EC: 5.1.3.1

ribulose-phosphate 3-epimerase (D-ribulose-5-phosphate 3-epimerase)

enzyme lambda metabolic reaction experiment
uniprot:Q43157 [ribulose-phosphate 3-epimerase(Enzyme) wildtype]

D_Ribulose_5_phosphate -> D_Xylulose_5_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 160 mM Tris/HCl, 5 mM MgCl2, 0.3 mM NADH, 0.5 mM thiamine diphosphate, 4 mM sodium glutathione, 0.5 mM Na2HAsO4, 3 mM ribose 5-phosphate, 1 U transketolase, 8 U alpha-glycerophosphate dehydrogenase/ triose phosphate isomerase (1:1 mixture)
PH: 7.5
Temperature: 36
uniprot:Q43843 [ribulose-phosphate 3-epimerase(Enzyme) wildtype N-terminal His-tag]

D_Ribulose_5_phosphate -> D_Xylulose_5_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 160 mM Tris-HCl, 5 mM MgCl2, 0.3 mM NADH, 0.5 mM thiamine diphosphate, 4 mM sodium glutathione, 0.5 mM Na2HAsO4, 3 mM ribose 5-phosphate, 1 U transketolase, 8 U alpha-glycerophosphate dehydrogenase/ triose phosphate isomerase (1:1 mixture)
PH: 8
Temperature: 25
uniprot:Q9SE42 [ribulose-phosphate 3-epimerase(Enzyme) wildtype N-terminal His-tag]

D_Ribulose_5_phosphate -> D_Xylulose_5_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 160 mM Tris-HCl, 5 mM MgCl2, 0.3 mM NADH, 0.5 mM thiamine diphosphate, 4 mM sodium glutathione, 0.5 mM Na2HAsO4, 3 mM ribose 5-phosphate, 1 U transketolase, 8 U alpha-glycerophosphate dehydrogenase/ triose phosphate isomerase (1:1 mixture)
PH: 8
Temperature: 25

Pathways

pathway id name
BioCyc:ECO_PENTOSE-P-PWY pentose phosphate pathway
BioCyc:ARA_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:ARA_PENTOSE-P-PWY pentose phosphate pathway
BioCyc:ARA_CALVIN-PWY Calvin-Benson-Bassham cycle
BioCyc:HPY_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:ECOO157_PWY-1861 formaldehyde assimilation II (RuMP Cycle)
BioCyc:ECOL199310_PWY-1861 formaldehyde assimilation II (RuMP Cycle)
BioCyc:MOUSE_PENTOSE-P-PWY pentose phosphate pathway
BioCyc:BSUB_PENTOSE-P-PWY pentose phosphate pathway
BioCyc:HUMAN_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:ECO_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:YEAST_PENTOSE-P-PWY pentose phosphate pathway
BioCyc:ECOL199310_PWY-6901 glucose and xylose degradation
BioCyc:BSUB_PWY-1861 formaldehyde assimilation II (assimilatory RuMP Cycle)
BioCyc:ECOL316407_PENTOSE-P-PWY pentose phosphate pathway
BioCyc:META_PWY-7218 photosynthetic 3-hydroxybutanoate biosynthesis (engineered)
BioCyc:META_PENTOSE-P-PWY pentose phosphate pathway
BioCyc:MTBH37RV_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:MTBH37RV_PENTOSE-P-PWY pentose phosphate pathway
BioCyc:MTBCDC1551_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:ECOO157_P21-PWY pentose phosphate pathway (partial)
BioCyc:ECOO157_PENTOSE-P-PWY pentose phosphate pathway
BioCyc:ECOO157_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:ECOL199310_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:ECOL199310_P21-PWY pentose phosphate pathway (partial)
BioCyc:ECOL316407_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:META_P21-PWY pentose phosphate pathway (partial)
BioCyc:META_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:YEAST_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:ARA_PWY-5723 Rubisco shunt
BioCyc:ARA_PHOTOALL-PWY oxygenic photosynthesis
BioCyc:ECOO157_PWY-6901 glucose and xylose degradation
BioCyc:ECOL199310_PENTOSE-P-PWY pentose phosphate pathway
BioCyc:MOUSE_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:BSUB_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
PlantCyc:ARA_PENTOSE-P-PWY pentose phosphate pathway
PlantCyc:ARA_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
PlantCyc:ARA_PWY-5723 Rubisco shunt
PlantCyc:ARA_CALVIN-PWY Calvin-Benson-Bassham cycle
PlantCyc:ARA_PHOTOALL-PWY oxygenic photosynthesis
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SCE-71387 Carbohydrate metabolism
Reactome:R-SCE-71336 Pentose phosphate pathway
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-MMU-71387 Carbohydrate metabolism
Reactome:R-SPO-71387 Carbohydrate metabolism
Reactome:R-SPO-71336 Pentose phosphate pathway
Reactome:R-MMU-71336 Pentose phosphate pathway
WikiPathways:WP5220 Metabolic reprogramming in pancreatic cancer
PathBank:SMP0120585 Transaldolase Deficiency
PathBank:SMP0000520 Transaldolase Deficiency
PathBank:SMP0002406 Riboneogenesis
PathBank:SMP0063588 Pentose Phosphate Pathway
PathBank:SMP0120584 Ribose-5-phosphate Isomerase Deficiency
PathBank:SMP0000519 Ribose-5-phosphate Isomerase Deficiency
PathBank:SMP0002345 Xylitol Degradation
PathBank:SMP0000031 Pentose Phosphate Pathway
PathBank:SMP0120583 Glucose-6-phosphate Dehydrogenase Deficiency
PathBank:SMP0000518 Glucose-6-phosphate Dehydrogenase Deficiency