EC: 4.2.3.108

1,8-cineole synthase (geranyl-diphosphate diphosphate-lyase (cyclizing, 1,8-cineole-forming))

enzyme lambda metabolic reaction experiment
uniprot:P0DI77 [1,8-cineole synthase(Enzyme) wildtype AtTPS-Cin/At3g25830]

Geranyl_diphosphate + H2O -> 1,8_Cineole + Diphosphate
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM 3-(N-Morpholino)-2-hydroxypropanesulfonic acid, 10% (v/v) Glycerol, 1 mM DTT, 0.2 mM NaWO4, 0.1 mM NaF, 40 mM Mg2+ or 1 mM Mn2+
PH: 7
Temperature: 30
uniprot:P0DI76 [1,8-cineole synthase(Enzyme) wildtype AtTPS-Cin/At3g25820]

Geranyl_diphosphate + H2O -> Diphosphate + 1,8_Cineole
(( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM 3-(N-Morpholino)-2-hydroxypropanesulfonic acid, 10% (v/v) Glycerol, 1 mM DTT, 0.2 mM NaWO4, 0.1 mM NaF, 40 mM Mg2+ or 1 mM Mn2+
PH: 7
Temperature: 30
uniprot:O81191 [Mg2+]

Geranyl_diphosphate + H2O -> 1,8_Cineole + Diphosphate
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM MOPSO, 5 mM DTT, 10% (v/v) glycerol
PH: 7.1
Temperature: 31

Pathways

pathway id name
PathBank:SMP0014204 Monoterpenoid Biosynthesis