uniprot:P77718 [tRNA sulfurtransferase(Enzyme) wildtype N-terminal His6-tag]
ATP + tRNA -> Diphosphate + adenylated_tRNA_(U8) |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris, 1 mM MgCl2, 0.1 mM sodium azid, 0.2 M 2‐amino‐6‐mercapto‐7‐methylpurine ribonucleoside (MESG), 1 U purine nucleoside phosphorylase, 0.03 U inorganic pyrophosphatase
PH: 7.5
Temperature: 22 |
uniprot:P77718 [tRNA sulfurtransferase(Enzyme) wildtype N-terminal His6-tag]
GTP + tRNA -> Diphosphate + Guanylated_tRNA_(U8) |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris, 1 mM MgCl2, 0.1 M sodium azid, 0.2 M 2‐amino‐6‐mercapto‐7‐methylpurine ribonucleoside (MESG), 1 U purine nucleoside phosphorylase, 0.03 U inorganic pyrophosphatase
PH: 7.5
Temperature: 22 |