EC: 4.2.1.91

arogenate dehydratase (L-arogenate hydro-lyase (decarboxylating; L-phenylalanine-forming))

enzyme lambda metabolic reaction experiment
- [L-Tyrosine]

L_Arogenate -> CO2 + L_Phenylalanine + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 0.2 M 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid, 1 µM Leupeptin, 1 µM Pepstatin
PH: 9
Temperature: 33
- [L-Tyrosine]

L_Arogenate -> L_Phenylalanine + H2O + CO2		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid, 1 µM Leupeptin, 1 µM Pepstatin
PH: 9
Temperature: 32
- [arogenate dehydratase(Enzyme) wildtype]

L_Arogenate -> CO2 + H2O + L_Phenylalanine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate, 1.0 mM DTT
PH: 7.2
Temperature: 37
- [arogenate dehydratase(Enzyme) wildtype]

Prephenate -> H2O + Phenylpyruvate + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Potassium phosphate, 1.0 mM DTT
PH: 7.2
Temperature: 37
- [Tyrosine]

L_Arogenate -> CO2 + L_Phenylalanine + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Epps, 20% Ethylene glycol, 0.1 mM EDTA, 2 mM DTT
PH: 8
Temperature: 22
- [arogenate dehydratase(Enzyme) wildtype]

L_Arogenate -> H2O + L_Phenylalanine + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Epps, 20% Ethylene glycol, 0.1 mM EDTA, 2 mM DTT
PH: 8
Temperature: 22
- [arogenate dehydratase(Enzyme) wildtype]

L_Arogenate -> L_Phenylalanine + H2O + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Epps, 20% Ethylene glycol, 0.1 mM EDTA, 2 mM DTT
PH: 8
Temperature: 22

Pathways

pathway id name
BioCyc:ARA_PWY-3481 superpathway of L-phenylalanine and L-tyrosine biosynthesis
BioCyc:ARA_PWY-7432 L-phenylalanine biosynthesis III (cytosolic, plants)
BioCyc:ARA_COMPLETE-ARO-ARA-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
BioCyc:ARA_PWY-3462 L-phenylalanine biosynthesis II
PlantCyc:ARA_COMPLETE-ARO-ARA-PWY superpathway of phenylalanine, tyrosine and tryptophan biosynthesis
PlantCyc:ARA_PWY-3481 superpathway of L-phenylalanine and L-tyrosine biosynthesis
PlantCyc:ARA_PWY-7432 L-phenylalanine biosynthesis III (cytosolic, plants)
PlantCyc:ARA_PWY-3462 L-phenylalanine biosynthesis II
PlantCyc:PLANT_PWY-3462 L-phenylalanine biosynthesis II
PlantCyc:PLANT_PWY-3481 superpathway of L-phenylalanine and L-tyrosine biosynthesis
PathBank:SMP0002442 Phenylalanine Metabolism