EC: 2.7.1.76

2'-deoxyadenosine kinase (ATP:2'-deoxyadenosine 5'-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q54YL2 [deoxyadenosine kinase(Enzyme) wildtype]

ATP + Fludarabine -> ADP + Fludarabine_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM MgCl2, 10 mM Dithiothreitol, 3 mg/ml BSA
PH: 8
Temperature: 22
uniprot:Q54YL2 [deoxyadenosine kinase(Enzyme) wildtype]

Thymidine + ATP -> dTMP + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM MgCl2, 10 mM Dithiothreitol, 3 mg/ml BSA
PH: 8
Temperature: 22
uniprot:Q54YL2 [deoxyadenosine kinase(Enzyme) wildtype]

Deoxyadenosine + ATP -> ADP + dAMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM MgCl2, 10 mM Dithiothreitol, 3 mg/ml BSA
PH: 8
Temperature: 22
uniprot:Q93IG4 [Mg2+]

Deoxyadenosine + ATP -> ADP + dAMP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.01 M HEPES, 0.15 M NaCl
PH: 7.4
Temperature: 22
uniprot:Q93IG4 [Mg2+]

ATP + Deoxyadenosine -> ADP + dAMP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.01 M HEPES, 0.15 M NaCl
PH: 7.4
Temperature: 22
uniprot:Q93IG4 [Mg2+]

UTP + Deoxyadenosine -> UDP + dAMP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.01 M HEPES, 0.15 M NaCl
PH: 7.4
Temperature: 22
uniprot:Q93IG4 [Mg2+]

Deoxyadenosine + CTP -> dAMP + CDP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.01 M HEPES, 0.15 M NaCl
PH: 7.4
Temperature: 22
uniprot:Q93IG4 [Mg2+]

CTP + Deoxyadenosine -> CDP + dAMP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.01 M HEPES, 0.15 M NaCl
PH: 7.4
Temperature: 22
uniprot:Q93IG4 [Mg2+]

dTTP + Deoxyadenosine -> TDP + dAMP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.01 M HEPES, 0.15 M NaCl
PH: 7.4
Temperature: 22
uniprot:Q93IG4 [Mg2+]

ATP + Deoxyadenosine -> dAMP + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Hepes/KOH, 0.5 mg/ml BSA
PH: 7.4
Temperature: 37
uniprot:Q93IG4 [Mg2+]

Deoxyadenosine + ATP -> dAMP + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Hepes/KOH, 0.5 mg/ml BSA
PH: 7.4
Temperature: 37
uniprot:Q93IG4 [Mg2+]

UTP + Deoxyadenosine -> dAMP + UDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Hepes/KOH, 0.5 mg/ml BSA
PH: 7.4
Temperature: 37
uniprot:Q93IG4 [Mg2+]

CTP + Deoxyadenosine -> CDP + dAMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Hepes/KOH, 0.5 mg/ml BSA
PH: 7.4
Temperature: 37
uniprot:Q93IG4 [Mg2+]

Deoxyadenosine + dTTP -> dAMP + TDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Hepes/KOH, 0.5 mg/ml BSA
PH: 7.4
Temperature: 37
uniprot:Q7M0X4 [2'-Deoxyadenonosine 5'-adenosine 5'''-P1,P4-tetraphosphate]

ATP + Deoxyadenosine -> dAMP + ADP		 (( (Vmax * A) ) / (( (Ka * (1 + (I / Ki))) ) + (A * (1 + (I / Ki))))) buffer: 0.1 M Tris-HCl, 0.36 mM MgCl2
PH: 8
Temperature: 20
uniprot:Q7M0X4 [dATP]

Deoxyadenosine + ATP -> dAMP + ADP		 (Vmax / (( (( (( (Ka * (1 + (( (( (Kia * Kb) ) / Ka) ) * B))) ) * (1 + (I / Ki))) ) / A) ) + (1 + (Kb / B)))) buffer: 0.1 M Tris-HCl, 0.36 mM MgCl2
PH: 8
Temperature: 20
uniprot:Q7M0X4 [2'-Deoxyadenonosine 5'-adenosine 5'''-P1,P4-tetraphosphate]

ATP + Deoxyadenosine -> dAMP + ADP		 (Vmax / (( (( (( (Ka * (1 + (( (( (Kia * Kb) ) / Ka) ) * B))) ) * (1 + (I / Ki))) ) / A) ) + (1 + (Kb / B)))) buffer: 0.1 M Tris-HCl, 0.36 mM MgCl2
PH: 8
Temperature: 20
- [2'-Deoxyadenonosine 5'-adenosine 5'''-P1,P4-tetraphosphate]

Deoxyadenosine + ATP -> dAMP + ADP		 (Vmax / (( (( (( (Ka * (1 + (( (( (Kia * Kb) ) / Ka) ) * B))) ) * (1 + (I / Ki))) ) / A) ) + (1 + (Kb / B)))) buffer: 0.1 M Tris-HCl, 0.36 mM MgCl2
PH: 8
Temperature: 20
uniprot:Q7M0X4 [2'-Deoxyadenonosine 5'-adenosine 5'''-P1,P4-tetraphosphate]

Deoxyadenosine + ATP -> dAMP + ADP		 (( (Vmax * A) ) / (( (Ka * (1 + (I / Ki))) ) + A)) buffer: 0.1 M Tris-HCl, 0.36 mM MgCl2
PH: 8
Temperature: 20
- [2'-Deoxyadenonosine 5'-adenosine 5'''-P1,P4-tetraphosphate]

Deoxyadenosine + ATP -> dAMP + ADP		 (( (Vmax * A) ) / (( (Ka * (1 + (I / Ki))) ) + (A * (1 + (I / Ki))))) buffer: 0.1 M Tris-HCl, 0.36 mM MgCl2
PH: 8
Temperature: 20
- [2'-Deoxyadenonosine 5'-adenosine 5'''-P1,P4-tetraphosphate]

ATP + Deoxyadenosine -> dAMP + ADP		 (( (Vmax * A) ) / (( (Ka * (1 + (I / Ki))) ) + A)) buffer: 0.1 M Tris-HCl, 0.36 mM MgCl2
PH: 8
Temperature: 20
- [dATP]

Deoxyadenosine + ATP -> dAMP + ADP		 (Vmax / (( (( (( (Ka * (1 + (( (( (Kia * Kb) ) / Ka) ) * B))) ) * (1 + (I / Ki))) ) / A) ) + (1 + (Kb / B)))) buffer: 0.1 M Tris-HCl, 0.36 mM MgCl2
PH: 8
Temperature: 20
uniprot:P37529 [deoxyadenosine kinase(Enzyme) wildtype]

Deoxyadenosine + GTP -> dAMP + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2 mM mercaptoethanol, 8% glycerol, 0.5 mM MgCl
PH: 8.5
Temperature: 37
uniprot:P37529 [deoxyadenosine kinase(Enzyme) wildtype]

GTP + Deoxyadenosine -> dAMP + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2 mM mercaptoethanol, 8% glycerol, 0.5 mM MgCl
PH: 8.5
Temperature: 37
uniprot:P37529 [deoxyadenosine kinase(Enzyme) wildtype]

Deoxyadenosine + GTP -> dAMP + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2 mM mercaptoethanol, 8% glycerol, 0.5 mM MgCl
PH: 8.5
Temperature: 37
uniprot:P37529 [deoxyadenosine kinase(Enzyme) wildtype]

Deoxyadenosine + GTP -> GDP + dAMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2 mM mercaptoethanol, 8% glycerol, 0.5 mM MgCl
PH: 8.5
Temperature: 37
uniprot:P37529 [deoxyadenosine kinase(Enzyme) wildtype]

GTP + Deoxyadenosine -> GDP + dAMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2 mM mercaptoethanol, 8% glycerol, 0.5 mM MgCl
PH: 8.5
Temperature: 37
uniprot:P37529 [deoxyadenosine kinase(Enzyme) wildtype]

GTP + Deoxyadenosine -> dAMP + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2 mM mercaptoethanol, 8% glycerol, 0.5 mM MgCl
PH: 8.5
Temperature: 37
uniprot:Q0H0H0 [deoxyadenosine kinase(Enzyme) wildtype]

2'_Deoxycytidine + ATP -> ADP + dCMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM DTT, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml BSA, 0.5 mM CHAPS
PH: 8
Temperature: 36
uniprot:Q0H0H0 [deoxyadenosine kinase(Enzyme) wildtype]

Deoxyadenosine + ATP -> dAMP + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM DTT, 2.5 mM MgCl2, 3 mg/ml BSA, 0.5 mM CHAPS
PH: 8
Temperature: 36
uniprot:Q0H0H0 [deoxyadenosine kinase(Enzyme) wildtype]

Gemcitabine + ATP -> Gemcitabine_monophosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM DTT, 2.5 mM MgCl2, 3 mg/ml BSA, 0.5 mM CHAPS
PH: 8
Temperature: 36
- [deoxyadenosine kinase(Enzyme) wildtype]

Deoxyadenosine + ATP -> dAMP + ADP		 (( (Vmax * S1) ) / (Km + S1)) buffer: 40 mM Tris-HCl, 10 mM MgCl2, 0.25 mg/ml BSA, 12.5 mM Dithiothreitol
PH: 8
Temperature: 37
- [deoxyadenosine kinase(Enzyme) wildtype]

ATP + Deoxyadenosine -> dAMP + ADP		 (( (Vmax * S1) ) / (Km + S1)) buffer: 40 mM Tris-HCl, 10 mM MgCl2, 0.25 mg/ml BSA, 12.5 mM Dithiothreitol
PH: 8
Temperature: 37
- [deoxyadenosine kinase(Enzyme) wildtype]

ATP + Deoxyadenosine -> ADP + dAMP		 (( (Vmax * S1) ) / (Km + S1)) buffer: 40 mM Tris-HCl, 10 mM MgCl2, 0.25 mg/ml BSA, 12.5 mM Dithiothreitol
PH: 8
Temperature: 37
- [deoxyadenosine kinase(Enzyme) wildtype]

Deoxyadenosine + ATP -> ADP + dAMP		 (( (Vmax * S1) ) / (Km + S1)) buffer: 40 mM Tris-HCl, 10 mM MgCl2, 0.25 mg/ml BSA, 12.5 mM Dithiothreitol
PH: 8
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-7224 purine deoxyribonucleosides salvage
BioCyc:HUMAN_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:META_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:META_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:META_PWY-7224 purine deoxyribonucleosides salvage
BioCyc:HUMAN_PWY-7199 pyrimidine deoxyribonucleosides salvage
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-73614 Pyrimidine salvage
Reactome:R-DDI-8956321 Nucleotide salvage
Reactome:R-HSA-74217 Purine salvage
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-74217 Purine salvage
Reactome:R-RNO-74217 Purine salvage
Reactome:R-BTA-74217 Purine salvage
Reactome:R-DDI-15869 Nucleotide metabolism
Reactome:R-HSA-8956321 Nucleotide salvage
Reactome:R-MMU-8956321 Nucleotide salvage
Reactome:R-RNO-8956321 Nucleotide salvage
Reactome:R-BTA-15869 Nucleotide metabolism
Reactome:R-DDI-74217 Purine salvage
Reactome:R-HSA-73614 Pyrimidine salvage
Reactome:R-MMU-73614 Pyrimidine salvage
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-73614 Pyrimidine salvage
Reactome:R-BTA-8956321 Nucleotide salvage
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-73614 Pyrimidine salvage
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-RNO-15869 Nucleotide metabolism
WikiPathways:WP4792 Purine metabolism
WikiPathways:WP4224 Purine metabolism and related disorders
PathBank:SMP0120670 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000167 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000220 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0000512 Xanthinuria Type I
PathBank:SMP0000536 Mitochondrial DNA Depletion Syndrome-3
PathBank:SMP0000649 Lamivudine Metabolism Pathway
PathBank:SMP0120669 Adenosine Deaminase Deficiency
PathBank:SMP0120673 AICA-Ribosiduria
PathBank:SMP0120709 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0120717 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0120797 Xanthinuria Type II
PathBank:SMP0120821 Myoadenylate Deaminase Deficiency
PathBank:SMP0000050 Purine Metabolism
PathBank:SMP0000144 Adenosine Deaminase Deficiency
PathBank:SMP0000203 Molybdenum Cofactor Deficiency
PathBank:SMP0000446 Gemcitabine Action Pathway
PathBank:SMP0000428 Mercaptopurine Action Pathway
PathBank:SMP0000535 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0120728 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0120752 Molybdenum Cofactor Deficiency
PathBank:SMP0120756 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0120796 Xanthinuria Type I
PathBank:SMP0120820 Mitochondrial DNA Depletion Syndrome
PathBank:SMP0000365 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0000210 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0000430 Thioguanine Action Pathway
PathBank:SMP0000513 Xanthinuria Type II
PathBank:SMP0000537 Myoadenylate Deaminase Deficiency
PathBank:SMP0087332 Purine Metabolism
PathBank:SMP0120819 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0000364 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0000168 AICA-Ribosiduria
PathBank:SMP0000427 Azathioprine Action Pathway
PathBank:SMP0000603 Gemcitabine Metabolism Pathway
PathBank:SMP0087239 Purine Metabolism