EC: 4.3.1.23

tyrosine ammonia-lyase (L-tyrosine ammonia-lyase (trans-p-hydroxycinnamate-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q3IWB0 [tyrosine ammonia-lyase(Enzyme) mutant H89N His6-tagged]

L_Tyrosine -> NH3 + 4_Coumarate
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM bis-Tris propane or 100 mM 2-amino-2-methyl-1,3-propanediol
PH: 9
Temperature: 37
uniprot:Q3IWB0 [tyrosine ammonia-lyase(Enzyme) wildtype His6-tagged]

L_Tyrosine -> 4_Coumarate + NH3
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM bis-Tris propane or 100 mM 2-amino-2-methyl-1,3-propanediol
PH: 9
Temperature: 37
uniprot:P35510 [tyrosine ammonia-lyase(Enzyme) wildtype His6-tagged]

L_Tyrosine -> NH3 + 4_Coumarate
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM bis-Tris propane or 100 mM 2-amino-2-methyl-1,3-propanediol
PH: 9
Temperature: 37
uniprot:P35510 [tyrosine ammonia-lyase(Enzyme) mutant F144H His6-tagged]

L_Tyrosine -> 4_Coumarate + NH3
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM bis-Tris propane or 100 mM 2-amino-2-methyl-1,3-propanediol
PH: 9
Temperature: 37
uniprot:Q3IWB0 [tyrosine ammonia-lyase(Enzyme) mutant H89F His6-tagged]

L_Tyrosine -> NH3 + 4_Coumarate
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM bis-Tris propane or 100 mM 2-amino-2-methyl-1,3-propanediol
PH: 9
Temperature: 37
uniprot:Q3IWB0 [tyrosine ammonia-lyase(Enzyme) mutant H89Q His6-tagged]

L_Tyrosine -> NH3 + 4_Coumarate
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM bis-Tris propane or 100 mM 2-amino-2-methyl-1,3-propanediol
PH: 9
Temperature: 37
uniprot:Q3IWB0 [tyrosine ammonia-lyase(Enzyme) wildtype]

L_Tyrosine -> NH3 + 4_Coumarate
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M TrisHCl
PH: 8.5
Temperature: 37
uniprot:Q3IWB0 [tyrosine ammonia-lyase(Enzyme) mutant H89F]

L_Phenylalanine -> trans_Cinnamate + NH3
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M TrisHCl
PH: 8.5
Temperature: 37
uniprot:Q3IWB0 [tyrosine ammonia-lyase(Enzyme) wildtype]

L_Phenylalanine -> NH3 + trans_Cinnamate
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M TrisHCl
PH: 8.5
Temperature: 37
uniprot:Q1LRV9 [tyrosine ammonia-lyase(Enzyme) wildtype]

L_Tyrosine -> NH3 + 4_Coumarate
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 mM Tris
PH: 8.8
Temperature: 30
uniprot:Q0VZ68 [tyrosine ammonia-lyase(Enzyme) wildtype]

L_Tyrosine -> 4_Coumarate + NH3
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 mM Tris
PH: 8.8
Temperature: 30
uniprot:Q0VZ68 [tyrosine ammonia-lyase(Enzyme) mutant MIYMLV (62-67)]

L_Tyrosine -> 4_Coumarate + NH3
(( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 mM Tris
PH: 8.8
Temperature: 30
uniprot:Q8GMG0 [tyrosine ammonia-lyase(Enzyme) wildtype N-terminal His6-tagged]

L_Tyrosine -> NH3 + 4_Coumarate
(( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 8.8
Temperature: 25

Pathways

pathway id name