- [Thiamine diphosphate]
3_Phosphonopyruvate -> 2_Phosphonoacetaldehyde + CO2 |
(( (( (E * kcat) ) * M) ) / (Km + M)) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 1 mM MnCl2, 0.15 mM NADH
PH: 7.3
Temperature: 25 |
- [Mn2+]
3_Phosphonopyruvate -> CO2 + 2_Phosphonoacetaldehyde |
(( (( (E * kcat) ) * M) ) / (Km + M)) |
|
buffer: 50 mM HEPES, 0.15 mM NADH, 1.5 mM thiamine pyrophosphate
PH: 7.3
Temperature: 25 |
- [Mg2+]
3_Phosphonopyruvate -> 2_Phosphonoacetaldehyde + CO2 |
(( (( (E * kcat) ) * M) ) / (Km + M)) |
|
buffer: 50 mM HEPES, 0.15 mM NADH, 1.5 mM thiamine pyrophosphate
PH: 7.3
Temperature: 25 |
- [Ca2+]
3_Phosphonopyruvate -> 2_Phosphonoacetaldehyde + CO2 |
(( (( (E * kcat) ) * M) ) / (Km + M)) |
|
buffer: 50 mM HEPES, 0.15 mM NADH, 1.5 mM thiamine pyrophosphate
PH: 7.3
Temperature: 25 |
- [phosphonopyruvate decarboxylase(Enzyme) wildtype]
3_Phosphonopyruvate -> CO2 + 2_Phosphonoacetaldehyde |
(( (E * kcat) ) / (( (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKa)))) ) + (( (10 ^ (0 - pKb)) ) / (10 ^ (0 - pH))))) |
|
buffer: 50 mM MES (pH 6.0-6.8) / 50 mM HEPES (pH 6.8-8.0) / 50 mM TAPS (pH 8.0-8.7), 5 mM MgCl2, 1 mM MnCl2, 75 mM chloride, 0.15 mM NADH, 1.5 mM thiamine pyrophosphate
PH: 6
Temperature: 25 |
- [phosphonopyruvate decarboxylase(Enzyme) mutant D260A]
3_Phosphonopyruvate -> CO2 + 2_Phosphonoacetaldehyde |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 1 mM MnCl2, 0.15 mM NADH, 1.5 mM thiamine pyrophosphate
PH: 7.3
Temperature: 25 |
- [phosphonopyruvate decarboxylase(Enzyme) wildtype]
3_Phosphonopyruvate -> CO2 + 2_Phosphonoacetaldehyde |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 1 mM MnCl2, 0.15 mM NADH, 1.5 mM thiamine pyrophosphate
PH: 7.3
Temperature: 25 |
- [phosphonopyruvate decarboxylase(Enzyme) mutant E213A]
3_Phosphonopyruvate -> CO2 + 2_Phosphonoacetaldehyde |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 1 mM MnCl2, 0.15 mM NADH, 1.5 mM thiamine pyrophosphate
PH: 7.3
Temperature: 25 |
- [phosphonopyruvate decarboxylase(Enzyme) mutant D258A]
3_Phosphonopyruvate -> 2_Phosphonoacetaldehyde + CO2 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 1 mM MnCl2, 0.15 mM NADH, 1.5 mM thiamine pyrophosphate
PH: 7.3
Temperature: 25 |
- [3-Sulfopyruvate]
3_Phosphonopyruvate -> CO2 + 2_Phosphonoacetaldehyde |
(1 / (( (( (Km * I) ) / (( (( (E * kcat) ) * S) ) * Ki)) ) + (1 / (( (E * kcat) ) * (1 + (Km / S)))))) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 1 mM MnCl2, 0.15 mM NADH, 1.5 mM thiamine pyrophosphate
PH: 7
Temperature: 25 |
- [2-Phosphonoacetaldehyde]
Pyruvate -> Acetaldehyde + CO2 |
(1 / (( (( (Km * I) ) / (( (( (E * kcat) ) * S) ) * Ki)) ) + (1 / (( (E * kcat) ) * (1 + (Km / S)))))) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 1 mM MnCl2, 0.15 mM NADH, 1.5 mM thiamine pyrophosphate
PH: 7.3
Temperature: 25 |
- [phosphonopyruvate decarboxylase(Enzyme) wildtype]
Pyruvate -> Acetaldehyde + CO2 |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 1 mM MnCl2, 0.15 mM NADH, 1.5 mM thiamine pyrophosphate
PH: 7.3
Temperature: 25 |