EC: 2.6.1.13

ornithine aminotransferase (L-ornithine:2-oxo-acid aminotransferase)

enzyme lambda metabolic reaction experiment
uniprot:P04182 [Pyridoxal phosphate]

L_Ornithine + 2_Oxoglutarate -> L_Glutamate + L_Glutamate_5_semialdehyde		 (( (Vmax * C) ) / (Km_C + C)) buffer: 500 mM Phosphate
PH: 8
Temperature: 37
uniprot:P04182 [ornithine aminotransferase(Enzyme) wildtype]

L_Ornithine + 2_Oxoglutarate -> L_Glutamate + L_Glutamate_5_semialdehyde		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Potassium phosphate, 0.11 mM NADH, 0.16 units/ml delta1-Pyrroline 5-carboxylate reductase, 6.66 nM Pyridoxal phosphate
PH: 7.5
Temperature: 36
uniprot:P04182 [ornithine aminotransferase(Enzyme) wildtype]

2_Oxoglutarate + L_Ornithine -> L_Glutamate_5_semialdehyde + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Potassium phosphate, 0.11 mM NADH, 0.16 units/ml delta1-Pyrroline 5-carboxylate reductase, 6.66 nM Pyridoxal phosphate
PH: 7.5
Temperature: 36
uniprot:P04181 [ornithine aminotransferase(Enzyme) wildtype]

2_Oxoglutarate + L_Ornithine -> L_Glutamate_5_semialdehyde + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Potassium phosphate, 0.11 mM NADH, 0.16 units/ml delta1-Pyrroline 5-carboxylate reductase, 6.66 nM Pyridoxal phosphate
PH: 7.5
Temperature: 36
uniprot:P04181 [ornithine aminotransferase(Enzyme) wildtype]

L_Ornithine + 2_Oxoglutarate -> L_Glutamate_5_semialdehyde + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Potassium phosphate, 0.11 mM NADH, 0.16 units/ml delta1-Pyrroline 5-carboxylate reductase, 6.66 nM Pyridoxal phosphate
PH: 7.5
Temperature: 36
uniprot:P04181 [ornithine aminotransferase(Enzyme) wildtype]

L_Ornithine + 2_Oxoglutarate -> L_Glutamate_5_semialdehyde + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Potassium phosphate, 0.11 mM NADH, 0.16 units/ml delta1-Pyrroline 5-carboxylate reductase, 6.66 nM Pyridoxal phosphate
PH: 7.5
Temperature: 36
uniprot:P04181 [ornithine aminotransferase(Enzyme) wildtype]

2_Oxoglutarate + L_Ornithine -> L_Glutamate + L_Glutamate_5_semialdehyde		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Potassium phosphate, 0.11 mM NADH, 0.16 units/ml delta1-Pyrroline 5-carboxylate reductase, 6.66 nM Pyridoxal phosphate
PH: 7.5
Temperature: 36
uniprot:P04181 [ornithine aminotransferase(Enzyme) wildtype]

L_Ornithine + 2_Oxoglutarate -> L_Glutamate_5_semialdehyde + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Potassium phosphate, 0.11 mM NADH, 0.16 units/ml delta1-Pyrroline 5-carboxylate reductase, 6.66 nM Pyridoxal phosphate
PH: 7.5
Temperature: 36
uniprot:P04182 [ornithine aminotransferase(Enzyme) wildtype]

L_Ornithine + 2_Oxoglutarate -> L_Glutamate + L_Glutamate_5_semialdehyde		 (( (Vmax * B) ) / (Km_B + B)) buffer: 500 mM Phosphate, 50 µg/ml Pyridoxal phosphate
PH: 8
Temperature: 37
uniprot:P04182 [ornithine aminotransferase(Enzyme) wildtype]

2_Oxoglutarate + L_Ornithine -> L_Glutamate_5_semialdehyde + L_Glutamate		 (( (Vmax * A) ) / (Km_A + A)) buffer: 500 mM Phosphate, 50 µg/ml Pyridoxal phosphate
PH: 8
Temperature: 37
uniprot:P04182 [ornithine aminotransferase(Enzyme) wildtype]

L_Ornithine + 2_Oxoglutarate -> L_Glutamate + L_Glutamate_5_semialdehyde		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Potassium phosphate, 0.11 mM NADH, 0.16 units/ml delta1-Pyrroline 5-carboxylate reductase, 6.66 nM Pyridoxal phosphate
PH: 7.5
Temperature: 36
uniprot:P04182 [ornithine aminotransferase(Enzyme) wildtype]

L_Ornithine + 2_Oxoglutarate -> L_Glutamate_5_semialdehyde + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Potassium phosphate, 0.11 mM NADH, 0.16 units/ml delta1-Pyrroline 5-carboxylate reductase, 6.66 nM Pyridoxal phosphate
PH: 7.5
Temperature: 36
uniprot:P04181 [ornithine aminotransferase(Enzyme) wildtype]

2_Oxoglutarate + L_Ornithine -> L_Glutamate_5_semialdehyde + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Potassium phosphate, 0.11 mM NADH, 0.16 units/ml delta1-Pyrroline 5-carboxylate reductase, 6.66 nM Pyridoxal phosphate
PH: 7.5
Temperature: 36
uniprot:P04181 [ornithine aminotransferase(Enzyme) wildtype]

L_Ornithine + 2_Oxoglutarate -> L_Glutamate + L_Glutamate_5_semialdehyde		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Potassium phosphate, 0.11 mM NADH, 0.16 units/ml delta1-Pyrroline 5-carboxylate reductase, 6.66 nM Pyridoxal phosphate
PH: 7.5
Temperature: 36
uniprot:P04181 [ornithine aminotransferase(Enzyme) wildtype]

L_Ornithine + 2_Oxoglutarate -> L_Glutamate_5_semialdehyde + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Potassium phosphate, 0.11 mM NADH, 0.16 units/ml delta1-Pyrroline 5-carboxylate reductase, 6.66 nM Pyridoxal phosphate
PH: 7.5
Temperature: 36
uniprot:P04182 [ornithine aminotransferase(Enzyme) wildtype]

L_Ornithine + 2_Oxoglutarate -> L_Glutamate + L_Glutamate_5_semialdehyde		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Potassium phosphate, 0.11 mM NADH, 0.16 units/ml delta1-Pyrroline 5-carboxylate reductase, 6.66 nM Pyridoxal phosphate
PH: 7.5
Temperature: 36
uniprot:P04182 [ornithine aminotransferase(Enzyme) wildtype]

2_Oxoglutarate + L_Ornithine -> L_Glutamate_5_semialdehyde + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Potassium phosphate, 0.11 mM NADH, 0.16 units/ml delta1-Pyrroline 5-carboxylate reductase, 6.66 nM Pyridoxal phosphate
PH: 7.5
Temperature: 36
uniprot:P04182 [ornithine aminotransferase(Enzyme) wildtype]

2_Oxoglutarate + L_Ornithine -> L_Glutamate + L_Glutamate_5_semialdehyde		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Potassium phosphate, 0.11 mM NADH, 0.16 units/ml delta1-Pyrroline 5-carboxylate reductase, 6.66 nM Pyridoxal phosphate
PH: 7.5
Temperature: 36
uniprot:P04182 [ornithine aminotransferase(Enzyme) wildtype]

L_Ornithine + 2_Oxoglutarate -> L_Glutamate_5_semialdehyde + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 33.3 mM Potassium phosphate, 0.11 mM NADH, 0.16 units/ml delta1-Pyrroline 5-carboxylate reductase, 6.66 nM Pyridoxal phosphate
PH: 7.5
Temperature: 36
uniprot:P04182 [Pyridoxal phosphate]

2_Oxoglutarate + L_Ornithine -> 3,4_Dihydro_2H_Pyrrole_2_carboxylate + L_Glutamate		 (( (Vmax * B) ) / (Km + B)) buffer: 500 mM Phosphate
PH: 8
Temperature: 37
uniprot:P04182 [Pyridoxal phosphate]

2_Oxoglutarate + L_Ornithine -> 3,4_Dihydro_2H_Pyrrole_2_carboxylate + L_Glutamate		 (( (Vmax * B) ) / (Km + B)) buffer: 500 mM Phosphate
PH: 8
Temperature: 37
uniprot:P04182 [Pyridoxal phosphate]

L_Ornithine + 2_Oxoglutarate -> 3,4_Dihydro_2H_Pyrrole_2_carboxylate + L_Glutamate		 (( (Vmax * B) ) / (Km + B)) buffer: 500 mM Phosphate
PH: 8
Temperature: 37
uniprot:P04182 [Pyridoxal phosphate]

L_Ornithine + 2_Oxoglutarate -> L_Glutamate + 3,4_Dihydro_2H_Pyrrole_2_carboxylate		 (( (Vmax * A) ) / (Km + A)) buffer: 500 mM Phosphate
PH: 8
Temperature: 37
uniprot:P04182 [Pyridoxal phosphate]

L_Ornithine + 2_Oxoglutarate -> L_Glutamate + 3,4_Dihydro_2H_Pyrrole_2_carboxylate		 (( (Vmax * A) ) / (Km + A)) buffer: 500 mM Phosphate
PH: 8
Temperature: 37
uniprot:P04182 [Pyridoxal phosphate]

2_Oxoglutarate + L_Ornithine -> 3,4_Dihydro_2H_Pyrrole_2_carboxylate + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 500 mM Phosphate
PH: 8
Temperature: 37
uniprot:P04182 [ornithine aminotransferase(Enzyme) wildtype]

2_Oxoglutarate + L_Ornithine -> L_Glutamate + 3,4_Dihydro_2H_Pyrrole_2_carboxylate		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Potassium phosphate
PH: 7.1
Temperature: 37
uniprot:P04182 [ornithine aminotransferase(Enzyme) wildtype]

L_Ornithine + 2_Oxoglutarate -> 3,4_Dihydro_2H_Pyrrole_2_carboxylate + L_Glutamate		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Potassium phosphate
PH: 7.1
Temperature: 37
uniprot:P04182 [ornithine aminotransferase(Enzyme) wildtype]

Pyruvate + L_Ornithine -> 3,4_Dihydro_2H_Pyrrole_2_carboxylate + L_Alanine		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Potassium phosphate
PH: 7.1
Temperature: 37
uniprot:P04182 [ornithine aminotransferase(Enzyme) wildtype]

L_Ornithine + Pyruvate -> 3,4_Dihydro_2H_Pyrrole_2_carboxylate + L_Alanine		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Potassium phosphate
PH: 7.1
Temperature: 37
uniprot:P04182 [ornithine aminotransferase(Enzyme) wildtype]

L_Ornithine + Glyoxylate -> Glycine + 3,4_Dihydro_2H_Pyrrole_2_carboxylate		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Potassium phosphate, 2 mg/ml EDTA
PH: 7.1
Temperature: 37
uniprot:P04182 [ornithine aminotransferase(Enzyme) wildtype]

Glyoxylate + L_Ornithine -> 3,4_Dihydro_2H_Pyrrole_2_carboxylate + Glycine		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Potassium phosphate, 2 mg/ml EDTA
PH: 7.1
Temperature: 37
uniprot:P04182 [Pyridoxal phosphate]

2_Oxoglutarate + L_Ornithine -> L_Glutamate_5_semialdehyde + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 8
Temperature: 37
uniprot:P04182 [Pyridoxal phosphate]

2_Oxoglutarate + L_Ornithine -> L_Glutamate_5_semialdehyde + L_Glutamate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Potassium phosphate
PH: 8
Temperature: 37
uniprot:P04182 [Pyridoxal phosphate]

2_Oxoglutarate + L_Ornithine -> L_Glutamate_5_semialdehyde + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 8
Temperature: 37
uniprot:P04182 [Pyridoxal phosphate]

L_Ornithine + 2_Oxoglutarate -> L_Glutamate + L_Glutamate_5_semialdehyde		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Potassium phosphate
PH: 8
Temperature: 37
uniprot:P04182 [Pyridoxal phosphate]

L_Ornithine + 2_Oxoglutarate -> L_Glutamate_5_semialdehyde + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate
PH: 8
Temperature: 37
uniprot:P04182 [Pyridoxal phosphate]

2_Oxoglutarate + L_Ornithine -> L_Glutamate + L_Glutamate_5_semialdehyde		 (( (Vmax * M) ) / (Km + M)) buffer: 50 mM Potassium phosphate
PH: 8
Temperature: 37
uniprot:P04182 [Pyridoxal phosphate]

2_Oxoglutarate + L_Ornithine -> L_Glutamate_5_semialdehyde + L_Glutamate		 (( (Vmax * M) ) / (Km + M)) buffer: 50 mM Potassium phosphate
PH: 8
Temperature: 37
uniprot:P04182 [Pyridoxal phosphate]

2_Oxoglutarate + L_Ornithine -> L_Glutamate + L_Glutamate_5_semialdehyde		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Potassium phosphate
PH: 8
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-5004 superpathway of L-citrulline metabolism
BioCyc:META_ARG-PRO-PWY L-arginine degradation VI (arginase 2 pathway)
BioCyc:YEAST_CITRULBIO-PWY citrulline biosynthesis
BioCyc:ANTHRA_ARG-PRO-PWY L-arginine degradation VI (arginase 2 pathway)
BioCyc:ANTHRA_PWY-3341 L-proline biosynthesis III
BioCyc:BSUB_ARGASEDEG-PWY L-arginine degradation I (arginase pathway)
BioCyc:BSUB_PWY-6344 L-ornithine degradation II (Stickland reaction)
BioCyc:META_PWY-6922 L-Nδ-acetylornithine biosynthesis
BioCyc:META_ARGASEDEG-PWY L-arginine degradation I (arginase pathway)
BioCyc:YEAST_ARG-PRO-PWY arginine degradation VI (arginase 2 pathway)
BioCyc:PLASMO_ARG+POLYAMINE-SYN superpathway of arginine and polyamine biosynthesis
BioCyc:ANTHRA_ARGASEDEG-PWY L-arginine degradation I (arginase pathway)
BioCyc:MOUSE_PWY-4981 proline biosynthesis II (from arginine)
BioCyc:MOUSE_PWY-5004 superpathway of citrulline metabolism
BioCyc:MOUSE_ARG-PRO-PWY arginine degradation VI (arginase 2 pathway)
BioCyc:META_CITRULBIO-PWY L-citrulline biosynthesis
BioCyc:META_PWY-3341 L-proline biosynthesis III
BioCyc:PLASMO_PWY-5004 superpathway of citrulline metabolism
BioCyc:PLASMO_CITRULBIO-PWY citrulline biosynthesis
BioCyc:PLASMO_PWY-5022 4-aminobutyrate degradation V
BioCyc:PLASMO_PWY-5188 tetrapyrrole biosynthesis I
BioCyc:ANTHRA_PWY-6344 L-ornithine degradation II (Stickland reaction)
BioCyc:ANTHRA_ARGORNPROST-PWY arginine, ornithine and proline interconversion
BioCyc:MOUSE_ARGASEDEG-PWY arginine degradation I (arginase pathway)
BioCyc:MOUSE_ARGININE-SYN4-PWY arginine biosynthesis IV
BioCyc:BSUB_ARG-PRO-PWY L-arginine degradation VI (arginase 2 pathway)
BioCyc:META_ARGININE-SYN4-PWY L-ornithine biosynthesis II
BioCyc:PLASMO_GLUTORN-PWY ornithine biosynthesis
BioCyc:PLASMO_PWY-5154 arginine biosynthesis III
BioCyc:PLASMO_ARGSYN-PWY arginine biosynthesis I
BioCyc:PLASMO_ARGSYNBSUB-PWY arginine biosynthesis II (acetyl cycle)
BioCyc:PLASMO_PWY-3941 β-alanine biosynthesis II
BioCyc:PLASMO_ARG-PRO-PWY arginine degradation VI (arginase 2 pathway)
BioCyc:MOUSE_CITRULBIO-PWY citrulline biosynthesis
Plant Reactome:R-OSA-1119495 Citrulline biosynthesis
Plant Reactome:R-OSA-1119289 Arginine degradation
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Plant Reactome:R-OSA-2744343 Amino acid metabolism
Plant Reactome:R-OSA-1119318 Proline biosynthesis V (from arginine)
Plant Reactome:R-OSA-5655122 Amino acid biosynthesis
Reactome:R-RNO-8964539 Glutamate and glutamine metabolism
Reactome:R-SCE-8964539 Glutamate and glutamine metabolism
Reactome:R-CEL-8964539 Glutamate and glutamine metabolism
Reactome:R-SPO-8964539 Glutamate and glutamine metabolism
Reactome:R-DDI-8964539 Glutamate and glutamine metabolism
Reactome:R-MMU-8964539 Glutamate and glutamine metabolism
Reactome:R-PFA-8964539 Glutamate and glutamine metabolism
Reactome:R-DME-8964539 Glutamate and glutamine metabolism
Reactome:R-HSA-8964539 Glutamate and glutamine metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SCE-71291 Amino acid and derivative metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-71291 Amino acid and derivative metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SPO-71291 Amino acid and derivative metabolism
Reactome:R-CEL-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-PFA-1430728 Metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-PFA-71291 Amino acid and derivative metabolism
WikiPathways:WP4595 Urea cycle and associated pathways
WikiPathways:WP497 Urea cycle and metabolism of amino groups
PathBank:SMP0120569 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0000020 Arginine and Proline Metabolism
PathBank:SMP0000362 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0000188 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0000504 Creatine Deficiency, Guanidinoacetate Methyltransferase Deficiency
PathBank:SMP0063596 Arginine and Proline Metabolism
PathBank:SMP0120499 Hyperprolinemia Type I
PathBank:SMP0120539 Prolinemia Type II
PathBank:SMP0120572 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0000207 Prolidase Deficiency (PD)
PathBank:SMP0000208 Prolinemia Type II
PathBank:SMP0000507 L-Arginine:Glycine Amidinotransferase Deficiency
PathBank:SMP0002416 Arginine Metabolism
PathBank:SMP0120501 Hyperprolinemia Type II
PathBank:SMP0120541 Prolidase Deficiency (PD)
PathBank:SMP0120570 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0000360 Hyperprolinemia Type II
PathBank:SMP0000363 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0000505 Hyperornithinemia with Gyrate Atrophy (HOGA)
PathBank:SMP0002303 Glutamate Metabolism
PathBank:SMP0012004 Arginine and Proline Metabolism
PathBank:SMP0120454 Arginine: Glycine Amidinotransferase Deficiency (AGAT Deficiency)
PathBank:SMP0120490 Guanidinoacetate Methyltransferase Deficiency (GAMT Deficiency)
PathBank:SMP0120538 Ornithine Aminotransferase Deficiency (OAT Deficiency)
PathBank:SMP0120571 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0000361 Hyperprolinemia Type I
PathBank:SMP0000506 Hyperornithinemia-Hyperammonemia-Homocitrullinuria [HHH-syndrome]
PathBank:SMP0087178 Arginine and Proline Metabolism