EC: 4.1.1.64

2,2-dialkylglycine decarboxylase (pyruvate) (2,2-dialkylglycine carboxy-lyase (amino-transferring; L-alanine-forming))

enzyme lambda metabolic reaction experiment
uniprot:P16932 [Na+]

2_Amino_2_methylpropanoate + Pyruvate -> L_Alanine + CO2 + Acetone		 (( (( (kcat * E) ) * S) ) / (S + Km)) buffer: 60 mM Bicine-TMA-hydroxide, 0.1 M dipotassium succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 8.2
Temperature: 25
uniprot:P16932 [K+]

Pyruvate + 2_Amino_2_methylpropanoate -> CO2 + Acetone + L_Alanine		 (( (( (kcat * E) ) * S) ) / (S + Km)) buffer: 60 mM Bicine-TMA-hydroxide, 0.1 M dipotassium succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 8.2
Temperature: 25
uniprot:P16932 [Pyridoxal phosphate]

2_Amino_2_methylpropanoate + Pyruvate -> L_Alanine + CO2 + Acetone		 (( (( (kcat * E) ) * S) ) / (S + Km)) buffer: 60 mM Bicine-TMA-hydroxide, 0.1 M dipotassium succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 8.2
Temperature: 25
uniprot:P16932 [K+]

Pyruvate + 2_Amino_2_methylpropanoate -> Acetone + CO2 + L_Alanine		 (( (( (kcat * E) ) * S) ) / (S + Km)) buffer: 60 mM Bicine-TMA-hydroxide, 0.1 M dipotassium succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase, 0.32 mM 1-amino cyclopropane 1-carboxylate
PH: 8.2
Temperature: 25
uniprot:P16932 [K+]

2_Amino_2_methylpropanoate + Pyruvate -> L_Alanine + CO2 + Acetone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [Na+]

Pyruvate + 2_Amino_2_methylpropanoate -> CO2 + Acetone + L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [Li+]

Pyruvate + 2_Amino_2_methylpropanoate -> Acetone + CO2 + L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [Pyridoxal phosphate]

Pyruvate + 2_Amino_2_methylpropanoate -> CO2 + Acetone + L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [Pyridoxal phosphate]

2_Amino_2_methylpropanoate + Pyruvate -> L_Alanine + Acetone + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [NH4+]

Pyruvate + 2_Amino_2_methylpropanoate -> CO2 + Acetone + L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [Mg2+]

2_Amino_2_methylpropanoate + Pyruvate -> Acetone + L_Alanine + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [Ca2+]

2_Amino_2_methylpropanoate + Pyruvate -> Acetone + L_Alanine + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [Na+]

2_Amino_2_methylpropanoate + Pyruvate -> L_Alanine + Acetone + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [Cs+]

2_Amino_2_methylpropanoate + Pyruvate -> L_Alanine + Acetone + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [Pyridoxal phosphate]

2_Amino_2_methylpropanoate + Pyruvate -> CO2 + L_Alanine + Acetone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [Li+]

Pyruvate + 2_Amino_2_methylpropanoate -> L_Alanine + Acetone + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [Ba2+]

Pyruvate + 2_Amino_2_methylpropanoate -> CO2 + Acetone + L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [Pyridoxal phosphate]

Pyruvate + 2_Amino_2_methylpropanoate -> Acetone + L_Alanine + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [NH4+]

2_Amino_2_methylpropanoate + Pyruvate -> Acetone + CO2 + L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [Mg2+]

Pyruvate + 2_Amino_2_methylpropanoate -> Acetone + CO2 + L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [Ca2+]

Pyruvate + 2_Amino_2_methylpropanoate -> CO2 + Acetone + L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [Cs+]

Pyruvate + 2_Amino_2_methylpropanoate -> L_Alanine + Acetone + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [K+]

2_Amino_2_methylpropanoate + Pyruvate -> CO2 + Acetone + L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [Pyridoxal phosphate]

Pyruvate + 2_Amino_2_methylpropanoate -> Acetone + CO2 + L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [Ba2+]

Pyruvate + 2_Amino_2_methylpropanoate -> Acetone + L_Alanine + CO2		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36
uniprot:P16932 [Pyridoxal phosphate]

2_Amino_2_methylpropanoate + Pyruvate -> CO2 + L_Alanine + Acetone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM TEA-succinate, 200 µM NADPH, 1 unit/ml NADPH-dependent secondary alcohol dehydrogenase
PH: 7.8
Temperature: 36

Pathways

pathway id name