EC: 2.7.1.145

deoxynucleoside kinase (ATP:deoxynucleoside 5'-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype]

Thymidine + ATP -> ADP + dTMP
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.5 mM CHAPS, 2.5 mM MgCl2, 10 mM Dithiothreitol, 3 mg/ml Bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype]

2'_Deoxycytidine + ATP -> ADP + dCMP
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.5 mM CHAPS, 2.5 mM MgCl2, 10 mM Dithiothreitol, 3 mg/ml Bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype]

ATP + Thymidine -> ADP + dTMP
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.5 mM CHAPS, 2.5 mM MgCl2, 10 mM Dithiothreitol, 3 mg/ml Bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype]

Deoxyguanosine + ATP -> dGMP + ADP
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.5 mM CHAPS, 2.5 mM MgCl2, 10 mM Dithiothreitol, 3 mg/ml Bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) mutant dNKdeltaC30]

ATP + Thymidine -> ADP + dTMP
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.5 mM CHAPS, 2.5 mM MgCl2, 10 mM Dithiothreitol, 3 mg/ml Bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype]

ATP + Deoxyadenosine -> dAMP + ADP
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.5 mM CHAPS, 2.5 mM MgCl2, 10 mM Dithiothreitol, 3 mg/ml Bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype]

2'_Deoxycytidine + ATP -> dCMP + ADP
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.5 mM CHAPS, 2.5 mM MgCl2, 10 mM Dithiothreitol, 3 mg/ml Bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype]

ATP + Cytarabine -> 1_beta_D_Arabinofuranosylcytosine_5'_monophosphate + ADP
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.5 mM CHAPS, 2.5 mM MgCl2, 10 mM Dithiothreitol, 3 mg/ml Bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype]

Thymidine + CTP -> dTMP + CDP
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.5 mM CHAPS, 2.5 mM MgCl2, 10 mM Dithiothreitol, 3 mg/ml Bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype]

ATP + Thymidine -> dTMP + ADP
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.5 mM CHAPS, 2.5 mM MgCl2, 10 mM Dithiothreitol, 3 mg/ml Bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype]

ATP + 2'_Deoxycytidine -> ADP + dCMP
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.5 mM CHAPS, 2.5 mM MgCl2, 10 mM Dithiothreitol, 3 mg/ml Bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) mutant dNKdeltaC10]

ATP + Thymidine -> dTMP + ADP
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.5 mM CHAPS, 2.5 mM MgCl2, 10 mM Dithiothreitol, 3 mg/ml Bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) mutant dNKdeltaC20]

Thymidine + ATP -> ADP + dTMP
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.5 mM CHAPS, 2.5 mM MgCl2, 10 mM Dithiothreitol, 3 mg/ml Bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype]

ATP + Deoxyguanosine -> dGMP + ADP
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.5 mM CHAPS, 2.5 mM MgCl2, 10 mM Dithiothreitol, 3 mg/ml Bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype]

Deoxyadenosine + ATP -> ADP + dAMP
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.5 mM CHAPS, 2.5 mM MgCl2, 10 mM Dithiothreitol, 3 mg/ml Bovine serum albumin
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype GST fusion protein]

Thymidine + ATP -> dTMP + ADP
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml BSA
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype GST fusion protein]

Deoxyguanosine + ATP -> dGMP + ADP
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml BSA
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype GST fusion protein]

ATP + 2'_Deoxycytidine -> ADP + dCMP
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml BSA
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype GST fusion protein]

Deoxyadenosine + ATP -> dAMP + ADP
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml BSA
PH: 8
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype]

ATP + Thymidine -> dTMP + ADP
(( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris/HCl, 0.1 mg/ml BSA, 5 mM MgCl2, 5 mM dithiothreitol
PH: 7.6
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) mutant F114A]

ATP + Thymidine -> ADP + dTMP
(( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris/HCl, 0.1 mg/ml BSA, 5 mM MgCl2, 5 mM dithiothreitol
PH: 7.6
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) mutant Q81N]

ATP + Thymidine -> dTMP + ADP
(( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris/HCl, 0.1 mg/ml BSA, 5 mM MgCl2, 5 mM dithiothreitol
PH: 7.6
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) mutant F114Y]

Thymidine + ATP -> ADP + dTMP
(( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris/HCl, 0.1 mg/ml BSA, 5 mM MgCl2, 5 mM dithiothreitol
PH: 7.6
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) mutant Q81N]

ATP + Deoxyguanosine -> dGMP + ADP
(( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris/HCl, 0.1 mg/ml BSA, 5 mM MgCl2, 5 mM dithiothreitol
PH: 7.6
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype]

Deoxyguanosine + ATP -> ADP + dGMP
(( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris/HCl, 0.1 mg/ml BSA, 5 mM MgCl2, 5 mM dithiothreitol
PH: 7.6
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) wildtype]

ATP + Deoxyadenosine -> dAMP + ADP
(( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris/HCl, 0.1 mg/ml BSA, 5 mM MgCl2, 5 mM dithiothreitol
PH: 7.6
Temperature: 37
uniprot:Q9XZT6 [deoxynucleoside kinase(Enzyme) mutant Q81N]

ATP + Deoxyadenosine -> ADP + dAMP
(( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris/HCl, 0.1 mg/ml BSA, 5 mM MgCl2, 5 mM dithiothreitol
PH: 7.6
Temperature: 37
uniprot:Q9BKL3 [deoxynucleoside kinase(Enzyme) wildtype His-tagged]

ATP + Thymidine -> ADP + dTMP
(( (V * (B ^ h)) ) / (( (S05 ^ h) ) + (B ^ h))) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37
uniprot:Q8UVZ9 [dTTP]

ATP + Thymidine -> dTMP + ADP
(( (V * B) ) / (( (Km * (1 + (I / Ki))) ) + B)) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37
uniprot:Q8UVZ9 [dTTP]

ATP + Thymidine -> ADP + dTMP
(( (V * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37
uniprot:Q8UVZ9 [deoxynucleoside kinase(Enzyme) wildtype]

Thymidine + ATP -> dTMP + ADP
(( (V * (B ^ h)) ) / (( (S05 ^ h) ) + (B ^ h))) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37
uniprot:Q9BKL3 [deoxynucleoside kinase(Enzyme) wildtype His-tagged]

2'_Deoxycytidine + ATP -> dCMP + ADP
(( (V * (B ^ h)) ) / (( (S05 ^ h) ) + (B ^ h))) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37
uniprot:Q8UVZ9 [deoxynucleoside kinase(Enzyme) wildtype]

2'_Deoxycytidine + ATP -> dCMP + ADP
(( (V * B) ) / (Km + B)) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37
uniprot:Q9BKL3 [deoxynucleoside kinase(Enzyme) wildtype His-tagged]

Deoxyguanosine + ATP -> ADP + dGMP
(( (V * (B ^ h)) ) / (( (S05 ^ h) ) + (B ^ h))) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37
uniprot:Q9BKL3 [deoxynucleoside kinase(Enzyme) wildtype His-tagged]

ATP + Deoxyadenosine -> ADP + dAMP
(( (V * (B ^ h)) ) / (( (S05 ^ h) ) + (B ^ h))) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37
uniprot:Q8UVZ9 [deoxynucleoside kinase(Enzyme) wildtype]

ATP + Deoxyguanosine -> ADP + dGMP
(( (V * B) ) / (Km + B)) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37
uniprot:Q8UVZ9 [deoxynucleoside kinase(Enzyme) wildtype]

ATP + Deoxyadenosine -> dAMP + ADP
(( (V * B) ) / (Km + B)) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37
uniprot:Q8UVZ9 [dCTP]

ATP + Thymidine -> dTMP + ADP
(( (V * B) ) / (Km + (( (1 + (I / Ki)) ) * B))) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37
uniprot:Q8UVZ9 [dCTP]

ATP + Thymidine -> ADP + dTMP
(( (V * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37
uniprot:Q9XZT6 [dTTP]

Thymidine + ATP -> dTMP + ADP
(( (V * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37
uniprot:Q9BKL3 [dTTP]

ATP + Thymidine -> dTMP + ADP
(( (V * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37
uniprot:Q8UVZ9 [dTTP]

2'_Deoxycytidine + ATP -> ADP + dCMP
(( (V * B) ) / (Km + (( (1 + (I / Ki)) ) * B))) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37
uniprot:Q9BKL3 [dTTP]

ATP + Thymidine -> ADP + dTMP
(( (V * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37
uniprot:Q8UVZ9 [dCTP]

2'_Deoxycytidine + ATP -> ADP + dCMP
(( (V * B) ) / (( (Km * (1 + (I / Ki))) ) + B)) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37
uniprot:Q8UVZ9 [dCTP]

ATP + 2'_Deoxycytidine -> dCMP + ADP
(( (V * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37
uniprot:Q8UVZ9 [dTTP]

2'_Deoxycytidine + ATP -> dCMP + ADP
(( (V * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 50 mM Tris/HCl, 5 mM MgCl2, 100 KCl, 0.5 mM phosphoenolpyruvate, 2.2 units/ml pyruvate kinase, 3.1 units/ml lactate dehydrogenase, 0.15 mM NADH
PH: 7.8
Temperature: 37

Pathways

pathway id name
Reactome:R-DME-73614 Pyrimidine salvage
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-15869 Nucleotide metabolism
Reactome:R-DME-8956321 Nucleotide salvage