EC: 2.7.7.69

GDP-L-galactose/GDP-D-glucose: hexose 1-phosphate guanylyltransferase (GDP-beta-L-galactose/GDP-alpha-D-glucose:hexose 1-phosphate guanylyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q8RWE8 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC2 His-tagged]

GDP + D_Mannose_1_phosphate -> Phosphate + GDP_D_mannose		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM NaCl, 1 mM Dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q8RWE8 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC2 His-tagged]

Phosphate + GDP_D_mannose -> GDP + D_Mannose_1_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2 mM MgCl2, 10 mM NaCl, 1 mM Dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q8RWE8 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC2 His-tagged]

GDP + L_Galactose_1_phosphate -> Phosphate + GDP_L_galactose		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM NaCl, 1 mM Dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q8RWE8 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC2 His-tagged]

Phosphate + GDP_D_glucose -> GDP + D_Glucose_1_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2 mM MgCl2, 10 mM NaCl, 1 mM Dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q8RWE8 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC2 His-tagged]

D_Glucose_1_phosphate + GDP -> GDP_D_glucose + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM NaCl, 1 mM Dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q8RWE8 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC2 His-tagged]

D_Galactose_1_phosphate + GDP -> Phosphate + GDP_D_galactose		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM NaCl, 1 mM Dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q8RWE8 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC2 His-tagged]

Phosphate + GDP_L_galactose -> GDP + L_Galactose_1_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2 mM MgCl2, 10 mM NaCl, 1 mM dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q9FLP9 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC5 His-tagged]

Phosphate + GDP_L_galactose -> GDP + L_Galactose_1_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2 mM MgCl2, 10 mM NaCl, 1 mM dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q9FLP9 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC5 His-tagged]

Phosphate + GDP_L_galactose -> L_Galactose_1_phosphate + GDP		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 2 mM MgCl2, 10 mM NaCl, 1 mM dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q8RWE8 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC2 His-tagged]

Phosphate + GDP_L_galactose -> GDP + L_Galactose_1_phosphate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 2 mM MgCl2, 10 mM NaCl, 1 mM dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q9FLP9 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC5 His-tagged]

GDP_D_glucose + Phosphate -> D_Glucose_1_phosphate + GDP		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 2 mM MgCl2, 10 mM NaCl, 1 mM dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q8RWE8 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC2 His-tagged]

Phosphate + GDP_D_glucose -> D_Glucose_1_phosphate + GDP		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 2 mM MgCl2, 10 mM NaCl, 1 mM dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q8RWE8 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC2 His-tagged]

GDP_L_galactose + Phosphate -> GDP + L_Galactose_1_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2 mM MgCl2, 10 mM NaCl, 1 mM Dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q9FLP9 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC5 His-tagged]

GDP_D_mannose + Phosphate -> D_Mannose_1_phosphate + GDP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2 mM MgCl2, 10 mM NaCl, 1 mM dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q9FLP9 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC5 His-tagged]

L_Galactose_1_phosphate + GDP -> Phosphate + GDP_L_galactose		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM NaCl, 1 mM dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q9FLP9 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC5 His-tagged]

GDP + D_Glucose_1_phosphate -> GDP_D_glucose + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM NaCl, 1 mM dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q9FLP9 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC5 His-tagged]

GDP_D_glucose + Phosphate -> GDP + D_Glucose_1_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 2 mM MgCl2, 10 mM NaCl, 1 mM dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q8RWE8 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC2 His-tagged]

GDP_L_galactose + Phosphate -> GDP + L_Galactose_1_phosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 2 mM MgCl2, 10 mM NaCl, 1 mM dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q9FLP9 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC5 His-tagged]

GDP_L_galactose + Phosphate -> GDP + L_Galactose_1_phosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 2 mM MgCl2, 10 mM NaCl, 1 mM dithiothreitol
PH: 7.5
Temperature: 26
- [GDP-L-galactose phosphorylase(Enzyme) wildtype]

Phosphate + GDP_L_galactose -> L_Galactose_1_phosphate + GDP		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 2 mM MgCl2, 10 mM NaCl, 1 mM dithiothreitol
PH: 7.5
Temperature: 26
- [GDP-L-galactose phosphorylase(Enzyme) wildtype]

GDP_L_galactose + Phosphate -> GDP + L_Galactose_1_phosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 2 mM MgCl2, 10 mM NaCl, 1 mM dithiothreitol
PH: 7.5
Temperature: 26
uniprot:Q9FLP9 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC5 his-tagged]

GDP_L_galactose + Phosphate -> GDP + L_Galactose_1_phosphate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM HEPES, 50 mM KCl, 10 mM MgCl2, 2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 U/ml lactate dehydrogenase, 1 U/ml pyruvate kinase, 5 mM K2HPO4
PH: 6.9
Temperature: 20
uniprot:Q8RWE8 [GDP-L-galactose phosphorylase(Enzyme) mutant Ser290Phe VTC2-3 his-tagged]

GDP_L_galactose + Phosphate -> GDP + L_Galactose_1_phosphate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM HEPES, 50 mM KCl, 10 mM MgCl2, 2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 U/ml lactate dehydrogenase, 1 U/ml pyruvate kinase, 5 mM K2HPO4
PH: 6.9
Temperature: 20
uniprot:Q8RWE8 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC2 his-tagged]

GDP_L_galactose + Phosphate -> GDP + L_Galactose_1_phosphate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM HEPES, 50 mM KCl, 10 mM MgCl2, 2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 U/ml lactate dehydrogenase, 1 U/ml pyruvate kinase, 5 mM K2HPO4
PH: 6.9
Temperature: 20
uniprot:Q9FLP9 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC5 his-tagged]

GDP_L_galactose + Phosphate -> GDP + L_Galactose_1_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 50 mM KCl, 10 mM MgCl2, 2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 U/ml lactate dehydrogenase, 1 U/ml pyruvate kinase, 5 mM K2HPO4
PH: 6.9
Temperature: 20
uniprot:Q8RWE8 [GDP-L-galactose phosphorylase(Enzyme) wildtype VTC2 his-tagged]

GDP_L_galactose + Phosphate -> GDP + L_Galactose_1_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 50 mM KCl, 10 mM MgCl2, 2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 U/ml lactate dehydrogenase, 1 U/ml pyruvate kinase, 5 mM K2HPO4
PH: 6.9
Temperature: 20
uniprot:Q8RWE8 [GDP-L-galactose phosphorylase(Enzyme) mutant Ser290Phe VTC2-3 his-tagged]

GDP_L_galactose + Phosphate -> L_Galactose_1_phosphate + GDP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 50 mM KCl, 10 mM MgCl2, 2 mM phosphoenolpyruvate, 0.2 mM NADH, 2 U/ml lactate dehydrogenase, 1 U/ml pyruvate kinase, 5 mM K2HPO4
PH: 6.9
Temperature: 20
- [GDP-L-galactose phosphorylase(Enzyme) wildtype]

Phosphate + GDP_L_galactose -> L_Galactose_1_phosphate + GDP		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris, 3 mM MgCl2
PH: 7.5
Temperature: 20
- [GDP-L-galactose phosphorylase(Enzyme) wildtype]

Phosphate + GDP_L_galactose -> GDP + L_Galactose_1_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris, 3 mM MgCl2
PH: 7.5
Temperature: 20

Pathways

pathway id name
BioCyc:META_PWY4FS-12 VTC2 cycle
BioCyc:META_PWY-882 L-ascorbate biosynthesis I (L-galactose pathway)
BioCyc:META_PWY4FS-13 extended VTC2 cycle
PathBank:SMP0012446 Ascorbate Metabolism