EC: 4.2.1.35

(R)-2-methylmalate dehydratase ((R)-2-methylmalate hydro-lyase (2-methylmaleate-forming))

enzyme lambda metabolic reaction experiment
- [(R)-2-methylmalate dehydratase(Enzyme) wildtype MJ0499/MJ1277]

2_Methylmaleate + H2O -> D_Citramalate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM TES-KOH, 300 mM KCl, 10 mM MgCl2
PH: 7.5
Temperature: 60
- [(R)-2-methylmalate dehydratase(Enzyme) wildtype MJ0499/MJ1277]

D_Citramalate -> 2_Methylmaleate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM TES-KOH, 300 mM KCl, 10 mM MgCl2
PH: 7.5
Temperature: 60
- [(R)-2-methylmalate dehydratase(Enzyme) wildtype MJ0499/MJ1277]

2_Methylmaleate + H2O -> D_Citramalate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM TES-KOH, 300 mM KCl, 10 mM MgCl2, 5 mM NAD+, 30 µg/ml Isopropylmalate dehydrogenase from Methanococcus jannaschii
PH: 7.5
Temperature: 60

Pathways

pathway id name
BioCyc:META_LEUSYN-PWY L-leucine biosynthesis
BioCyc:META_PWY-5101 L-isoleucine biosynthesis II