EC: 1.3.8.6

glutaryl-CoA dehydrogenase (ETF) (glutaryl-CoA:electron-transfer flavoprotein 2,3-oxidoreductase (decarboxylating))

enzyme lambda metabolic reaction experiment
uniprot:P81140 [glutaryl-CoA dehydrogenase(Enzyme) wildtype]

Glutaryl_CoA + FAD -> FADH2 + CO2 + Crotonoyl_CoA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Potassium phosphate, 1 mM Methylene blue
PH: 7.5
Temperature: 37
uniprot:P81140 [glutaryl-CoA dehydrogenase(Enzyme) wildtype]

Electron_transferring_flavoprotein + Glutaryl_CoA -> Reduced_electron_transferring_flavoprotein + Crotonoyl_CoA + CO2		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 8 mM Tris-HCl, 1 mg/ml BSA
PH: 7
Temperature: 25
uniprot:P81140 [glutaryl-CoA dehydrogenase(Enzyme) wildtype]

Electron_transferring_flavoprotein + Glutaryl_CoA -> Crotonoyl_CoA + CO2 + Reduced_electron_transferring_flavoprotein		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 8 mM Tris-HCl, 1 mg/ml BSA
PH: 7
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) mutant R94G]

Glutaryl_CoA + Acceptor -> Crotonoyl_CoA + CO2 + Reduced_acceptor		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM potassium phosphate
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) wildtype]

Glutaryl_CoA + Acceptor -> CO2 + Crotonoyl_CoA + Reduced_acceptor		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM potassium phosphate
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) mutant S98A]

Glutaryl_CoA + Acceptor -> Crotonoyl_CoA + CO2 + Reduced_acceptor		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM potassium phosphate
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) mutant R94Q]

Acceptor + Glutaryl_CoA -> Crotonoyl_CoA + Reduced_acceptor + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM potassium phosphate
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) wildtype]

Hexanoyl_CoA + Acceptor -> Reduced_acceptor + trans_Hex_2_enoyl_CoA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM potassium phosphate
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) mutant S98A]

Hexanoyl_CoA + Acceptor -> trans_Hex_2_enoyl_CoA + Reduced_acceptor		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM potassium phosphate
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) mutant R94Q]

Acceptor + Hexanoyl_CoA -> trans_Hex_2_enoyl_CoA + Reduced_acceptor		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM potassium phosphate
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) mutant R94G]

Acceptor + Hexanoyl_CoA -> trans_Hex_2_enoyl_CoA + Reduced_acceptor		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM potassium phosphate
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) wildtype]

Glutaramyl_CoA + Acceptor -> Reduced_acceptor + 5_Amino_5_oxopent_2_enoate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM potassium phosphate
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) mutant S98A]

Glutaramyl_CoA + Acceptor -> Reduced_acceptor + 5_Amino_5_oxopent_2_enoate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM potassium phosphate
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) mutant R94G]

Acceptor + Glutaramyl_CoA -> Reduced_acceptor + 5_Amino_5_oxopent_2_enoate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM potassium phosphate
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) mutant R94Q]

Glutaramyl_CoA + Acceptor -> 5_Amino_5_oxopent_2_enoate + Reduced_acceptor		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM potassium phosphate
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) wildtype]

Acceptor + Glutaryl_CoA -> Crotonoyl_CoA + CO2 + Reduced_acceptor		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM Tris-HCl
PH: 8
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) wildtype]

Electron_transferring_flavoprotein + Glutaryl_CoA -> Reduced_electron_transferring_flavoprotein + CO2 + Crotonoyl_CoA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate, 50 µM DCPIP
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) wildtype]

Electron_transferring_flavoprotein + Glutaryl_CoA -> Reduced_electron_transferring_flavoprotein + Crotonoyl_CoA + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate, 50 µM DCPIP
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) mutant Ala421Val]

Electron_transferring_flavoprotein + Glutaryl_CoA -> Reduced_electron_transferring_flavoprotein + Crotonoyl_CoA + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate, 50 µM DCPIP
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) mutant Thr429Met]

Electron_transferring_flavoprotein + Glutaryl_CoA -> CO2 + Crotonoyl_CoA + Reduced_electron_transferring_flavoprotein		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate, 50 µM DCPIP
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) mutant Ala421Val]

Electron_transferring_flavoprotein + Glutaryl_CoA -> Reduced_electron_transferring_flavoprotein + Crotonoyl_CoA + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate, 50 µM DCPIP
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) mutant Thr429Met]

Glutaryl_CoA + Electron_transferring_flavoprotein -> Reduced_electron_transferring_flavoprotein + Crotonoyl_CoA + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate, 50 µM DCPIP
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) mutant Ala421Thr]

Electron_transferring_flavoprotein + Glutaryl_CoA -> Crotonoyl_CoA + Reduced_electron_transferring_flavoprotein + CO2		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate, 50 µM DCPIP
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) mutant Ala433Val]

Electron_transferring_flavoprotein + Glutaryl_CoA -> CO2 + Reduced_electron_transferring_flavoprotein + Crotonoyl_CoA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate, 50 µM DCPIP
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) mutant Ala433Val]

Electron_transferring_flavoprotein + Glutaryl_CoA -> CO2 + Crotonoyl_CoA + Reduced_electron_transferring_flavoprotein		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate, 50 µM DCPIP
PH: 7.6
Temperature: 25
uniprot:Q92947 [glutaryl-CoA dehydrogenase(Enzyme) mutant Ala421Thr]

Electron_transferring_flavoprotein + Glutaryl_CoA -> Reduced_electron_transferring_flavoprotein + CO2 + Crotonoyl_CoA		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 10 mM Potassium phosphate, 50 µM DCPIP
PH: 7.6
Temperature: 25

Pathways

pathway id name
BioCyc:HUMAN_PWY-5177 glutaryl-CoA degradation
BioCyc:HUMAN_TRYPTOPHAN-DEGRADATION-1 tryptophan degradation
BioCyc:HUMAN_PWY66-401 superpathway of tryptophan utilization
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-CEL-71064 Lysine catabolism
Reactome:R-HSA-71064 Lysine catabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-71064 Lysine catabolism
Reactome:R-BTA-71064 Lysine catabolism
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-CEL-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-71064 Lysine catabolism
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP5355 Metabolic Epileptic Disorders
WikiPathways:WP4519 Cerebral organic acidurias, including diseases
PathBank:SMP0120476 Ethylmalonic Encephalopathy
PathBank:SMP0120484 Glutaric Aciduria Type I
PathBank:SMP0120520 Short-Chain Acyl-CoA Dehydrogenase Deficiency (SCAD Deficiency)
PathBank:SMP0120593 Hyperlysinemia II or Saccharopinuria
PathBank:SMP0120605 Very-Long-Chain Acyl-CoA Dehydrogenase Deficiency (VLCAD)
PathBank:SMP0120609 Trifunctional Protein Deficiency
PathBank:SMP0000051 Fatty Acid Metabolism
PathBank:SMP0000235 Short-Chain Acyl-CoA Dehydrogenase Deficiency (SCAD Deficiency)
PathBank:SMP0000239 Saccharopinuria/Hyperlysinemia II
PathBank:SMP0000528 Hyperlysinemia II or Saccharopinuria
PathBank:SMP0000540 Very-Long-Chain Acyl-CoA Dehydrogenase Deficiency (VLCAD)
PathBank:SMP0087430 Fatty Acid Metabolism
PathBank:SMP0120592 Hyperlysinemia I, Familial
PathBank:SMP0120604 Long Chain Acyl-CoA Dehydrogenase Deficiency (LCAD)
PathBank:SMP0000185 Glutaric Aciduria Type I
PathBank:SMP0000186 Glutaric Aciduria Type I
PathBank:SMP0000527 Hyperlysinemia I, Familial
PathBank:SMP0000539 Long Chain Acyl-CoA Dehydrogenase Deficiency (LCAD)
PathBank:SMP0000571 Pyridoxine Dependency with Seizures
PathBank:SMP0087172 Fatty Acid Metabolism
PathBank:SMP0120606 Carnitine Palmitoyl Transferase Deficiency II
PathBank:SMP0000541 Carnitine Palmitoyl Transferase Deficiency II
PathBank:SMP0000545 Trifunctional Protein Deficiency
PathBank:SMP0000719 2-Aminoadipic 2-Oxoadipic Aciduria
PathBank:SMP0087203 Lysine Degradation
PathBank:SMP0120651 2-Aminoadipic 2-Oxoadipic Aciduria
PathBank:SMP0120526 Saccharopinuria/Hyperlysinemia II
PathBank:SMP0120603 Carnitine Palmitoyl Transferase Deficiency I
PathBank:SMP0120607 Medium Chain Acyl-CoA Dehydrogenase Deficiency (MCAD)
PathBank:SMP0120635 Pyridoxine Dependency with Seizures
PathBank:SMP0000037 Lysine Degradation
PathBank:SMP0000181 Ethylmalonic Encephalopathy
PathBank:SMP0000538 Carnitine Palmitoyl Transferase Deficiency I
PathBank:SMP0000542 Medium Chain Acyl-CoA Dehydrogenase Deficiency (MCAD)
PathBank:SMP0063589 Fatty Acid Metabolism
PathBank:SMP0063635 Lysine Degradation