uniprot:P20966 [protein-Npi-phosphohistidine---D-fructose phosphotransferase(Enzyme) wildtype]
Protein_N_phosphohistidine + D_Fructose -> Protein_histidine + D_Fructose_1_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM potassium phosphate, 10 mM MgCl, 20 mM KF, 1 mM dithiothreitol
PH: 7.4
Temperature: 37 |
uniprot:P20966 [protein-Npi-phosphohistidine---D-fructose phosphotransferase(Enzyme) wildtype]
Protein_N_phosphohistidine + D_Fructose -> Protein_histidine + D_Fructose_1_phosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 50 mM potassium phosphate, 10 mM MgCl, 20 mM KF, 1 mM dithiothreitol
PH: 7.4
Temperature: 37 |
uniprot:P20966 [protein-Npi-phosphohistidine---D-fructose phosphotransferase(Enzyme) mutant FruBC (N-terminal truncation)]
Protein_N_phosphohistidine + D_Fructose -> D_Fructose_1_phosphate + Protein_histidine |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM potassium phosphate, 10 mM MgCl, 20 mM KF, 1 mM dithiothreitol
PH: 7.4
Temperature: 37 |
uniprot:P20966 [protein-Npi-phosphohistidine---D-fructose phosphotransferase(Enzyme) mutant FruBC (N-terminal truncation)]
Protein_N_phosphohistidine + D_Fructose -> D_Fructose_1_phosphate + Protein_histidine |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 50 mM potassium phosphate, 10 mM MgCl, 20 mM KF, 1 mM dithiothreitol
PH: 7.4
Temperature: 37 |