EC: 3.5.1.5

urease (urea amidohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:E0ZS48 [urease(Enzyme) wildtype]

H2O + Urea -> CO2 + 2 NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM HEPES
PH: 7.5
Temperature: 50
uniprot:P07374 [urease(Enzyme) wildtype]

Urea + H2O -> CO2 + 2 NH3		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.05 M Tris/HCl, 0.02 M NaCl, 0.01 M MnCl2
PH: 7.5
Temperature: 25
- [urease(Enzyme) wildtype]

Urea + H2O -> CO2 + 2 NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM sodium acetate, 12,5% ethanol (v/v)
PH: 4
Temperature: 37
- [urease(Enzyme) wildtype]

H2O + Urea -> 2 NH3 + CO2		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM sodium acetate, 12,5% ethanol (v/v)
PH: 4
Temperature: 20
- [urease(Enzyme) wildtype]

H2O + Urea -> CO2 + 2 NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM sodium citrate, 12,5% ethanol (v/v)
PH: 3
Temperature: 20
- [H+]

H2O + Urea -> 2 NH3 + CO2		 (( (( (( (( (Vmax * K1) ) * K2) ) * H) ) + (( (Vmax1 * K1) ) * (H ^ 2))) ) / (( (( (K1 + H) ) * (K2 + H)) ) * (K3 + H))) buffer: 25 mM MES, 0.5 mM EDTA
PH: 6.2
Temperature: 36
- [H+]

Urea + H2O -> 2 NH3 + CO2		 (( (( (( (( (Vmax * K1) ) * K2) ) * H) ) + (( (Vmax1 * K1) ) * (H ^ 2))) ) / (( (( (K1 + H) ) * (K2 + H)) ) * (K3 + H))) buffer: 25 mM MES, 0.5 mM EDTA
PH: 6.2
Temperature: 36
- [H+]

H2O + Urea -> CO2 + 2 NH3		 (( (( (( (( (Vmax * K1) ) * K2) ) * H) ) + (( (Vmax1 * K1) ) * (H ^ 2))) ) / (( (( (K1 + H) ) * (K2 + H)) ) * (K3 + H))) buffer: 25 mM MES, 0.5 mM EDTA
PH: 6.2
Temperature: 36
- [H+]

Urea + H2O -> 2 NH3 + CO2		 (( (( (( (( (Vmax * K1) ) * K2) ) * H) ) + (( (Vmax1 * K1) ) * (H ^ 2))) ) / (( (( (K1 + H) ) * (K2 + H)) ) * (K3 + H))) buffer: 25 mM MES, 0.5 mM EDTA
PH: 6.2
Temperature: 36
- [H+]

H2O + Urea -> 2 NH3 + CO2		 (( (( (Vmax * K1) ) * H) ) / (( (K1 + H) ) * (K2 + H))) buffer: 25 mM HEPES, 0.5 mM EDTA
PH: 7.75
Temperature: 36
- [urease(Enzyme) mutant R336Q]

Urea + H2O -> 2 NH3 + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM HEPES, 0.5 mM EDTA
PH: 7.75
Temperature: 36
- [urease(Enzyme) mutant D221A]

Urea + H2O -> 2 NH3 + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM MES
PH: 5.1
Temperature: 36
- [urease(Enzyme) mutant D221N]

Urea + H2O -> 2 NH3 + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES
PH: 7.75
Temperature: 36
- [urease(Enzyme) mutant H219Q]

H2O + Urea -> 2 NH3 + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM MES, 0.5 mM EDTA
PH: 6.2
Temperature: 36
- [urease(Enzyme) mutant H320A]

Urea + H2O -> 2 NH3 + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM MES, 0.5 mM EDTA
PH: 6.2
Temperature: 36
- [urease(Enzyme) mutant H320N]

H2O + Urea -> CO2 + 2 NH3		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM MES, 0.5 mM EDTA
PH: 6.2
Temperature: 36
- [urease(Enzyme) wildtype]

H2O + Urea -> CO2 + 2 NH3		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM HEPES, 0.5 mM EDTA
PH: 7.75
Temperature: 36
- [urease(Enzyme) mutant H320Q]

H2O + Urea -> 2 NH3 + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM MES, 0.5 mM EDTA
PH: 6.2
Temperature: 36
- [urease(Enzyme) mutant H219N]

Urea + H2O -> CO2 + 2 NH3		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM MES, 0.5 mM EDTA
PH: 6.2
Temperature: 36
- [urease(Enzyme) mutant H219A]

Urea + H2O -> CO2 + 2 NH3		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM HEPES, 0.5 mM EDTA
PH: 7.75
Temperature: 36
- [H+]

Urea + H2O -> 2 NH3 + CO2		 (( (( (( (( (Vmax * K1) ) * K2) ) * H) ) + (( (Vmax1 * K1) ) * (H ^ 2))) ) / (( (( (K1 + H) ) * (K2 + H)) ) * (K3 + H))) buffer: 25 mM MES, 0.5 mM EDTA
PH: 6.2
Temperature: 36
- [H+]

H2O + Urea -> 2 NH3 + CO2		 (( (( (( (( (Vmax * K1) ) * K2) ) * H) ) + (( (Vmax1 * K1) ) * (H ^ 2))) ) / (( (( (K1 + H) ) * (K2 + H)) ) * (K3 + H))) buffer: 25 mM HEPES, 0.5 mM EDTA
PH: 7.75
Temperature: 36
- [H+]

Urea + H2O -> 2 NH3 + CO2		 (( (( (Vmax * K1) ) * H) ) / (( (K1 + H) ) * (K2 + H))) buffer: 50 mM HEPES
PH: 7.75
Temperature: 36
- [H+]

Urea + H2O -> 2 NH3 + CO2		 (( (( (Vmax * K1) ) * H) ) / (( (K1 + H) ) * (K2 + H))) buffer: 50 mM MES
PH: 5.1
Temperature: 36

Pathways

pathway id name
BioCyc:MTBH37RV_PWY-5704 urea degradation II
BioCyc:MTBCDC1551_PWY-5704 urea degradation II
BioCyc:AGRO_PWY-5704 urea degradation II
BioCyc:BSUB_PWY-5704 urea degradation II
BioCyc:GCF_000013425_PWY-5704 urea degradation II
BioCyc:ARA_PWY-5704 urea degradation II
BioCyc:META_PWY-5704 urea degradation II
BioCyc:HPY_PWY-5704 urea degradation II
BioCyc:CORYNE_PWY-5704 urea degradation II
PlantCyc:PLANT_URDEGR-PWY superpathway of allantoin degradation in plants
PlantCyc:PLANT_PWY-5704 urea degradation II
PlantCyc:ARA_URDEGR-PWY superpathway of allantoin degradation in plants
PlantCyc:ARA_PWY-5704 urea degradation II