EC: 2.4.1.4

amylosucrase (sucrose:(1->4)-alpha-D-glucan 4-alpha-D-glucosyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q9ZEU2 [amylosucrase(Enzyme) wildtype]

Sucrose + H2O -> D_Fructose + D_Glucose
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 7
Temperature: 30
uniprot:Q9ZEU2 [amylosucrase(Enzyme) mutant 002-G1]

(1,4_alpha_D_Glucosyl)n + Sucrose -> D_Fructose + (1,4_alpha_D_Glucosyl)n
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 7
Temperature: 30
uniprot:Q9ZEU2 [amylosucrase(Enzyme) mutant 002-G1]

Sucrose + (1,4_alpha_D_Glucosyl)n -> (1,4_alpha_D_Glucosyl)n + D_Fructose
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 7
Temperature: 30
uniprot:Q9ZEU2 [amylosucrase(Enzyme) mutant 001-D2]

Sucrose + H2O -> D_Fructose + D_Glucose
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 7
Temperature: 30
uniprot:Q9ZEU2 [amylosucrase(Enzyme) mutant 002-G1]

H2O + Sucrose -> D_Glucose + D_Fructose
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 7
Temperature: 30
uniprot:Q9ZEU2 [amylosucrase(Enzyme) wildtype]

H2O + Sucrose -> D_Glucose + D_Fructose
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 7
Temperature: 30
uniprot:Q9ZEU2 [amylosucrase(Enzyme) mutant 002-G1]

Sucrose + H2O -> D_Fructose + D_Glucose
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 7
Temperature: 30
uniprot:Q9ZEU2 [amylosucrase(Enzyme) mutant 001-D2]

H2O + Sucrose -> D_Glucose + D_Fructose
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 7
Temperature: 30
uniprot:Q9ZEU2 [amylosucrase(Enzyme) mutant 001-D2]

(1,4_alpha_D_Glucosyl)n + Sucrose -> (1,4_alpha_D_Glucosyl)n + D_Fructose
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 7
Temperature: 30
uniprot:Q9ZEU2 [amylosucrase(Enzyme) mutant 001-D2]

(1,4_alpha_D_Glucosyl)n + Sucrose -> D_Fructose + (1,4_alpha_D_Glucosyl)n
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 7
Temperature: 30
uniprot:Q9ZEU2 [amylosucrase(Enzyme) wildtype]

(1,4_alpha_D_Glucosyl)n + Sucrose -> (1,4_alpha_D_Glucosyl)n + D_Fructose
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 7
Temperature: 30
uniprot:Q9ZEU2 [amylosucrase(Enzyme) wildtype]

(1,4_alpha_D_Glucosyl)n + Sucrose -> (1,4_alpha_D_Glucosyl)n + D_Fructose
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 7
Temperature: 30

Pathways

pathway id name