:SMP0120566 gamma-Glutamyltranspeptidase Deficiency
PathBank:SMP0122133 Glutathione Metabolism II
PathBank:SMP0122217 Glutathione Metabolism III
PathBank:SMP0000013 Cysteine Metabolism
PathBank:SMP0000337 Glutathione Synthetase Deficiency
PathBank:SMP0000501 gamma-Glutamyltranspeptidase Deficiency
PathBank:SMP0087428 Glutamate Metabolism
PathBank:SMP0063590 Glutamate Metabolism
PathBank:SMP0120659 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120667 5-Oxoprolinuria
PathBank:SMP0120783 beta-Mercaptolactate-Cysteine Disulfiduria
PathBank:SMP0120438 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120446 5-Oxoprolinuria
PathBank:SMP0120494 Homocarnosinosis
PathBank:SMP0120631 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000072 Glutamate Metabolism
PathBank:SMP0000243 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000143 5-Oxoprolinuria
PathBank:SMP0000183 gamma-Glutamyltransferase Deficiency
PathBank:SMP0087427 Glutathione Metabolism
PathBank:SMP0063622 Glutathione Metabolism