EC: 2.3.1.35

glutamate N-acetyltransferase (N2-acetyl-L-ornithine:L-glutamate N-acetyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q3C251 [glutamate N-acetyltransferase(Enzyme) wildtype 2 alpha and 2 beta chains]

L_Glutamate + N2_Acetyl_L_ornithine -> N_Acetyl_L_glutamate + L_Ornithine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mm potassium phosphate
PH: 7
Temperature: 36
uniprot:Q3C251 [glutamate N-acetyltransferase(Enzyme) wildtype 2 alpha and 2 beta chains]

N2_Acetyl_L_ornithine + L_Glutamate -> L_Ornithine + N_Acetyl_L_glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mm potassium phosphate
PH: 7
Temperature: 36
uniprot:Q57645 [glutamate N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

N2_Acetyl_L_ornithine + L_Glutamate -> N_Acetyl_L_glutamate + L_Ornithine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M MES, 0.1 M Pipes, 0.1 M Tris, 0.1 M glycine, 0.1 M K2HPO4
PH: 8
Temperature: 95
uniprot:Q57645 [glutamate N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

L_Glutamate + N2_Acetyl_L_ornithine -> L_Ornithine + N_Acetyl_L_glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M MES, 0.1 M Pipes, 0.1 M Tris, 0.1 M glycine, 0.1 M K2HPO4
PH: 8
Temperature: 95
uniprot:Q9Z4S1 [glutamate N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

N2_Acetyl_L_ornithine + L_Glutamate -> N_Acetyl_L_glutamate + L_Ornithine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M MES, 0.1 M Pipes, 0.1 M Tris, 0.1 M glycine, 0.1 M K2HPO4
PH: 8
Temperature: 90
uniprot:Q9Z4S1 [glutamate N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

L_Glutamate + N2_Acetyl_L_ornithine -> N_Acetyl_L_glutamate + L_Ornithine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M MES, 0.1 M Pipes, 0.1 M Tris, 0.1 M glycine, 0.1 M K2HPO4
PH: 8
Temperature: 90
- [glutamate N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

L_Glutamate + N2_Acetyl_L_ornithine -> L_Ornithine + N_Acetyl_L_glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M MES, 0.1 M Pipes, 0.1 M Tris, 0.1 M glycine, 0.1 M K2HPO4
PH: 8
Temperature: 75
- [glutamate N-acetyltransferase(Enzyme) wildtype]

N2_Acetyl_L_ornithine + L_Glutamate -> L_Ornithine + N_Acetyl_L_glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M MES, 0.1 M Pipes, 0.1 M Tris, 0.1 M glycine, 0.1 M K2HPO4
PH: 8
Temperature: 75
- [glutamate N-acetyltransferase(Enzyme) wildtype C-terminal His-tag]

L_Glutamate + N2_Acetyl_L_ornithine -> L_Ornithine + N_Acetyl_L_glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M MES, 0.1 M Pipes, 0.1 M Tris, 0.1 M glycine, 0.1 M K2HPO4
PH: 8
Temperature: 75
uniprot:Q07908 [glutamate N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

L_Glutamate + N2_Acetyl_L_ornithine -> L_Ornithine + N_Acetyl_L_glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M MES, 0.1 M Pipes, 0.1 M Tris, 0.1 M glycine, 0.1 M K2HPO4
PH: 8
Temperature: 75
uniprot:Q07908 [glutamate N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

N2_Acetyl_L_ornithine + L_Glutamate -> N_Acetyl_L_glutamate + L_Ornithine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M MES, 0.1 M Pipes, 0.1 M Tris, 0.1 M glycine, 0.1 M K2HPO4
PH: 8
Temperature: 75
uniprot:Q9Z4S1 [glutamate N-acetyltransferase(Enzyme) wildtype N-terminal His-tag]

L_Glutamate + N2_Acetyl_L_ornithine -> N_Acetyl_L_glutamate + L_Ornithine		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M MES, 0.1 M Pipes, 0.1 M Tris, 0.1 M glycine, 0.1 M K2HPO4
PH: 8
Temperature: 90
uniprot:Q04728 [glutamate N-acetyltransferase(Enzyme) wildtype]

N2_Acetyl_L_ornithine + L_Glutamate -> N_Acetyl_L_glutamate + L_Ornithine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate, 10 mM ATP, 20 mM MgCl2, 0.2 mM NADPH, 10 µg/ml N-Acetyl-glutamate kinase, 10 µg/ml N-Acetyl-gamma-glutamyl-phosphate reductase
PH: 7
Temperature: 30
uniprot:Q04728 [glutamate N-acetyltransferase(Enzyme) wildtype]

N2_Acetyl_L_ornithine + L_Glutamate -> L_Ornithine + N_Acetyl_L_glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM potassium phosphate, 10 mM ATP, 20 mM MgCl2, 0.2 mM NADPH, 10 µg/ml N-Acetyl-glutamate kinase, 10 µg/ml N-Acetyl-gamma-glutamyl-phosphate reductase
PH: 7
Temperature: 30
uniprot:Q9LCS7 [glutamate N-acetyltransferase(Enzyme) wildtype isozyme ArgJ]

L_Glutamate + N2_Acetyl_L_ornithine -> N_Acetyl_L_glutamate + L_Ornithine		 (( (( (Vmax * A) ) * B) ) / (( (( (Km_A * B) ) + (Km_B * A)) ) + (A * B))) buffer: 100 mM Tris-HCl
PH: 8.5
Temperature: 37
uniprot:Q9LCS7 [glutamate N-acetyltransferase(Enzyme) wildtype isozyme Oat2]

N2_Acetyl_L_ornithine + L_Glutamate -> L_Ornithine + N_Acetyl_L_glutamate		 (( (( (Vmax * A) ) * B) ) / (( (( (Km_A * B) ) + (Km_B * A)) ) + (A * B))) buffer: 100 mM Tris-HCl
PH: 8.5
Temperature: 37

Pathways

pathway id name
BioCyc:ARA_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:MTBCDC1551_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:ANTHRA_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:AGRO_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:MTBH37RV_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:MTBCDC1551_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:CAULO_GLUTORN-PWY ornithine biosynthesis
BioCyc:ANTHRA_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:ANTHRA_GLUTORN-PWY L-ornithine biosynthesis I
BioCyc:AGRO_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:CORYNE_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:ARA_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
BioCyc:CAULO_ARGSYNBSUB-PWY arginine biosynthesis II (acetyl cycle)
BioCyc:YEAST_ARGSYNBSUB-PWY arginine biosynthesis
BioCyc:ARA_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:MTBCDC1551_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:CAULO_ARGSYN-PWY arginine biosynthesis I
BioCyc:BSUB_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
BioCyc:PCHR_ARGSYNBSUB-PWY arginine biosynthesis II (acetyl cycle)
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Plant Reactome:R-OSA-2744343 Amino acid metabolism
Plant Reactome:R-OSA-1119263 Arginine biosynthesis
Plant Reactome:R-OSA-5655122 Amino acid biosynthesis
Plant Reactome:R-OSA-1119622 Arginine biosynthesis II (acetyl cycle)
PlantCyc:ARA_ARGSYN-PWY L-arginine biosynthesis I (via L-ornithine)
PlantCyc:ARA_GLUTORN-PWY L-ornithine biosynthesis I
PlantCyc:ARA_ARGSYNBSUB-PWY L-arginine biosynthesis II (acetyl cycle)
PathBank:SMP0002420 Proline Metabolism
PathBank:SMP0002416 Arginine Metabolism