EC: 6.1.1.21

histidine-tRNA ligase (L-histidine:tRNAHis ligase (AMP-forming))

enzyme lambda metabolic reaction experiment
uniprot:P60906 [histidine---tRNA ligase(Enzyme) wildtype]

ATP + L_Histidine + tRNA(His) -> Diphosphate + L_Histidyl_tRNA(His) + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 100 mM KCl, 8.3 mM DTT, 10 mM MgCl
PH: 7.5
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) wildtype]

tRNA(His) + L_Histidine + ATP -> L_Histidyl_tRNA(His) + Diphosphate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 100 mM KCl, 8.3 mM DTT, 10 mM MgCl
PH: 7.5
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) wildtype]

ATP + L_Histidine + tRNA(His) -> AMP + L_Histidyl_tRNA(His) + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 100 mM KCl, 8.3 mM DTT, 10 mM MgCl
PH: 7.5
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant NcatHisRS]

tRNA(His) + L_Histidine + ATP -> AMP + L_Histidyl_tRNA(His) + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 100 mM KCl, 8.3 mM DTT, 10 mM MgCl
PH: 7.5
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant NcatHisRS]

tRNA(His) + ATP + L_Histidine -> L_Histidyl_tRNA(His) + Diphosphate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 100 mM KCl, 8.3 mM DTT, 10 mM MgCl
PH: 7.5
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant NcatHisRS]

L_Histidine + tRNA(His) + ATP -> AMP + Diphosphate + L_Histidyl_tRNA(His)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 100 mM KCl, 8.3 mM DTT, 10 mM MgCl
PH: 7.5
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) wildtype]

L_Histidine + tRNA(His) + ATP -> AMP + Diphosphate + L_Histidyl_tRNA(His)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 100 mM KCl, 8.3 mM DTT, 10 mM MgCl
PH: 7.5
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant NcatHisRS]

ATP + tRNA(His) + L_Histidine -> AMP + Diphosphate + L_Histidyl_tRNA(His)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 100 mM KCl, 8.3 mM DTT, 10 mM MgCl
PH: 7.5
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) wildtype]

tRNA(His) + ATP + L_Histidine -> L_Histidyl_tRNA(His) + Diphosphate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 10 mM beta-mercaptoethanol, 10 mM KF, 10 mM MgCl2, 2 mM [32P]NaPPi
PH: 8
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant (His6)-HisRS]

L_Histidine + tRNA(His) + ATP -> Diphosphate + AMP + L_Histidyl_tRNA(His)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 10 mM beta-mercaptoethanol, 10 mM KF, 10 mM MgCl2, 2 mM [32P]NaPPi
PH: 8
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant NcatHisRS]

ATP + tRNA(His) + L_Histidine -> L_Histidyl_tRNA(His) + AMP + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 10 mM beta-mercaptoethanol, 10 mM KF, 10 mM MgCl2, 2 mM [32P]NaPPi
PH: 8
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant NcatHisRS]

ATP + L_Histidine + tRNA(His) -> L_Histidyl_tRNA(His) + Diphosphate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 10 mM beta-mercaptoethanol, 10 mM KF, 10 mM MgCl2, 2 mM [32P]NaPPi
PH: 8
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) wildtype]

tRNA(His) + L_Histidine + ATP -> Diphosphate + L_Histidyl_tRNA(His) + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 10 mM beta-mercaptoethanol, 10 mM KF, 10 mM MgCl2, 2 mM [32P]NaPPi
PH: 8
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant (His6)-HisRS]

ATP + tRNA(His) + L_Histidine -> L_Histidyl_tRNA(His) + AMP + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 10 mM beta-mercaptoethanol, 10 mM KF, 10 mM MgCl2, 2 mM [32P]NaPPi
PH: 8
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant CterHisRS]

L_Histidine + ATP + tRNA(His) -> Diphosphate + AMP + L_Histidyl_tRNA(His)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 100 mM KCl, 8.3 mM DTT, 10 mM MgCl
PH: 7.5
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant V-HisRS-R259K]

tRNA(His) + L_Histidine + ATP -> L_Histidyl_tRNA(His) + AMP + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 50 mM betaME, 2.6 mM [32P]-Na2P2O7
PH: 7.4
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) wildtype]

ATP + L_Histidine + tRNA(His) -> L_Histidyl_tRNA(His) + AMP + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 50 mM betaME, 2.6 mM [32P]-Na2P2O7
PH: 7.4
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant des(A2-Q6)-HisRS]

tRNA(His) + ATP + L_Histidine -> L_Histidyl_tRNA(His) + Diphosphate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 50 mM betaME, 2.6 mM [32P]-Na2P2O7
PH: 7.4
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant des(A2-G10)-HisRS]

L_Histidine + tRNA(His) + ATP -> L_Histidyl_tRNA(His) + Diphosphate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 50 mM betaME, 2.6 mM [32P]-Na2P2O7
PH: 7.4
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant V-HisRS]

tRNA(His) + L_Histidine + ATP -> AMP + L_Histidyl_tRNA(His) + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 50 mM betaME, 2.6 mM [32P]-Na2P2O7
PH: 7.4
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant M-HisRS]

tRNA(His) + L_Histidine + ATP -> L_Histidyl_tRNA(His) + Diphosphate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 50 mM betaME, 2.6 mM [32P]-Na2P2O7
PH: 7.4
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant V-HisRS-R259Q]

tRNA(His) + L_Histidine + ATP -> AMP + Diphosphate + L_Histidyl_tRNA(His)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 50 mM betaME, 2.6 mM [32P]-Na2P2O7
PH: 7.4
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant M-HisRS]

L_Histidine + ATP + tRNA(His) -> L_Histidyl_tRNA(His) + Diphosphate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 50 mM betaME, 2.6 mM [32P]-Na2P2O7
PH: 7.4
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant V-HisRS]

L_Histidine + ATP + tRNA(His) -> Diphosphate + L_Histidyl_tRNA(His) + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 50 mM betaME, 2.6 mM [32P]-Na2P2O7
PH: 7.4
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant des(A2-Q6)-HisRS]

tRNA(His) + L_Histidine + ATP -> AMP + L_Histidyl_tRNA(His) + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 50 mM betaME, 2.6 mM [32P]-Na2P2O7
PH: 7.4
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) wildtype]

tRNA(His) + L_Histidine + ATP -> AMP + L_Histidyl_tRNA(His) + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 50 mM betaME, 2.6 mM [32P]-Na2P2O7
PH: 7.4
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant des(A2-G10)-HisRS]

tRNA(His) + L_Histidine + ATP -> Diphosphate + AMP + L_Histidyl_tRNA(His)		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 50 mM betaME, 2.6 mM [32P]-Na2P2O7
PH: 7.4
Temperature: 37
uniprot:P60906 [histidine---tRNA ligase(Enzyme) mutant V-HisRS-R259K]

ATP + tRNA(His) + L_Histidine -> AMP + L_Histidyl_tRNA(His) + Diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 50 mM betaME, 2.6 mM [32P]-Na2P2O7
PH: 7.4
Temperature: 37
uniprot:O52765 [histidine---tRNA ligase(Enzyme) mutant S2280]

L_Histidine + ATP + tRNA(His) -> Diphosphate + AMP + L_Histidyl_tRNA(His)		 (( (V * Kc) ) / (C + Kc)) buffer: 0.1 M sodium cacodylate, 24 mM MgCl2, 0.2 mM 2-mercaptoethanol, 100 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
uniprot:O52765 [histidine---tRNA ligase(Enzyme) mutant S1587]

tRNA(His) + ATP + L_Histidine -> Diphosphate + L_Histidyl_tRNA(His) + AMP		 (( (V * Kc) ) / (C + Kc)) buffer: 0.1 M sodium cacodylate, 24 mM MgCl2, 0.2 mM 2-mercaptoethanol, 100 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
uniprot:O52765 [histidine---tRNA ligase(Enzyme) wildtype]

ATP + tRNA(His) + L_Histidine -> L_Histidyl_tRNA(His) + AMP + Diphosphate		 (( (V * Kc) ) / (C + Kc)) buffer: 0.1 M sodium cacodylate, 24 mM MgCl2, 0.2 mM 2-mercaptoethanol, 100 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
uniprot:O52765 [histidine---tRNA ligase(Enzyme) mutant S1520]

L_Histidine + ATP + tRNA(His) -> AMP + L_Histidyl_tRNA(His) + Diphosphate		 (( (V * Kc) ) / (C + Kc)) buffer: 0.1 M sodium cacodylate, 24 mM MgCl2, 0.2 mM 2-mercaptoethanol, 100 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
uniprot:O52765 [histidine---tRNA ligase(Enzyme) mutant S1595]

L_Histidine + tRNA(His) + ATP -> Diphosphate + L_Histidyl_tRNA(His) + AMP		 (( (V * Kc) ) / (C + Kc)) buffer: 0.1 M sodium cacodylate, 24 mM MgCl2, 0.2 mM 2-mercaptoethanol, 100 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
uniprot:O52765 [histidine---tRNA ligase(Enzyme) mutant S2280]

ATP + L_Histidine + tRNA(His) -> Diphosphate + L_Histidyl_tRNA(His) + AMP		 (( (V * Kb) ) / (B + Kb)) buffer: 0.1 M sodium cacodylate, 8 mM MgCl2, 0.2 mM 2-mercaptoethanol, 100 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
uniprot:O52765 [histidine---tRNA ligase(Enzyme) mutant S1595]

ATP + tRNA(His) + L_Histidine -> AMP + L_Histidyl_tRNA(His) + Diphosphate		 (( (V * Kb) ) / (B + Kb)) buffer: 0.1 M sodium cacodylate, 8 mM MgCl2, 0.2 mM 2-mercaptoethanol, 100 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
uniprot:O52765 [histidine---tRNA ligase(Enzyme) mutant S1520]

L_Histidine + tRNA(His) + ATP -> AMP + Diphosphate + L_Histidyl_tRNA(His)		 (( (V * Kb) ) / (B + Kb)) buffer: 0.1 M sodium cacodylate, 8 mM MgCl2, 0.2 mM 2-mercaptoethanol, 100 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
uniprot:O52765 [histidine---tRNA ligase(Enzyme) mutant S1587]

ATP + L_Histidine + tRNA(His) -> AMP + L_Histidyl_tRNA(His) + Diphosphate		 (( (V * Kb) ) / (B + Kb)) buffer: 0.1 M sodium cacodylate, 8 mM MgCl2, 0.2 mM 2-mercaptoethanol, 100 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
- [histidine---tRNA ligase(Enzyme) mutant S1210]

tRNA(His) + ATP + L_Histidine -> Diphosphate + L_Histidyl_tRNA(His) + AMP		 (( (V * Kc) ) / (C + Kc)) buffer: 0.1 M sodium cacodylate, 24 mM MgCl2, 0.2 mM 2-mercaptoethanol, 100 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
- [histidine---tRNA ligase(Enzyme) mutant S1210]

L_Histidine + ATP + tRNA(His) -> L_Histidyl_tRNA(His) + Diphosphate + AMP		 (( (V * Kb) ) / (B + Kb)) buffer: 0.1 M sodium cacodylate, 8 mM MgCl2, 0.2 mM 2-mercaptoethanol, 100 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
uniprot:O52765 [histidine---tRNA ligase(Enzyme) wildtype]

tRNA(His) + ATP + L_Histidine -> AMP + L_Histidyl_tRNA(His) + Diphosphate		 (( (V * Ka) ) / (A + Ka)) buffer: 0.1 M sodium cacodylate, 24 mM MgCl2, 0.2 mM 2-mercaptoethanol, 100 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
uniprot:O52765 [histidine---tRNA ligase(Enzyme) wildtype]

L_Histidine + ATP + tRNA(His) -> Diphosphate + AMP + L_Histidyl_tRNA(His)		 (( (V * Kb) ) / (B + Kb)) buffer: 0.1 M sodium cacodylate, 8 mM MgCl2, 0.2 mM 2-mercaptoethanol, 100 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
uniprot:O52765 [histidine---tRNA ligase(Enzyme) mutant S1595]

tRNA(His) + L_Histidine + ATP -> AMP + Diphosphate + L_Histidyl_tRNA(His)		 (( (V * Ka) ) / (A + Ka)) buffer: 0.1 M sodium cacodylate, 24 mM MgCl2, 0.2 mM 2-mercaptoethanol, 100 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
uniprot:O52765 [histidine---tRNA ligase(Enzyme) mutant S1587]

tRNA(His) + L_Histidine + ATP -> L_Histidyl_tRNA(His) + AMP + Diphosphate		 (( (V * Ka) ) / (A + Ka)) buffer: 0.1 M sodium cacodylate, 24 mM MgCl2, 0.2 mM 2-mercaptoethanol, 100 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
uniprot:O52765 [histidine---tRNA ligase(Enzyme) mutant S1520]

L_Histidine + tRNA(His) + ATP -> L_Histidyl_tRNA(His) + AMP + Diphosphate		 (( (V * Ka) ) / (A + Ka)) buffer: 0.1 M sodium cacodylate, 24 mM MgCl2, 0.2 mM 2-mercaptoethanol, 100 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
uniprot:O52765 [histidine---tRNA ligase(Enzyme) mutant S2280]

L_Histidine + tRNA(His) + ATP -> Diphosphate + L_Histidyl_tRNA(His) + AMP		 (( (V * Ka) ) / (A + Ka)) buffer: 0.1 M sodium cacodylate, 24 mM MgCl2, 0.2 mM 2-mercaptoethanol, 100 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37
- [histidine---tRNA ligase(Enzyme) mutant S1210]

L_Histidine + tRNA(His) + ATP -> L_Histidyl_tRNA(His) + AMP + Diphosphate		 (( (V * Ka) ) / (A + Ka)) buffer: 0.1 M sodium cacodylate, 24 mM MgCl2, 0.2 mM 2-mercaptoethanol, 100 µg/ml bovine serum albumin
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:VCHO_TRNA-CHARGING-PWY tRNA charging
BioCyc:ANTHRA_TRNA-CHARGING-PWY tRNA charging
BioCyc:BTHE_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECO_TRNA-CHARGING-PWY tRNA charging
BioCyc:MTBH37RV_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOO157_TRNA-CHARGING-PWY tRNA charging
BioCyc:SYNEL_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOL316407_TRNA-CHARGING-PWY tRNA charging
BioCyc:PDIF272563_TRNA-CHARGING-PWY tRNA charging
BioCyc:MTBCDC1551_TRNA-CHARGING-PWY tRNA charging
BioCyc:HPY_TRNA-CHARGING-PWY tRNA charging
BioCyc:ECOL199310_TRNA-CHARGING-PWY tRNA charging
BioCyc:CAULO_TRNA-CHARGING-PWY tRNA charging
BioCyc:AGRO_TRNA-CHARGING-PWY tRNA charging
BioCyc:BSUB_TRNA-CHARGING-PWY tRNA charging
BioCyc:GCF_000013425_TRNA-CHARGING-PWY tRNA charging
BioCyc:HUMAN_TRNA-CHARGING-PWY tRNA charging
BioCyc:META_TRNA-CHARGING-PWY tRNA charging
BioCyc:YEAST_TRNA-CHARGING-PWY tRNA charging
BioCyc:MOUSE_TRNA-CHARGING-PWY tRNA charging pathway
BioCyc:CORYNE_TRNA-CHARGING-PWY tRNA charging
PathBank:SMP0000820 tRNA Charging
PathBank:SMP0000824 tRNA Charging 2
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-379726 Mitochondrial tRNA aminoacylation
Reactome:R-HSA-379716 Cytosolic tRNA aminoacylation
Reactome:R-HSA-72766 Translation
Reactome:R-HSA-379724 tRNA Aminoacylation
PathBank:SMP0121283 tRNA Charging 2
PathBank:SMP0111871 Protein Synthesis: Histidine
PathBank:SMP0119314 Protein Synthesis: Histidine
PathBank:SMP0120493 Histidinemia
PathBank:SMP0121281 tRNA Charging
PathBank:SMP0000044 Histidine Metabolism
PathBank:SMP0000191 Histidinemia
PathBank:SMP0087219 Histidine Metabolism
PathBank:SMP0063632 Histidine Metabolism
PathBank:SMP0119392 Protein Synthesis: Histidine