EC: 2.7.1.74

deoxycytidine kinase (NTP:deoxycytidine 5'-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

Deoxyguanosine + ATP -> ADP + dGMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Imidazole, 25 mM Dithiothreitol, 2.5 mM MgCl2, 5 % Glycerol, 1 mg/ml BSA
PH: 7.4
Temperature: 37
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

D_2'_Deoxycarbocyclic_guanosine + ATP -> ADP + D_2'_Deoxycarbocyclic_guanosine_5'_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Imidazole, 25 mM Dithiothreitol, 2.5 mM MgCl2, 5 % Glycerol, 1 mg/ml BSA
PH: 7.4
Temperature: 37
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

L_2'_Deoxycarbocyclic_guanosine + ATP -> ADP + L_2'_Deoxycarbocyclic_guanosine_5'_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Imidazole, 25 mM Dithiothreitol, 2.5 mM MgCl2, 5 % Glycerol, 1 mg/ml BSA
PH: 7.4
Temperature: 37
uniprot:Q93IG4 [Mg2+]

2'_Deoxycytidine + ATP -> dCMP + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.01 M HEPES, 0.15 M NaCl
PH: 7.4
Temperature: 22
uniprot:Q93IG4 [Mg2+]

CTP + 2'_Deoxycytidine -> CDP + dCMP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.01 M HEPES, 0.15 M NaCl
PH: 7.4
Temperature: 22
uniprot:Q93IG4 [Mg2+]

2'_Deoxycytidine + ATP -> ADP + dCMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Hepes/KOH, 0.5 mg/ml BSA
PH: 7.4
Temperature: 37
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

2'_Deoxycytidine + ATP -> dCMP + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

Deoxyguanosine + ATP -> dGMP + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 37
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

Deoxyadenosine + ATP -> ADP + dAMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 µM EHNA
PH: 8
Temperature: 37
uniprot:Q7M0X4 [2'-Deoxycytidine 5'-adenosine 5'''-P1,P4-tetraphosphate]

ATP + 2'_Deoxycytidine -> ADP + dCMP		 (( (Vmax * A) ) / (( (Ka * (1 + (I / Ki))) ) + A)) buffer: 0.1 M Tris-HCl, 0.36 mM MgCl2
PH: 8
Temperature: 20
uniprot:Q7M0X4 [2'-Deoxycytidine 5'-adenosine 5'''-P1,P4-tetraphosphate]

2'_Deoxycytidine + ATP -> ADP + dCMP		 (( (Vmax * A) ) / (( (Ka * (1 + (I / Ki))) ) + A)) buffer: 0.1 M Tris-HCl, 0.36 mM MgCl2
PH: 8
Temperature: 20
uniprot:Q7M0X4 [dCTP]

ATP + 2'_Deoxycytidine -> dCMP + ADP		 (( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (Ka * B)) ) + (Kb * A)) ) + (( (Kia * Kb) ) * (1 + (I / Ki))))) buffer: 0.1 M Tris-HCl, 0.36 mM MgCl2
PH: 8
Temperature: 20
uniprot:Q7M0X4 [2'-Deoxycytidine 5'-adenosine 5'''-P1,P4-tetraphosphate]

2'_Deoxycytidine + ATP -> ADP + dCMP		 (( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (Ka * B)) ) + (Kb * A)) ) + (( (Kia * Kb) ) * (1 + (I / Ki))))) buffer: 0.1 M Tris-HCl, 0.36 mM MgCl2
PH: 8
Temperature: 20
uniprot:P37529 [deoxycytidine kinase(Enzyme) wildtype]

GTP + 2'_Deoxycytidine -> dCMP + GDP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2 mM mercaptoethanol, 8% glycerol, 0.5 mM MgCl
PH: 8.5
Temperature: 37
uniprot:P37529 [deoxycytidine kinase(Enzyme) wildtype]

GTP + 2'_Deoxycytidine -> GDP + dCMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2 mM mercaptoethanol, 8% glycerol, 0.5 mM MgCl
PH: 8.5
Temperature: 37
uniprot:P48769 [deoxycytidine kinase(Enzyme) wildtype]

ATP + 2'_Deoxycytidine -> ADP + dCMP		 (( (Vmax * S1) ) / (Km + S1)) buffer: 0.12 M Tris-HCl, 10 mM MgCl2, 1 % BSA
PH: 7.8
Temperature: 37
uniprot:P48769 [deoxycytidine kinase(Enzyme) wildtype]

ATP + 2'_Deoxycytidine -> ADP + dCMP		 (( (Vmax * S1) ) / (Km + S1)) buffer: 0.12 M Tris-HCl, 10 mM MgCl2, 1 % BSA
PH: 7.8
Temperature: 37
uniprot:P48769 [deoxycytidine kinase(Enzyme) wildtype]

2'_Deoxycytidine + ATP -> dCMP + ADP		 (( (Vmax * S1) ) / (Km + S1)) buffer: 0.12 M Tris-HCl, 10 mM MgCl2, 1 % BSA
PH: 7.8
Temperature: 37
uniprot:P48769 [deoxycytidine kinase(Enzyme) wildtype]

2'_Deoxycytidine + ATP -> dCMP + ADP		 (( (Vmax * S1) ) / (Km + S1)) buffer: 0.12 M Tris-HCl, 10 mM MgCl2, 1 % BSA
PH: 7.8
Temperature: 37
uniprot:O00142 [deoxycytidine kinase(Enzyme) wildtype mitochondrial dCyd kinase]

Cytarabine + ATP -> 1_beta_D_Arabinofuranosylcytosine_5'_monophosphate + ADP		 (( (V * B) ) / (Km + B)) buffer: 0.15 mM Tris-HCl, 5 mM MgCl2, 2 mM dithiothreitol, 1 mg/ml BSA
PH: 7.5
Temperature: 37
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

ATP + 2'_Deoxycytidine -> ADP + dCMP		 (( (Vmax * A) ) / (Km + A)) buffer: 10 µM Tetrahydrouridine, 2.5 mM MgCl2, 50 mM Tris-HCl, 100 mM KCl, 15 mM NaF, 2.5 mM DTT
PH: 7.5
Temperature: 36
uniprot:Q4R5R5 [deoxycytidine kinase(Enzyme) wildtype]

ATP + 2'_Deoxycytidine -> ADP + dCMP		 (( (Vmax * A) ) / (Km + A)) buffer: 10 µM Tetrahydrouridine, 2.5 mM MgCl2, 50 mM Tris-HCl, 100 mM KCl, 15 mM NaF, 2.5 mM DTT
PH: 7.5
Temperature: 36
uniprot:P43346 [deoxycytidine kinase(Enzyme) wildtype]

2'_Deoxycytidine + ATP -> ADP + dCMP		 (( (Vmax * A) ) / (Km + A)) buffer: 10 µM Tetrahydrouridine, 2.5 mM MgCl2, 50 mM Tris-HCl, 100 mM KCl, 15 mM NaF, 2.5 mM DTT
PH: 7.5
Temperature: 36
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

Deoxyadenosine + ATP -> dAMP + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 50 µM Erythro-9-(2-hydroxy-3-nonyl)adenine, 2.5 mM MgCl2, 50 mM Tris-HCl, 100 mM KCl, 15 mM NaF, 2.5 mM DTT
PH: 7.5
Temperature: 36
uniprot:P43346 [deoxycytidine kinase(Enzyme) wildtype]

ATP + Deoxyadenosine -> dAMP + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 50 µM Erythro-9-(2-hydroxy-3-nonyl)adenine, 2.5 mM MgCl2, 50 mM Tris-HCl, 100 mM KCl, 15 mM NaF, 2.5 mM DTT
PH: 7.5
Temperature: 36
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

ATP + Cytarabine -> 1_beta_D_Arabinofuranosylcytosine_5'_monophosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 10 µM Tetrahydrouridine, 2.5 mM MgCl2, 50 mM Tris-HCl, 100 mM KCl, 15 mM NaF, 2.5 mM DTT
PH: 7.5
Temperature: 36
uniprot:P43346 [deoxycytidine kinase(Enzyme) wildtype]

ATP + Cytarabine -> 1_beta_D_Arabinofuranosylcytosine_5'_monophosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 10 µM Tetrahydrouridine, 2.5 mM MgCl2, 50 mM Tris-HCl, 100 mM KCl, 15 mM NaF, 2.5 mM DTT
PH: 7.5
Temperature: 36
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

ATP + Zalcitabine -> L_ddCMP + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 10 µM Tetrahydrouridine, 2.5 mM MgCl2, 50 mM Tris-HCl, 100 mM KCl, 15 mM NaF, 2.5 mM DTT
PH: 7.5
Temperature: 36
uniprot:P43346 [deoxycytidine kinase(Enzyme) wildtype]

Zalcitabine + ATP -> ADP + L_ddCMP		 (( (Vmax * A) ) / (Km + A)) buffer: 10 µM Tetrahydrouridine, 2.5 mM MgCl2, 50 mM Tris-HCl, 100 mM KCl, 15 mM NaF, 2.5 mM DTT
PH: 7.5
Temperature: 36
uniprot:Q4R5R5 [deoxycytidine kinase(Enzyme) wildtype]

Deoxyadenosine + ATP -> ADP + dAMP		 (( (Vmax * A) ) / (Km + A)) buffer: 50 µM Erythro-9-(2-hydroxy-3-nonyl)adenine, 2.5 mM MgCl2, 50 mM Tris-HCl, 100 mM KCl, 15 mM NaF, 2.5 mM DTT
PH: 7.5
Temperature: 36
uniprot:Q4R5R5 [deoxycytidine kinase(Enzyme) wildtype]

Cytarabine + ATP -> ADP + 1_beta_D_Arabinofuranosylcytosine_5'_monophosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 10 µM Tetrahydrouridine, 2.5 mM MgCl2, 50 mM Tris-HCl, 100 mM KCl, 15 mM NaF, 2.5 mM DTT
PH: 7.5
Temperature: 36
uniprot:Q4R5R5 [deoxycytidine kinase(Enzyme) wildtype]

ATP + Zalcitabine -> ADP + L_ddCMP		 (( (Vmax * A) ) / (Km + A)) buffer: 10 µM Tetrahydrouridine, 2.5 mM MgCl2, 50 mM Tris-HCl, 100 mM KCl, 15 mM NaF, 2.5 mM DTT
PH: 7.5
Temperature: 36
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype cytosolic isoenzyme dCyd kinase I]

ATP + 2'_Deoxycytidine -> dCMP + ADP		 (( (V * B) ) / (Km + B)) buffer: 0.15 mM Tris-HCl, 5 mM MgCl2, 2 mM dithiothreitol, 1 mg/ml BSA
PH: 7.5
Temperature: 37
uniprot:O00142 [deoxycytidine kinase(Enzyme) wildtype mitochondrial dCyd kinase]

2'_Deoxycytidine + ATP -> dCMP + ADP		 (( (V * B) ) / (Km + B)) buffer: 0.15 mM Tris-HCl, 5 mM MgCl2, 2 mM dithiothreitol, 1 mg/ml BSA
PH: 7.5
Temperature: 37
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype cytosolic isoenzyme dCyd kinase I]

Cytarabine + ATP -> ADP + 1_beta_D_Arabinofuranosylcytosine_5'_monophosphate		 (( (V * B) ) / (Km + B)) buffer: 0.15 mM Tris-HCl, 5 mM MgCl2, 2 mM dithiothreitol, 1 mg/ml BSA
PH: 7.5
Temperature: 37
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

2'_Deoxycytidine + ATP -> dCMP + ADP		 (( (( (Vmax1_app * A) ) / (Km1_app + A)) ) + (( (Vmax2_app * A) ) / (Km2_app + A))) buffer: 0.1 M HEPES, 3 mM DTT, 10 mM MgCl2, 0.5 mg/ml BSA
PH: 7.5
Temperature: 37
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

ATP + Deoxyguanosine -> dGMP + ADP		 (( (( (Vmax1_app * A) ) / (Km1_app + A)) ) + (( (Vmax2_app * A) ) / (Km2_app + A))) buffer: 0.1 M HEPES, 3 mM DTT, 10 mM MgCl2, 0.5 mg/ml BSA
PH: 7.5
Temperature: 37
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

Deoxyadenosine + ATP -> ADP + dAMP		 (( (( (Vmax1_app * A) ) / (Km1_app + A)) ) + (( (Vmax2_app * A) ) / (Km2_app + A))) buffer: 0.1 M HEPES, 3 mM DTT, 10 mM MgCl2, 0.5 mg/ml BSA
PH: 7.5
Temperature: 37
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

Vidarabine + ATP -> ADP + 9_beta_D_Arabinofuranosyladenine_5'_monophosphate		 (( (Vmax_app * A) ) / (Km_app + A)) buffer: 0.1 M HEPES, 3 mM DTT, 10 mM MgCl2, 0.5 mg/ml BSA
PH: 7.5
Temperature: 37
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

ATP + 2'_Deoxycytidine -> dCMP + ADP		 (( (( (Vmax1_app * A) ) / (Km1_app + A)) ) + (( (Vmax2_app * A) ) / (Km2_app + A))) buffer: 0.1 M HEPES, 3 mM DTT, 10 mM MgCl2, 0.5 mg/ml BSA
PH: 7.5
Temperature: 37
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

ATP + Deoxyguanosine -> ADP + dGMP		 (( (( (Vmax1_app * A) ) / (Km1_app + A)) ) + (( (Vmax2_app * A) ) / (Km2_app + A))) buffer: 0.1 M HEPES, 3 mM DTT, 10 mM MgCl2, 0.5 mg/ml BSA
PH: 7.5
Temperature: 37
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

ATP + Deoxyadenosine -> ADP + dAMP		 (( (( (Vmax1_app * A) ) / (Km1_app + A)) ) + (( (Vmax2_app * A) ) / (Km2_app + A))) buffer: 0.1 M HEPES, 3 mM DTT, 10 mM MgCl2, 0.5 mg/ml BSA
PH: 7.5
Temperature: 37
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

ATP + Cytarabine -> 1_beta_D_Arabinofuranosylcytosine_5'_monophosphate + ADP		 (( (( (Vmax1_app * A) ) / (Km1_app + A)) ) + (( (Vmax2_app * A) ) / (Km2_app + A))) buffer: 0.1 M HEPES, 3 mM DTT, 10 mM MgCl2, 0.5 mg/ml BSA
PH: 7.5
Temperature: 37
uniprot:P27707 [deoxycytidine kinase(Enzyme) wildtype]

ATP + Vidarabine -> ADP + 9_beta_D_Arabinofuranosyladenine_5'_monophosphate		 (( (Vmax_app * A) ) / (Km_app + A)) buffer: 0.1 M HEPES, 3 mM DTT, 10 mM MgCl2, 0.5 mg/ml BSA
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-7224 purine deoxyribonucleosides salvage
BioCyc:HUMAN_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:META_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:META_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:MOUSE_P1-PWY purine and pyrimidine metabolism
BioCyc:MOUSE_PWY0-181 salvage pathways of pyrimidine deoxyribonucleotides
BioCyc:META_PWY-7224 purine deoxyribonucleosides salvage
BioCyc:HUMAN_PWY-7199 pyrimidine deoxyribonucleosides salvage
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-73614 Pyrimidine salvage
Reactome:R-HSA-74217 Purine salvage
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-74217 Purine salvage
Reactome:R-RNO-74217 Purine salvage
Reactome:R-BTA-74217 Purine salvage
Reactome:R-HSA-8956321 Nucleotide salvage
Reactome:R-MMU-8956321 Nucleotide salvage
Reactome:R-RNO-8956321 Nucleotide salvage
Reactome:R-BTA-15869 Nucleotide metabolism
Reactome:R-HSA-73614 Pyrimidine salvage
Reactome:R-MMU-73614 Pyrimidine salvage
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-73614 Pyrimidine salvage
Reactome:R-BTA-8956321 Nucleotide salvage
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-RNO-15869 Nucleotide metabolism
WikiPathways:WP4584 Biomarkers for pyrimidine metabolism disorders
WikiPathways:WP4225 Pyrimidine metabolism and related diseases
PathBank:SMP0120670 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000649 Lamivudine Metabolism Pathway
PathBank:SMP0120669 Adenosine Deaminase Deficiency
PathBank:SMP0120673 AICA-Ribosiduria
PathBank:SMP0120709 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0120717 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0120797 Xanthinuria Type II
PathBank:SMP0120821 Myoadenylate Deaminase Deficiency
PathBank:SMP0000446 Gemcitabine Action Pathway
PathBank:SMP0120728 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0120752 Molybdenum Cofactor Deficiency
PathBank:SMP0120756 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0120796 Xanthinuria Type I
PathBank:SMP0120820 Mitochondrial DNA Depletion Syndrome
PathBank:SMP0087332 Purine Metabolism
PathBank:SMP0120819 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0000603 Gemcitabine Metabolism Pathway
PathBank:SMP0087239 Purine Metabolism