EC: 6.1.1.5

isoleucine-tRNA ligase (L-isoleucine:tRNAIle ligase (AMP-forming))

enzyme lambda metabolic reaction experiment
uniprot:P00956 [isoleucine---tRNA ligase(Enzyme) wildtype]

L_Isoleucine + ATP + tRNA(Ile) -> L_Isoleucyl_tRNA(Ile) + Diphosphate + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 5 mM MgCl2, 10 mM 2-mercaptoethanol
PH: 7.5
Temperature: 37
uniprot:P00956 [isoleucine---tRNA ligase(Enzyme) mutant G94R]

tRNA(Ile) + L_Isoleucine + ATP -> AMP + L_Isoleucyl_tRNA(Ile) + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 5 mM MgCl2, 10 mM 2-mercaptoethanol
PH: 7.5
Temperature: 37
uniprot:P00956 [isoleucine---tRNA ligase(Enzyme) wildtype]

Isoleucine + ATP -> Isoleucyl_AMP + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 5 mM MgCl2, 10 mM 2-mercaptoethanol
PH: 7.5
Temperature: 37
uniprot:P00956 [isoleucine---tRNA ligase(Enzyme) mutant G94R]

ATP + Isoleucine -> Diphosphate + Isoleucyl_AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 5 mM MgCl2, 10 mM 2-mercaptoethanol
PH: 7.5
Temperature: 37
uniprot:P00956 [isoleucine---tRNA ligase(Enzyme) wildtype]

ATP + Isoleucine -> Isoleucyl_AMP + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 5 mM MgCl2, 10 mM 2-mercaptoethanol
PH: 7.5
Temperature: 37
uniprot:P00956 [isoleucine---tRNA ligase(Enzyme) mutant G94R]

ATP + Isoleucine -> Diphosphate + Isoleucyl_AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM Tris-HCl, 5 mM MgCl2, 10 mM 2-mercaptoethanol
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:VCHO_TRNA-CHARGING-PWY tRNA charging
BioCyc:BTHE_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECO_TRNA-CHARGING-PWY tRNA charging
BioCyc:MTBH37RV_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOO157_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOL316407_TRNA-CHARGING-PWY tRNA charging
BioCyc:MTBCDC1551_TRNA-CHARGING-PWY tRNA charging
BioCyc:HPY_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOL199310_TRNA-CHARGING-PWY tRNA charging
BioCyc:CAULO_TRNA-CHARGING-PWY tRNA charging
BioCyc:AGRO_TRNA-CHARGING-PWY tRNA charging
BioCyc:BSUB_TRNA-CHARGING-PWY tRNA charging
BioCyc:GCF_000013425_TRNA-CHARGING-PWY tRNA charging
BioCyc:HUMAN_TRNA-CHARGING-PWY tRNA charging
BioCyc:META_TRNA-CHARGING-PWY tRNA charging
BioCyc:YEAST_TRNA-CHARGING-PWY tRNA charging
BioCyc:MOUSE_TRNA-CHARGING-PWY tRNA charging pathway
BioCyc:CORYNE_TRNA-CHARGING-PWY tRNA charging
PathBank:SMP0000820 tRNA Charging
PathBank:SMP0000824 tRNA Charging 2
PathBank:SMP0001887 Operon: tRNA Synthetase & Peptidase
Reactome:R-DDI-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-MMU-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-DDI-9730414 MITF-M-regulated melanocyte development
Reactome:R-HSA-9856649 Transcriptional and post-translational regulation of MITF-M expression and activity
Reactome:R-MMU-9730414 MITF-M-regulated melanocyte development
Reactome:R-HSA-9730414 MITF-M-regulated melanocyte development
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-379726 Mitochondrial tRNA aminoacylation
Reactome:R-DDI-1266738 Developmental Biology
Reactome:R-HSA-1266738 Developmental Biology
Reactome:R-HSA-379716 Cytosolic tRNA aminoacylation
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-72766 Translation
Reactome:R-MMU-1266738 Developmental Biology
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2408522 Selenoamino acid metabolism
Reactome:R-HSA-379724 tRNA Aminoacylation
PathBank:SMP0121283 tRNA Charging 2
PathBank:SMP0121281 tRNA Charging
PathBank:SMP0111872 Protein Synthesis: Isoleucine
PathBank:SMP0119315 Protein Synthesis: Isoleucine